ClpB is the Escherichia coli heat shock protein F84.1
10.1128/jb.173.14.4254-4262.1991
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

ClpB is thought to be involved in proteolysis because of its sequence similarity to the ClpA subunit of the ClpA-ClpP protease. It has recently been shown that ClpP is a heat shock protein. Here we show that ClpB is the Escherichia coli heat shock protein F84.1. The F84.1 protein was overproduced in strains containing the clpB gene on a plasmid and was absent from two-dimensional gels from a clpB null mutation. Besides possessing a slower growth rate at 44 degrees C, the null mutant strain had a higher rate of death at 50 degrees C. We used reverse transcription of in vivo mRNA to show that the clpB gene was expressed from a sigma 32-specific promoter consensus sequence at both 37 and 42 degrees C. We noted that the clpB+ gene also caused the appearance of a second protein spot, F68.5, on two-dimensional gels. This spot was approximately 147 amino acids smaller than F84.1 and most probably is the result of a second translational start on the clpB mRNA. F68.5 can be observed on many published two-dimensional gels of heat-induced E. coli proteins, but the original catalog of 17 heat shock proteins did not include this spot.

Bibliography

Squires, C. L., Pedersen, S., Ross, B. M., & Squires, C. (1991). ClpB is the Escherichia coli heat shock protein F84.1. Journal of Bacteriology, 173(14), 4254–4262.

Authors 4
  1. C L Squires (first)
  2. S Pedersen (additional)
  3. B M Ross (additional)
  4. C Squires (additional)
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Dates
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Created 8 years, 9 months ago (Nov. 7, 2016, 11:33 a.m.)
Deposited 4 years ago (July 29, 2021, 1:52 p.m.)
Indexed 3 weeks, 2 days ago (July 30, 2025, 8:34 p.m.)
Issued 34 years, 1 month ago (July 1, 1991)
Published 34 years, 1 month ago (July 1, 1991)
Published Print 34 years, 1 month ago (July 1, 1991)
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@article{Squires_1991, title={ClpB is the Escherichia coli heat shock protein F84.1}, volume={173}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.173.14.4254-4262.1991}, DOI={10.1128/jb.173.14.4254-4262.1991}, number={14}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Squires, C L and Pedersen, S and Ross, B M and Squires, C}, year={1991}, month=jul, pages={4254–4262} }