Coralline shape of the bacterial nucleoid after cryofixation
10.1128/jb.173.10.3149-3158.1991
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

A new procedure of immunostaining sections of cryofixed and freeze-substituted Escherichia coli shows that DNA extends from its bulk into small ribosome-free spaces throughout the cytoplasm, resulting in a coralline-shaped nucleoid. Low-resolution imaging of a bacterium reconstructed from serial sections demonstrated that the small excrescencies are not resolved. The resulting photograph shows the same features as phase-contrast light micrographs.

Bibliography

Bohrmann, B., Villiger, W., Johansen, R., & Kellenberger, E. (1991). Coralline shape of the bacterial nucleoid after cryofixation. Journal of Bacteriology, 173(10), 3149–3158.

Authors 4
  1. B Bohrmann (first)
  2. W Villiger (additional)
  3. R Johansen (additional)
  4. E Kellenberger (additional)
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Dates
Type When
Created 8 years, 9 months ago (Nov. 7, 2016, 11:17 a.m.)
Deposited 4 years ago (July 29, 2021, 1:51 p.m.)
Indexed 1 year, 3 months ago (May 9, 2024, 12:37 a.m.)
Issued 34 years, 3 months ago (May 1, 1991)
Published 34 years, 3 months ago (May 1, 1991)
Published Print 34 years, 3 months ago (May 1, 1991)
Funders 0

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@article{Bohrmann_1991, title={Coralline shape of the bacterial nucleoid after cryofixation}, volume={173}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.173.10.3149-3158.1991}, DOI={10.1128/jb.173.10.3149-3158.1991}, number={10}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Bohrmann, B and Villiger, W and Johansen, R and Kellenberger, E}, year={1991}, month=may, pages={3149–3158} }