Organization and nucleotide sequences of the Spiroplasma citri genes for ribosomal protein S2, elongation factor Ts, spiralin, phosphofructokinase, pyruvate kinase, and an unidentified protein
10.1128/jb.172.5.2693-2703.1990
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The gene for spiralin, the major membrane protein of the helical mollicute Spiroplasma citri, was cloned in Escherichia coli as a 5-kilobase-pair (kbp) DNA fragment. The complete nucleotide sequence of the 5.0-kbp spiroplasmal DNA fragment was determined (GenBank accession no. M31161). The spiralin gene was identified by the size and amino acid composition of its translational product. Besides the spiralin gene, the spiroplasmal DNA fragment was found to contain five additional open reading frames (ORFs). The translational products of four of these ORFs were identified by their amino acid sequence homologies with known proteins: ribosomal protein S2, elongation factor Ts, phosphofructokinase, and pyruvate kinase, respectively encoded by the genes rpsB, tsf, pfk, and pyk. The product of the fifth ORF remains to be identified and was named protein X (X gene). The order of the above genes was tsf--X--spiralin gene--pfk--pyk. These genes were transcribed in one direction, while the gene for ribosomal protein S2 (rpsB) was transcribed in the opposite direction.

Bibliography

Chevalier, C., Saillard, C., & Bové, J. M. (1990). Organization and nucleotide sequences of the Spiroplasma citri genes for ribosomal protein S2, elongation factor Ts, spiralin, phosphofructokinase, pyruvate kinase, and an unidentified protein. Journal of Bacteriology, 172(5), 2693–2703.

Authors 3
  1. C Chevalier (first)
  2. C Saillard (additional)
  3. J M Bové (additional)
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Dates
Type When
Created 8 years, 10 months ago (Nov. 4, 2016, 12:33 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:48 p.m.)
Indexed 1 month, 1 week ago (July 30, 2025, 8:34 p.m.)
Issued 35 years, 4 months ago (May 1, 1990)
Published 35 years, 4 months ago (May 1, 1990)
Published Print 35 years, 4 months ago (May 1, 1990)
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@article{Chevalier_1990, title={Organization and nucleotide sequences of the Spiroplasma citri genes for ribosomal protein S2, elongation factor Ts, spiralin, phosphofructokinase, pyruvate kinase, and an unidentified protein}, volume={172}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.172.5.2693-2703.1990}, DOI={10.1128/jb.172.5.2693-2703.1990}, number={5}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Chevalier, C and Saillard, C and Bové, J M}, year={1990}, month=may, pages={2693–2703} }