Virulence genes promote conjugative transfer of the Ti plasmid between Agrobacterium strains
10.1128/jb.172.4.2191-2193.1990
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Certain virulence region operons of the Agrobacterium tumefaciens Ti plasmid promoted conjugative Ti plasmid transfer. Mutations in the vir region of pTiC58 inhibited conjugative plasmid transfer between A. tumefaciens strains. Mutations in virA, virG, 5' virB, and virE had the greatest effect on plasmid transfer, and mutations in virC had no effect. Transfer inhibition in vir mutants occurred in the presence or absence of acetosyringone.

Bibliography

Steck, T. R., & Kado, C. I. (1990). Virulence genes promote conjugative transfer of the Ti plasmid between Agrobacterium strains. Journal of Bacteriology, 172(4), 2191–2193.

Authors 2
  1. T R Steck (first)
  2. C I Kado (additional)
References 37 Referenced 28
  1. Albright , L. M. , M. F. Yanofsky , B. Leroux , D. Ma , and E. W. Nester . 1987 . Processing of the T-DNA of A. tumefaciens generates border nicks and linear, single-stranded T-DNA . J. Bacteriol. 169 : 1046 - 1055 . (10.1128/jb.169.3.1046-1055.1987) / J. Bacteriol. / Processing of the T-DNA of A. tumefaciens generates border nicks and linear, single-stranded T-DNA by Albright L. M. (1987)
  2. Bomhoff , G. , P. M. KlapwiJk , H. C. M. Kestler , R. A. Schilperoort , J. P. Hernalsteens , and J. Schell . 1976 . Octopine and nopaline synthesis and breakdown genetically controlled by a plasmid of Agrobacterium tumefaciens . Mol. Gen. Genet. 145 : 177 - 181 . (10.1007/BF00269591) / Mol. Gen. Genet. / Octopine and nopaline synthesis and breakdown genetically controlled by a plasmid of Agrobacterium tumefaciens by Bomhoff G. (1976)
  3. Buchanan-Wollaston , V. , J. E. Passiatore , and F. Cannon . 1987 . The mob and oriT mobilization functions of a bacterial plasmid promote its transfer to plants . Nature (London) 328 : 172 - 175 . (10.1038/328172a0) / Nature (London) / The mob and oriT mobilization functions of a bacterial plasmid promote its transfer to plants by Buchanan-Wollaston V. (1987)
  4. Chilton , W. S. , and M. -D. Chilton . 1984 . Mannityl opine analogs allow isolation of catabolic pathway regulatory mutants . J. Bacteriol. 158 : 650 - 658 . (10.1128/jb.158.2.650-658.1984) / J. Bacteriol. / Mannityl opine analogs allow isolation of catabolic pathway regulatory mutants by Chilton W. S. (1984)
  5. Christie , P. J. , J. E. Ward , S. Winans , and E. W. Nester . 1988 . The Agrobacterium tumefaciens virE2 gene product is a singlestranded-DNA-binding protein that associates with T-DNA . J. Bacteriol. 170 : 2659 - 2667 . (10.1128/jb.170.6.2659-2667.1988) / J. Bacteriol. / The Agrobacterium tumefaciens virE2 gene product is a singlestranded-DNA-binding protein that associates with T-DNA by Christie P. J. (1988)
  6. Citovsky , V. , G. DeVos , and P. Zambryski . 1988 . Singlestranded DNA binding protein encoded by the virE locus of Agrobacterium tumefaciens . Science 240 : 501 - 504 . (10.1126/science.240.4851.501) / Science / Singlestranded DNA binding protein encoded by the virE locus of Agrobacterium tumefaciens by Citovsky V. (1988)
  7. Das , A. 1988 . The A. tumefaciens virE operon encodes a single stranded DNA binding protein . Proc. Natl. Acad. Sci. USA 85 : 2509 - 2513 . / Proc. Natl. Acad. Sci. USA / The A. tumefaciens virE operon encodes a single stranded DNA binding protein by Das A. (1988)
  8. Depicker , A. , M. Dewilde , G. DeVos , R. DeVos , M. Van Montagu , and J. Schell . 1980 . Molecular cloning of overlapping segments of the nopaline Ti plasmid pTic 58 as a means to restriction endonuclease mapping . Plasmid 3 : 193 - 211 . (10.1016/0147-619X(80)90109-2) / Plasmid / Molecular cloning of overlapping segments of the nopaline Ti plasmid pTic 58 as a means to restriction endonuclease mapping by Depicker A. (1980)
  9. Ellis , J. G. , A. Kerr , and J. Tempe . 1982 . Conjugal transfer of nopaline and agropine Ti-plasmids-the role of agrocinopines . Mol. Gen. Genet. 186 : 269 - 274 . (10.1007/BF00331861) / Mol. Gen. Genet. / Conjugal transfer of nopaline and agropine Ti-plasmids-the role of agrocinopines by Ellis J. G. (1982)
  10. Ellis , J. G. , and P. J. Murphy . 1981 . Four new opines from crown gall tumours-their detection and properties . Mol. Gen. Genet. 181 : 36 - 43 . (10.1007/BF00339002) / Mol. Gen. Genet. / Four new opines from crown gall tumours-their detection and properties by Ellis J. G. (1981)
  11. Engstrom , P. , P. Zambryski , M. Van Montagu , and S. E. Stachel . 1987 . Characterization of Agrobacterium tumefaciens virulence proteins induced by the plant factor acetosyringone . J. Mol. Biol. 197 : 635 - 645 . (10.1016/0022-2836(87)90470-0) / J. Mol. Biol. / Characterization of Agrobacterium tumefaciens virulence proteins induced by the plant factor acetosyringone by Engstrom P. (1987)
  12. Firmin , J. L. , and G. R. Fenwick . 1978 . Agropine-a major new plasmid-determined metabolite in crown gall tumours . Nature (London) 276 : 842 - 844 . (10.1038/276842a0) / Nature (London) / Agropine-a major new plasmid-determined metabolite in crown gall tumours by Firmin J. L. (1978)
  13. Genetello , C. , N. Van Larebeke , M. Holsters , M. Van Montagu , and J. Scheli . 1977 . Ti plasmids of Agrobacterium as conjugative plasmids . Nature (London) 265 : 561 - 562 . (10.1038/265561a0) / Nature (London) / Ti plasmids of Agrobacterium as conjugative plasmids by Genetello C. (1977)
  14. Gietl , C. , Z. Koukolikova-Nicola , and B. Hohn . 1987 . Mobilization of T-DNA from Agrobacterium to plant cells involves a protein that binds single stranded DNA . Proc. Natl. Acad. Sci. USA 84 : 9006 - 9010 . (10.1073/pnas.84.24.9006) / Proc. Natl. Acad. Sci. USA / Mobilization of T-DNA from Agrobacterium to plant cells involves a protein that binds single stranded DNA by Gietl C. (1987)
  15. Hirooka , T. , and C. I. Kado . 1986 . Location of the right boundary of the virulence region on Agrobacterium tumefaciens plasmid pTiC58 and a host-specifying gene next to the boundary . J. Bacteriol. 168 : 237 - 243 . (10.1128/jb.168.1.237-243.1986) / J. Bacteriol. / Location of the right boundary of the virulence region on Agrobacterium tumefaciens plasmid pTiC58 and a host-specifying gene next to the boundary by Hirooka T. (1986)
  16. Holsters , M. , B. Silva , F. Van Vliet , C. Genetello , M. DeBlock , P. Dhaese , A. Depicker , D. Inze , G. Engler , R. Villarroel , M. Van Montagu , and J. Schell . 1980 . The functional organization of the nopaline A. tumefaciens plasmid pTiC58 . Plasmid 3 : 212 - 230 . (10.1016/0147-619X(80)90110-9) / Plasmid / The functional organization of the nopaline A. tumefaciens plasmid pTiC58 by Holsters M. (1980)
  17. Hooykaas , P. J. J. , P. M. Klapwik , M. P. Nuti , R. A. Schilperoort , and A. Rorsch . 1977 . Transfer of the Agrobacterium tumefaciens Ti plasmid to avirulent Agrobacteria and to Rhizobium ex planta . J. Gen. Microbiol. 98 : 477 - 484 . (10.1099/00221287-98-2-477) / J. Gen. Microbiol. / Transfer of the Agrobacterium tumefaciens Ti plasmid to avirulent Agrobacteria and to Rhizobium ex planta by Hooykaas P. J. J. (1977)
  18. Ippen-Ihler K. A. and E. G. Minkley Jr. 1986. The conjugation NOTES 2193
  19. Jayaswal , R. K. , K. Veluthambi , S. B. Gelvin , and J. L. Slightom . 1987 . Double-stranded cleavage of T-DNA and generation of single-stranded T-DNA molecules in Escherichia coli by a virD-encoded border-specific endonuclease from Agrobacterium tumefaciens . J. Bacteriol. 169 : 5035 - 5045 . (10.1128/jb.169.11.5035-5045.1987) / J. Bacteriol. / Double-stranded cleavage of T-DNA and generation of single-stranded T-DNA molecules in Escherichia coli by a virD-encoded border-specific endonuclease from Agrobacterium tumefaciens by Jayaswal R. K. (1987)
  20. Kao , J. C. , K. L. Perry , and C. I. Kado . 1982 . Indoleacetic acid complementation and its relation to host range specifying genes on the Ti plasmid of Agrobacterium tumefaciens . Mol. Gen. Genet. 188 : 425 - 432 . (10.1007/BF00330044) / Mol. Gen. Genet. / Indoleacetic acid complementation and its relation to host range specifying genes on the Ti plasmid of Agrobacterium tumefaciens by Kao J. C. (1982)
  21. Kerr , A. , P. Manigault , and J. Tempe . 1977 . Transfer of virulence in vivo and in vitro in Agrobacterium . Nature (London) 265 : 560 - 561 . (10.1038/265560a0) / Nature (London) / Transfer of virulence in vivo and in vitro in Agrobacterium by Kerr A. (1977)
  22. Klapwjk , P. M. , T. Scheulderman , and R. A. Schilperoort . 1978 . Coordinated regulation of octopine degradation and conjugative transfer of Ti plasmids in Agrobacterium tumefaciens: evidence for a common regulatory gene and separate operons . J. Bacteriol. 136 : 775 - 785 . (10.1128/jb.136.2.775-785.1978) / J. Bacteriol. / Coordinated regulation of octopine degradation and conjugative transfer of Ti plasmids in Agrobacterium tumefaciens: evidence for a common regulatory gene and separate operons by Klapwjk P. M. (1978)
  23. Klapwjk , P. M. , P. van Beelen , and R. A. Schilperoort . 1979 . Isolation of a recombinant deficient Agrobacterium tumefaciens mutant . Mol. Gen. Genet. 173 : 171 - 175 . (10.1007/BF00330307) / Mol. Gen. Genet. / Isolation of a recombinant deficient Agrobacterium tumefaciens mutant by Klapwjk P. M. (1979)
  24. Koekeman , B. P. , G. Ooms , P. M. Klapwik , and R. A. Schilperoort . 1979 . Genetic map of an octopine Ti-plasmid . Plasmid 2 : 347 - 357 . (10.1016/0147-619X(79)90018-0) / Plasmid / Genetic map of an octopine Ti-plasmid by Koekeman B. P. (1979)
  25. Koukolikova-Nicola Z. L. Albright and B. Hohn. 1987. The mechanism of T-DNA transfer from Agrobacterium tumefaciens to the plant cell p. 109-148. In T. Hohn and J. Schell (ed.) Plant DNA infectious agents. Springer-Verlag New York. (10.1007/978-3-7091-6977-3_5)
  26. Langley , R. A. , and C. I. Kado . 1972 . Studies on Agrobacterium tumefaciens: conditions for mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine and relationships of A. tumefaciens to crown-gall tumor induction . Mutat. Res. 14 : 277 - 286 . (10.1016/0027-5107(72)90014-0) / Mutat. Res. / Studies on Agrobacterium tumefaciens: conditions for mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine and relationships of A. tumefaciens to crown-gall tumor induction by Langley R. A. (1972)
  27. Lundquist , R. C. , T. J. Close , and C. I. Kado . 1984 . Genetic complementation of Agrobacterium tumefaciens Ti plasmid mutants in the virulence region . Mol. Gen. Genet. 193 : 1 - 7 . (10.1007/BF00327406) / Mol. Gen. Genet. / Genetic complementation of Agrobacterium tumefaciens Ti plasmid mutants in the virulence region by Lundquist R. C. (1984)
  28. Petit , A. , J. Tempe , A. Kerr , M. Holsters , M. Van Montagu , and J. Schell . 1978 . Substrate induction of conjugative activity of Agrobacterium tumefaciens Ti-plasmids . Nature (London) 271 : 570 - 571 . (10.1038/271570a0) / Nature (London) / Substrate induction of conjugative activity of Agrobacterium tumefaciens Ti-plasmids by Petit A. (1978)
  29. Rogowsky , P. M. , T. J. Close , J. A. Chimera , J. J. Shaw , and C. I. Kado . 1987 . Regulation of the vir genes of Agrobacterium tumefaciens plasmid pTiC58 . J. Bacteriol. 169 : 5101 - 5112 . (10.1128/jb.169.11.5101-5112.1987) / J. Bacteriol. / Regulation of the vir genes of Agrobacterium tumefaciens plasmid pTiC58 by Rogowsky P. M. (1987)
  30. Sprinzl , M. , and K. Geider . 1988 . Transfer of the Ti plasmid from Agrobacterium tumefaciens into Escherichia coli cells . J. Gen. Microbiol. 134 : 413 - 424 . / J. Gen. Microbiol. / Transfer of the Ti plasmid from Agrobacterium tumefaciens into Escherichia coli cells by Sprinzl M. (1988)
  31. Stachel , S. E. , B. Timmerman , and P. Zambryski . 1986 . Generation of single-stranded T-DNA molecules during the initial stages of T-DNA transfer from Agrobacterium tumefaciens to plant cells . Nature (London) 322 : 706 - 712 . (10.1038/322706a0) / Nature (London) / Generation of single-stranded T-DNA molecules during the initial stages of T-DNA transfer from Agrobacterium tumefaciens to plant cells by Stachel S. E. (1986)
  32. Stachel , S. E. , B. Timmerman , and P. Zambryski . 1987 . Activation of Agrobacterium tumefaciens vir gene expression generates multiple single-stranded T-strand molecules from the pTiA6 T-region: requirement for 5' virD gene products . EMBO J. 6 : 857 - 863 . (10.1002/j.1460-2075.1987.tb04831.x) / EMBO J. / Activation of Agrobacterium tumefaciens vir gene expression generates multiple single-stranded T-strand molecules from the pTiA6 T-region: requirement for 5' virD gene products by Stachel S. E. (1987)
  33. Stachel , S. E. , and P. Zambryski . 1986 . virA and virG control the plant-induced activation of the T-DNA transfer process of Agrobacterium tumefaciens . Cell 46 : 325 - 333 . (10.1016/0092-8674(86)90653-7) / Cell / virA and virG control the plant-induced activation of the T-DNA transfer process of Agrobacterium tumefaciens by Stachel S. E. (1986)
  34. Steck , T. R. , T. J. Close , and C. I. Kado . 1989 . High levels of double-stranded transferred DNA (T-DNA) from an intact nopaline Ti plasmid . Proc. Natl. Acad. Sci. USA 86 : 2133 - 2137 . (10.1073/pnas.86.7.2133) / Proc. Natl. Acad. Sci. USA / High levels of double-stranded transferred DNA (T-DNA) from an intact nopaline Ti plasmid by Steck T. R. (1989)
  35. Willetts , N. , and B. Wilkins . 1984 . Processing of plasmid DNA during bacterial conjugation . Microbiol. Rev. 48 : 24 - 41 . (10.1128/mr.48.1.24-41.1984) / Microbiol. Rev. / Processing of plasmid DNA during bacterial conjugation by Willetts N. (1984)
  36. Yanofsky , M. F. , S. G. Porter , C. Young , L. M. Albright , M. P. Gordon , and E. W. Nester . 1986 . The virD operon of Agrobacterium tumefaciens encodes a site-specific endonuclease . Cell 47 : 471 - 477 . (10.1016/0092-8674(86)90604-5) / Cell / The virD operon of Agrobacterium tumefaciens encodes a site-specific endonuclease by Yanofsky M. F. (1986)
  37. Zambryski , P. 1988 . Basic processes underlying Agrobacterium-mediated DNA transfer to plant cells. Annu . Rev. Genet. 22 : 1 - 30 . (10.1146/annurev.ge.22.120188.000245) / Rev. Genet. / Basic processes underlying Agrobacterium-mediated DNA transfer to plant cells. Annu by Zambryski P. (1988)
Dates
Type When
Created 8 years, 9 months ago (Nov. 14, 2016, 12:25 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:49 p.m.)
Indexed 3 months, 2 weeks ago (May 18, 2025, 10:25 a.m.)
Issued 35 years, 5 months ago (April 1, 1990)
Published 35 years, 5 months ago (April 1, 1990)
Published Print 35 years, 5 months ago (April 1, 1990)
Funders 0

None

@article{Steck_1990, title={Virulence genes promote conjugative transfer of the Ti plasmid between Agrobacterium strains}, volume={172}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.172.4.2191-2193.1990}, DOI={10.1128/jb.172.4.2191-2193.1990}, number={4}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Steck, T R and Kado, C I}, year={1990}, month=apr, pages={2191–2193} }