DOI: 10.1128/jb.172.2.610-618.1990. Chromosome engineering in Saccharomyces cerevisiae by using a site-specific recombination system of a yeast plasmid

Chromosome engineering in Saccharomyces cerevisiae by using a site-specific recombination system of a yeast plasmid

10.1128/jb.172.2.610-618.1990

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

We have developed an effective method to delete or invert a chromosomal segment and to create reciprocal recombination between two nonhomologous chromosomes in Saccharomyces cerevisiae, using the site-specific recombination system of pSR1, a circular cryptic DNA plasmid resembling 2 microns DNA of S. cerevisiae but originating from another yeast, Zygosaccharomyces rouxii. A 2.1-kilobase-pair DNA fragment bearing the specific recombination site on the inverted repeats of pSR1 was inserted at target sites on a single or two different chromosomes of S. cerevisiae by using integrative vectors. The cells were then transformed with a plasmid bearing the R gene of pSR1, which encodes the site-specific recombination enzyme and is placed downstream of the GAL1 promoter. When the transformants were cultivated in galactose medium, the recombination enzyme produced by expression of the R gene created the modified chromosome(s) by recombination between two specific recombination sites inserted on the chromosome(s).

Bibliography

Matsuzaki, H., Nakajima, R., Nishiyama, J., Araki, H., & Oshima, Y. (1990). Chromosome engineering in Saccharomyces cerevisiae by using a site-specific recombination system of a yeast plasmid. Journal of Bacteriology, 172(2), 610â618.

Authors 5

H Matsuzaki (first)
R Nakajima (additional)
J Nishiyama (additional)
H Araki (additional)
Y Oshima (additional)

References 36 Referenced 72

Araki , H. , A. Jearnpipatkul , H. Tatsumi , T. Sakurai , K. Ushio , T. Muta , and Y. Oshima . 1985 . Molecular and functional organization of yeast plasmid pSR1 . J. Mol. Biol. 182 : 191 - 203 . (10.1016/0022-2836(85)90338-9) / J. Mol. Biol. / Molecular and functional organization of yeast plasmid pSR1 by Araki H. (1985)
Botstein , D. , S. C. Falco , S. E. Stewart , M. Brennan , S. Scherer , D. T. Stinchcomb , K. Struhl , and R. W. Davis . 1979 . Sterile host yeasts (SHY): a eucaryotic system of biological containment for recombinant DNA experiments . Gene 8 : 17 - 24 . (10.1016/0378-1119(79)90004-0) / Gene / Sterile host yeasts (SHY): a eucaryotic system of biological containment for recombinant DNA experiments by Botstein D. (1979)
Broach J. R. 1981. The yeast plasmid 2i- circle p. 445-470. In J. N. Strathern E. W. Jones and J. R. Broach (ed.) The molecular biology of the yeast Saccharomyces: life cycle and inheritance. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
Broach , J. R. , Y. -Y. Li , J. Feldman , M. Jayaram , J. Abraham , K. A. Nasmyth , and J. B. Hicks . 1983 . Localization and sequence analysis of yeast origins of DNA replication . Cold Spring Harbor Symp. Quant. Biol. 47 : 1165 - 1173 . (10.1101/SQB.1983.047.01.132) / Cold Spring Harbor Symp. Quant. Biol. / Localization and sequence analysis of yeast origins of DNA replication by Broach J. R. (1983)
Broach J. R. Y.-Y. Li L.-C. C. Wu and M. Jayaram. 1983. Vectors for high-level inducible expression of cloned genes in yeast p. 83-117. In M. Inouye (ed.) Experimental manipulation of gene expression. Academic Press Inc. New York. (10.1016/B978-0-12-372380-2.50009-3)
Burke , D. T. , G. F. Carle , and M. V. Olson . 1987 . Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors . Science 236 : 806 - 812 . (10.1126/science.3033825) / Science / Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors by Burke D. T. (1987)
Carle , G. F. , and M. V. Olson . 1985 . An electrophoretic karyotype for yeast . Proc. Natl. Acad. Sci. USA 82 : 3756 - 3760 . (10.1073/pnas.82.11.3756) / Proc. Natl. Acad. Sci. USA / An electrophoretic karyotype for yeast by Carle G. F. (1985)
Chu , G. , D. Vollrath , and R. W. Davis . 1986 . Separation of large DNA molecules by contour-clamped homogeneous electric fields . Science 234 : 1582 - 1585 . (10.1126/science.3538420) / Science / Separation of large DNA molecules by contour-clamped homogeneous electric fields by Chu G. (1986)
Clarke , L. , and J. Carbon . 1978 . Functional expression of cloned yeast DNA in Escherichia coli: specific complementation of arginosuccinate Iyase (argH) mutations . J. Mol. Biol. 120 : 517 - 532 . (10.1016/0022-2836(78)90351-0) / J. Mol. Biol. / Functional expression of cloned yeast DNA in Escherichia coli: specific complementation of arginosuccinate Iyase (argH) mutations by Clarke L. (1978)
DeFeo-Jones , D. , E. M. Scolnick , R. Kolier , and R. Dhar . 1983 . ras-Related gene sequences identified and isolated from Saccharomyces cerevisiae . Nature (London) 306 : 707 - 709 . (10.1038/306707a0) / Nature (London) / ras-Related gene sequences identified and isolated from Saccharomyces cerevisiae by DeFeo-Jones D. (1983)
Denhardt D. T. 1%6. A membrane-filter technique for the detection of complementary DNA. Biochim. Biophys. Res. Commun. 23:641-646. (10.1016/0006-291X(66)90447-5)
Falco , S. C. , L. Yuyang , J. R. Broach , and D. Botstein . 1982 . Genetic properties of chromosomally integrated 2,u plasmid DNA in yeast . Cell 29 : 573 - 584 . (10.1016/0092-8674(82)90173-8) / Cell / Genetic properties of chromosomally integrated 2,u plasmid DNA in yeast by Falco S. C. (1982)
Feinberg , A. P. , and B. Vogelstein . 1983 . A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity . Anal. Biochem. 132 : 6 - 13 . (10.1016/0003-2697(83)90418-9) / Anal. Biochem. / A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity by Feinberg A. P. (1983)
Hieter , P. , C. Mann , M. Snyder , and R. W. Davis . 1985 . Mitotic stability of yeast chromosomes: a colony color assay that measures nondisjunction and chromosome loss . Cell 40 : 381 - 392 . (10.1016/0092-8674(85)90152-7) / Cell / Mitotic stability of yeast chromosomes: a colony color assay that measures nondisjunction and chromosome loss by Hieter P. (1985)
Ito , H. , Y. Fukuda , K. Murata , and A. Kimura . 1983 . Transformation of intact yeast cells treated with alkali cations . J. Bacteriol. 153 : 163 - 168 . (10.1128/jb.153.1.163-168.1983) / J. Bacteriol. / Transformation of intact yeast cells treated with alkali cations by Ito H. (1983)
Jearnpipatkul , A. , H. Araki , and Y. Oshlna . 1987 . Factors encoded by and affecting the holding stability of yeast plasmid pSR1 . Mol. Gen. Genet. 206 : 88 - 94 . (10.1007/BF00326541) / Mol. Gen. Genet. / Factors encoded by and affecting the holding stability of yeast plasmid pSR1 by Jearnpipatkul A. (1987)
Jinks-Robertson , S. , and T. D. Petes . 1986 . Chromosomal translocations generated by high-frequency meiotic recombination between repeated yeast genes . Genetics 114 : 731 - 752 . (10.1093/genetics/114.3.731) / Genetics / Chromosomal translocations generated by high-frequency meiotic recombination between repeated yeast genes by Jinks-Robertson S. (1986)
Johnston , M. , and R. W. Davis . 1984 . Sequences that regulate the divergent GALl-GAL1O promoter in Saccharomyces cerevisiae . Mol. Cell. Biol. 4 : 1440 1448. / Mol. Cell. Biol. / Sequences that regulate the divergent GALl-GAL1O promoter in Saccharomyces cerevisiae by Johnston M. (1984)
Matsuzaki , H. , H. Araki , and Y. Oshima . 1988 . Gene conversion associated with site-specific recombination in yeast plasmid pSR1 . Mol. Cell. Biol. 8 : 955 - 962 . / Mol. Cell. Biol. / Gene conversion associated with site-specific recombination in yeast plasmid pSR1 by Matsuzaki H. (1988)
Mortimer R. K. and D. Schild. 1981. Genetic mapping in Saccharomyces cerevisiae p. 11-26. In J. N. Strathern E. W. Jones and J. R. Broach (ed.) The molecular biology of the yeast Saccharomyces: life cycle and inheritance. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
Mortimer , R. K. , and D. Schild . 1985 . Genetic map of Saccharomyces cerevisiae, edition 9 . Microbiol. Rev. 49 : 181 - 212 . (10.1128/mr.49.3.181-213.1985) / Microbiol. Rev. / Genetic map of Saccharomyces cerevisiae, edition 9 by Mortimer R. K. (1985)
Murray , A. W. , and J. W. Szostak . 1986 . Construction and behavior of circularly permuted and telocentric chromosomes in Saccharomyces cerevisiae . Mol. Cell. Biol. 6 : 3166 - 3172 . / Mol. Cell. Biol. / Construction and behavior of circularly permuted and telocentric chromosomes in Saccharomyces cerevisiae by Murray A. W. (1986)
Orr-Weaver , T. L. , J. W. Szostak , and R. J. Rothstein . 1983 . Genetic applications of yeast transformation with linear and gapped plasmids . Methods Enzymol. 101 : 228 - 245 . (10.1016/0076-6879(83)01017-4) / Methods Enzymol. / Genetic applications of yeast transformation with linear and gapped plasmids by Orr-Weaver T. L. (1983)
Potier , S. , B. Winsor , and F. Lacroute . 1982 . Genetic selection for reciprocal translocation at chosen chromosomal sites in Saccharomyces cerevisiae . Mol. Cell. Biol. 2 : 1025 - 1032 . / Mol. Cell. Biol. / Genetic selection for reciprocal translocation at chosen chromosomal sites in Saccharomyces cerevisiae by Potier S. (1982)
Rose , M. , P. Grisafl , and D. Botstein . 1984 . Structure and function of the yeast URA3 gene: expression in Escherichia coli . Gene 29 : 113 - 124 . (10.1016/0378-1119(84)90172-0) / Gene / Structure and function of the yeast URA3 gene: expression in Escherichia coli by Rose M. (1984)
Rothstein , R. J. 1983 . One-step gene disruption in yeast . Methods Enzymol. 101 : 202 - 211 . (10.1016/0076-6879(83)01015-0) / Methods Enzymol. / One-step gene disruption in yeast by Rothstein R. J. (1983)
Sauer , B. 1987 . Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae . Mol. Cell. Biol. 7 : 2087 - 2096 . / Mol. Cell. Biol. / Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae by Sauer B. (1987)
Schwartz , D. C. , and C. R. Cantor . 1984 . Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis . Cell 37 : 67 - 75 . (10.1016/0092-8674(84)90301-5) / Cell / Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis by Schwartz D. C. (1984)
Sherman F. G. R. Fink and J. B. Hicks. 1986. Methods in yeast genetics. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
Southern , E. M. 1975 . Detection of specific sequences among DNA fragments separated by gel electrophoresis . J. Mol. Biol. 98 : 503 - 517 . (10.1016/S0022-2836(75)80083-0) / J. Mol. Biol. / Detection of specific sequences among DNA fragments separated by gel electrophoresis by Southern E. M. (1975)
Struhl , K. , and R. W. Davis . 1980 . A physical, genetic and transcriptional map of the cloned his 3 gene region of Saccharomyces cerevisiae . J. Mol. Biol. 136 : 309 - 332 . (10.1016/0022-2836(80)90376-9) / J. Mol. Biol. / A physical, genetic and transcriptional map of the cloned his 3 gene region of Saccharomyces cerevisiae by Struhl K. (1980)
Struhl , K. , D. T. Stinchcomb , S. Scherer , and R. W. Davis . 1979 . High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules . Proc. Natl. Acad. Sci. USA 76 : 1035 - 1039 . (10.1073/pnas.76.3.1035) / Proc. Natl. Acad. Sci. USA / High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules by Struhl K. (1979)
Sugawara , N. , and J. W. Szostak . 1983 . Recombination between sequences in nonhomologous positions . Proc. Natl. Acad. Sci. USA 80 : 5675 - 5679 . (10.1073/pnas.80.18.5675) / Proc. Natl. Acad. Sci. USA / Recombination between sequences in nonhomologous positions by Sugawara N. (1983)
Surosky , R. T. , and B. -K. Tye . 1985 . Construction of telocentric chromosomes in Saccharomyces cerevisiae . Proc. Natl. Acad. Sci. USA 82 : 2106 - 2110 . (10.1073/pnas.82.7.2106) / Proc. Natl. Acad. Sci. USA / Construction of telocentric chromosomes in Saccharomyces cerevisiae by Surosky R. T. (1985)
Toh -e, A. , S. Tada , and Y. Oshhma . 1982 . 2-,um DNA-like plasmids in the osmophilic haploid yeast Saccharomyces rouxii . J. Bacteriol. 151 : 1380 - 1390 . (10.1128/jb.151.3.1380-1390.1982) / J. Bacteriol. / 2-,um DNA-like plasmids in the osmophilic haploid yeast Saccharomyces rouxii by Toh A. (1982)
VoUrath , D. , R. W. Davis , C. Connelly , and P. Hieter . 1988 . Physical mapping of large DNA by chromosome fragmentation . Proc. Natl. Acad. Sci. USA 85 : 6027 - 6031 . (10.1073/pnas.85.16.6027) / Proc. Natl. Acad. Sci. USA / Physical mapping of large DNA by chromosome fragmentation by VoUrath D. (1988)

Dates

Type	When
Created	8 years, 9 months ago (Nov. 14, 2016, 12:11 p.m.)
Deposited	4 years ago (July 29, 2021, 1:12 p.m.)
Indexed	3 months ago (May 22, 2025, 3:31 a.m.)
Issued	35 years, 6 months ago (Feb. 1, 1990)
Published	35 years, 6 months ago (Feb. 1, 1990)
Published Print	35 years, 6 months ago (Feb. 1, 1990)

Funders 0

None

BibTeX

@article{Matsuzaki_1990, title={Chromosome engineering in Saccharomyces cerevisiae by using a site-specific recombination system of a yeast plasmid}, volume={172}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.172.2.610-618.1990}, DOI={10.1128/jb.172.2.610-618.1990}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Matsuzaki, H and Nakajima, R and Nishiyama, J and Araki, H and Oshima, Y}, year={1990}, month=feb, pages={610–618} }

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      "author": "Botstein D.",
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      "unstructured": "Broach , J. R. , Y. -Y. Li , J. Feldman , M. Jayaram , J. Abraham , K. A. Nasmyth , and J. B. Hicks . 1983 . Localization and sequence analysis of yeast origins of DNA replication . Cold Spring Harbor Symp. Quant. Biol. 47 : 1165 - 1173 .",
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      "DOI": "10.1007/BF00326541",
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      "volume": "206",
      "author": "Jearnpipatkul A.",
      "year": "1987",
      "unstructured": "Jearnpipatkul , A. , H. Araki , and Y. Oshlna . 1987 . Factors encoded by and affecting the holding stability of yeast plasmid pSR1 . Mol. Gen. Genet. 206 : 88 - 94 .",
      "journal-title": "Mol. Gen. Genet."
    },
    {
      "key": "p_17",
      "doi-asserted-by": "crossref",
      "first-page": "731",
      "DOI": "10.1093/genetics/114.3.731",
      "article-title": "Chromosomal translocations generated by high-frequency meiotic recombination between repeated yeast genes",
      "volume": "114",
      "author": "Jinks-Robertson S.",
      "year": "1986",
      "unstructured": "Jinks-Robertson , S. , and T. D. Petes . 1986 . Chromosomal translocations generated by high-frequency meiotic recombination between repeated yeast genes . Genetics 114 : 731 - 752 .",
      "journal-title": "Genetics"
    },
    {
      "key": "p_18",
      "first-page": "1440",
      "article-title": "Sequences that regulate the divergent GALl-GAL1O promoter in Saccharomyces cerevisiae",
      "volume": "4",
      "author": "Johnston M.",
      "year": "1984",
      "unstructured": "Johnston , M. , and R. W. Davis . 1984 . Sequences that regulate the divergent GALl-GAL1O promoter in Saccharomyces cerevisiae . Mol. Cell. Biol. 4 : 1440 1448.",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "p_19",
      "first-page": "955",
      "article-title": "Gene conversion associated with site-specific recombination in yeast plasmid pSR1",
      "volume": "8",
      "author": "Matsuzaki H.",
      "year": "1988",
      "unstructured": "Matsuzaki , H. , H. Araki , and Y. Oshima . 1988 . Gene conversion associated with site-specific recombination in yeast plasmid pSR1 . Mol. Cell. Biol. 8 : 955 - 962 .",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "p_20",
      "unstructured": "Mortimer R. K. and D. Schild. 1981. Genetic mapping in Saccharomyces cerevisiae p. 11-26. In J. N. Strathern E. W. Jones and J. R. Broach (ed.) The molecular biology of the yeast Saccharomyces: life cycle and inheritance. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y."
    },
    {
      "key": "p_21",
      "doi-asserted-by": "crossref",
      "first-page": "181",
      "DOI": "10.1128/mr.49.3.181-213.1985",
      "article-title": "Genetic map of Saccharomyces cerevisiae, edition 9",
      "volume": "49",
      "author": "Mortimer R. K.",
      "year": "1985",
      "unstructured": "Mortimer , R. K. , and D. Schild . 1985 . Genetic map of Saccharomyces cerevisiae, edition 9 . Microbiol. Rev. 49 : 181 - 212 .",
      "journal-title": "Microbiol. Rev."
    },
    {
      "key": "p_22",
      "first-page": "3166",
      "article-title": "Construction and behavior of circularly permuted and telocentric chromosomes in Saccharomyces cerevisiae",
      "volume": "6",
      "author": "Murray A. W.",
      "year": "1986",
      "unstructured": "Murray , A. W. , and J. W. Szostak . 1986 . Construction and behavior of circularly permuted and telocentric chromosomes in Saccharomyces cerevisiae . Mol. Cell. Biol. 6 : 3166 - 3172 .",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "p_23",
      "doi-asserted-by": "crossref",
      "first-page": "228",
      "DOI": "10.1016/0076-6879(83)01017-4",
      "article-title": "Genetic applications of yeast transformation with linear and gapped plasmids",
      "volume": "101",
      "author": "Orr-Weaver T. L.",
      "year": "1983",
      "unstructured": "Orr-Weaver , T. L. , J. W. Szostak , and R. J. Rothstein . 1983 . Genetic applications of yeast transformation with linear and gapped plasmids . Methods Enzymol. 101 : 228 - 245 .",
      "journal-title": "Methods Enzymol."
    },
    {
      "key": "p_24",
      "first-page": "1025",
      "article-title": "Genetic selection for reciprocal translocation at chosen chromosomal sites in Saccharomyces cerevisiae",
      "volume": "2",
      "author": "Potier S.",
      "year": "1982",
      "unstructured": "Potier , S. , B. Winsor , and F. Lacroute . 1982 . Genetic selection for reciprocal translocation at chosen chromosomal sites in Saccharomyces cerevisiae . Mol. Cell. Biol. 2 : 1025 - 1032 .",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "p_25",
      "doi-asserted-by": "crossref",
      "first-page": "113",
      "DOI": "10.1016/0378-1119(84)90172-0",
      "article-title": "Structure and function of the yeast URA3 gene: expression in Escherichia coli",
      "volume": "29",
      "author": "Rose M.",
      "year": "1984",
      "unstructured": "Rose , M. , P. Grisafl , and D. Botstein . 1984 . Structure and function of the yeast URA3 gene: expression in Escherichia coli . Gene 29 : 113 - 124 .",
      "journal-title": "Gene"
    },
    {
      "key": "p_26",
      "doi-asserted-by": "crossref",
      "first-page": "202",
      "DOI": "10.1016/0076-6879(83)01015-0",
      "article-title": "One-step gene disruption in yeast",
      "volume": "101",
      "author": "Rothstein R. J.",
      "year": "1983",
      "unstructured": "Rothstein , R. J. 1983 . One-step gene disruption in yeast . Methods Enzymol. 101 : 202 - 211 .",
      "journal-title": "Methods Enzymol."
    },
    {
      "key": "p_27",
      "first-page": "2087",
      "article-title": "Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae",
      "volume": "7",
      "author": "Sauer B.",
      "year": "1987",
      "unstructured": "Sauer , B. 1987 . Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae . Mol. Cell. Biol. 7 : 2087 - 2096 .",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "p_28",
      "doi-asserted-by": "crossref",
      "first-page": "67",
      "DOI": "10.1016/0092-8674(84)90301-5",
      "article-title": "Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis",
      "volume": "37",
      "author": "Schwartz D. C.",
      "year": "1984",
      "unstructured": "Schwartz , D. C. , and C. R. Cantor . 1984 . Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis . Cell 37 : 67 - 75 .",
      "journal-title": "Cell"
    },
    {
      "key": "p_29",
      "unstructured": "Sherman F. G. R. Fink and J. B. Hicks. 1986. Methods in yeast genetics. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y."
    },
    {
      "key": "p_30",
      "doi-asserted-by": "crossref",
      "first-page": "503",
      "DOI": "10.1016/S0022-2836(75)80083-0",
      "article-title": "Detection of specific sequences among DNA fragments separated by gel electrophoresis",
      "volume": "98",
      "author": "Southern E. M.",
      "year": "1975",
      "unstructured": "Southern , E. M. 1975 . Detection of specific sequences among DNA fragments separated by gel electrophoresis . J. Mol. Biol. 98 : 503 - 517 .",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "p_31",
      "doi-asserted-by": "crossref",
      "first-page": "309",
      "DOI": "10.1016/0022-2836(80)90376-9",
      "article-title": "A physical, genetic and transcriptional map of the cloned his 3 gene region of Saccharomyces cerevisiae",
      "volume": "136",
      "author": "Struhl K.",
      "year": "1980",
      "unstructured": "Struhl , K. , and R. W. Davis . 1980 . A physical, genetic and transcriptional map of the cloned his 3 gene region of Saccharomyces cerevisiae . J. Mol. Biol. 136 : 309 - 332 .",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "p_32",
      "doi-asserted-by": "crossref",
      "first-page": "1035",
      "DOI": "10.1073/pnas.76.3.1035",
      "article-title": "High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules",
      "volume": "76",
      "author": "Struhl K.",
      "year": "1979",
      "unstructured": "Struhl , K. , D. T. Stinchcomb , S. Scherer , and R. W. Davis . 1979 . High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules . Proc. Natl. Acad. Sci. USA 76 : 1035 - 1039 .",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "p_33",
      "doi-asserted-by": "crossref",
      "first-page": "5675",
      "DOI": "10.1073/pnas.80.18.5675",
      "article-title": "Recombination between sequences in nonhomologous positions",
      "volume": "80",
      "author": "Sugawara N.",
      "year": "1983",
      "unstructured": "Sugawara , N. , and J. W. Szostak . 1983 . Recombination between sequences in nonhomologous positions . Proc. Natl. Acad. Sci. USA 80 : 5675 - 5679 .",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "p_34",
      "doi-asserted-by": "crossref",
      "first-page": "2106",
      "DOI": "10.1073/pnas.82.7.2106",
      "article-title": "Construction of telocentric chromosomes in Saccharomyces cerevisiae",
      "volume": "82",
      "author": "Surosky R. T.",
      "year": "1985",
      "unstructured": "Surosky , R. T. , and B. -K. Tye . 1985 . Construction of telocentric chromosomes in Saccharomyces cerevisiae . Proc. Natl. Acad. Sci. USA 82 : 2106 - 2110 .",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "p_35",
      "doi-asserted-by": "crossref",
      "first-page": "1380",
      "DOI": "10.1128/jb.151.3.1380-1390.1982",
      "article-title": "2-,um DNA-like plasmids in the osmophilic haploid yeast Saccharomyces rouxii",
      "volume": "151",
      "author": "Toh A.",
      "year": "1982",
      "unstructured": "Toh -e, A. , S. Tada , and Y. Oshhma . 1982 . 2-,um DNA-like plasmids in the osmophilic haploid yeast Saccharomyces rouxii . J. Bacteriol. 151 : 1380 - 1390 .",
      "journal-title": "J. Bacteriol."
    },
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      "key": "p_36",
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      "first-page": "6027",
      "DOI": "10.1073/pnas.85.16.6027",
      "article-title": "Physical mapping of large DNA by chromosome fragmentation",
      "volume": "85",
      "author": "VoUrath D.",
      "year": "1988",
      "unstructured": "VoUrath , D. , R. W. Davis , C. Connelly , and P. Hieter . 1988 . Physical mapping of large DNA by chromosome fragmentation . Proc. Natl. Acad. Sci. USA 85 : 6027 - 6031 .",
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