An expression vector for the archaebacterium Haloferax volcanii
10.1128/jb.172.12.7104-7110.1990
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The recent development of an efficient transformation method and shuttle vectors for Haloferax volcanii has set the stage for rapid progress in archaebacterial molecular biology. We describe a shuttle-expression vector that can be selected for and maintained in either H. volcanii or Escherichia coli and permits the expression of cloned genes in H. volcanii. The vector, pWL204, was constructed by incorporating an H. volcanii tRNA(Lys) gene promoter into a derivative of the H. volcanii-E. coli shuttle vector pWL102. The vector has been used to express a modified, intron-containing, H. mediterranei tRNA(Trp) gene (tRNA(Trp)-O167). Transcription from the tRNA(Lys) gene promoter in vivo was detected by Northern (RNA) analysis with an oligonucleotide probe complementary to the unique intron sequence of tRNA(Trp)-O167. Dependence of transcription on the tRNA(Lys) promoter was demonstrated by the absence of transcription when the promoter sequence was deleted from the vector and by mapping the transcription initiation site by primer extension.

Bibliography

Nieuwlandt, D. T., & Daniels, C. J. (1990). An expression vector for the archaebacterium Haloferax volcanii. Journal of Bacteriology, 172(12), 7104–7110.

Authors 2
  1. D T Nieuwlandt (first)
  2. C J Daniels (additional)
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Dates
Type When
Created 8 years, 10 months ago (Nov. 4, 2016, 1:07 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:47 p.m.)
Indexed 1 year, 10 months ago (Oct. 28, 2023, 9:31 a.m.)
Issued 34 years, 9 months ago (Dec. 1, 1990)
Published 34 years, 9 months ago (Dec. 1, 1990)
Published Print 34 years, 9 months ago (Dec. 1, 1990)
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@article{Nieuwlandt_1990, title={An expression vector for the archaebacterium Haloferax volcanii}, volume={172}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.172.12.7104-7110.1990}, DOI={10.1128/jb.172.12.7104-7110.1990}, number={12}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Nieuwlandt, D T and Daniels, C J}, year={1990}, month=dec, pages={7104–7110} }