A novel mutation, cog, which results in production of a new porin protein (OmpG) of Escherichia coli K-12
10.1128/jb.171.8.4105-4111.1989
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

A mutant of Escherichia coli K-12 which produces a new outer membrane protein, OmpG, was isolated and genetically and biochemically characterized. The presence of OmpG allows growth on maltodextrins in the absence of the LamB maltoporin. The data obtained from in vivo growth and uptake experiments suggested that the presence of the OmpG protein results in an increase in outer membrane permeability for small hydrophilic compounds. In light of these findings, we suggest that OmpG is a porinlike protein. The mutation which results in the expression of OmpG has been termed cog (for control of OmpG) and mapped to 29 min on the E. coli chromosome. Diploid analysis shows that the mutant cog-192 allele is recessive for both the Dex+ and OmpG+ phenotypes. We propose that the cog mutation destroys a negative regulatory function and therefore derepresses ompG expression.

Bibliography

Misra, R., & Benson, S. A. (1989). A novel mutation, cog, which results in production of a new porin protein (OmpG) of Escherichia coli K-12. Journal of Bacteriology, 171(8), 4105–4111.

Authors 2
  1. R Misra (first)
  2. S A Benson (additional)
References 35 Referenced 41
  1. Bachmann B. J. 1987. Linkage map of Escherichia coli K-12 edition 7 p. 807-876. In F. C. Neidhardt J. L. Ingraham K. B. Low B. Magasanik M. Schaechter and H. E. Umbarger (ed.) Escherichia coli and Salmonella typhimurium: cellular and molecular biology vol. 1. American Society for Microbiology Washington D.C.
  2. Bassford , P. J. , Jr. , D. L. Diedrich , C. A. Schnaitman , and P. Reeves . 1977 . Outer membrane proteins of Escherichia coli. VI. Protein alternation in bacteriophage-resistant mutants . J. Bacteriol. 131 : 608 - 622 . (10.1128/JB.131.2.608-622.1977) / J. Bacteriol. / Outer membrane proteins of Escherichia coli. VI. Protein alternation in bacteriophage-resistant mutants by Bassford P. J. (1977)
  3. Benson , S. A. , J. L. Occi , and B. A. Sampson . 1988 . Mutations that alter the pore function of the OmpF porin of Escherichia coli K12 . J. Mol. Biol. 203 : 961 - 970 . (10.1016/0022-2836(88)90121-0) / J. Mol. Biol. / Mutations that alter the pore function of the OmpF porin of Escherichia coli K12 by Benson S. A. (1988)
  4. Benz , R. 1988 . Structure and function of porins from gramnegative bacteria. Annu . Rev. Microbiol. 42 : 359 - 393 . (10.1146/annurev.mi.42.100188.002043) / Rev. Microbiol. / Structure and function of porins from gramnegative bacteria. Annu by Benz R. (1988)
  5. Blasband , A. J. , W. R. Marcotte , Jr. , and C. A. Schnaitman . 1986 . Structure of the Ic and nmpC outer membrane porin protein genes of lamboid bacteriophage . J. Mol. Biol. 261 : 12723 - 12732 . / J. Mol. Biol. / Structure of the Ic and nmpC outer membrane porin protein genes of lamboid bacteriophage by Blasband A. J. (1986)
  6. Casadaban , M. J. 1976 . Transposition and fusion of the lac genes to selected promoter in Escherichia coli using bacteriophage lambda and Mu . J. Mol. Biol. 104 : 541 - 555 . (10.1016/0022-2836(76)90119-4) / J. Mol. Biol. / Transposition and fusion of the lac genes to selected promoter in Escherichia coli using bacteriophage lambda and Mu by Casadaban M. J. (1976)
  7. Emr , S. , and T. J. Silhavy . 1980 . Mutations affecting localization of Escherichia coli outer membrane protein, the bacteriophage lambda receptor . J. Mol. Biol. 141 : 63 - 90 . (10.1016/S0022-2836(80)80029-5) / J. Mol. Biol. / Mutations affecting localization of Escherichia coli outer membrane protein, the bacteriophage lambda receptor by Emr S. (1980)
  8. Feinstein , S. I. , and K. B. Low . 1982 . Zygotic induction of the rac locus can cause cell death in E. coli . Mol. Gen. Genet. 187 : 231 - 235 . (10.1007/BF00331122) / Mol. Gen. Genet. / Zygotic induction of the rac locus can cause cell death in E. coli by Feinstein S. I. (1982)
  9. Ferenci , T. , M. Schwentorat , S. Ullrich , and J. Vilmart . 1980 . Lambda receptor in the outer membrane of Escherichia coli as a binding protein for maltodextrins and starch polysaccharides . J. Bacteriol. 142 : 521 - 526 . (10.1128/JB.142.2.521-526.1980) / J. Bacteriol. / Lambda receptor in the outer membrane of Escherichia coli as a binding protein for maltodextrins and starch polysaccharides by Ferenci T. (1980)
  10. Fralick , J. , and D. Diedrich . 1982 . Studies on the expression of outer membrane protein 2 in Escherichia coli . Mol. Gen. Genet. 188 : 139 - 142 . (10.1007/BF00333008) / Mol. Gen. Genet. / Studies on the expression of outer membrane protein 2 in Escherichia coli by Fralick J. (1982)
  11. Hall , M. N. , and T. J. Silhavy . 1981 . The ompB locus and the regulation of the outer membrane porin proteins of Escherichia coli K-12 . J. Mol. Biol. 146 : 23 - 43 . (10.1016/0022-2836(81)90364-8) / J. Mol. Biol. / The ompB locus and the regulation of the outer membrane porin proteins of Escherichia coli K-12 by Hall M. N. (1981)
  12. Hancock , R. E. W. , and A. M. Carey . 1980 . Protein D1-a glucose-inducible, pore-forming protein from the outer membrane of Pseudomonas aeruginosa . FEMS Microbiol. Lett. 8 : 105 - 109 . / FEMS Microbiol. Lett. / Protein D1-a glucose-inducible, pore-forming protein from the outer membrane of Pseudomonas aeruginosa by Hancock R. E. W. (1980)
  13. Highton , P. J. , Y. Chang , W. R. Marcotte , Jr. , and C. A. Schnaitman . 1985 . Evidence that the outer membrane protein gene nmpC of Escherichia coli K-12 lies with the defective qsr' prophage . J. Bacteriol. 162 : 256 - 262 . (10.1128/JB.162.1.256-262.1985) / J. Bacteriol. / Evidence that the outer membrane protein gene nmpC of Escherichia coli K-12 lies with the defective qsr' prophage by Highton P. J. (1985)
  14. Ito , K. , T. Sato , and T. Yura . 1977 . Synthesis and assembly of the membrane proteins in E. coli . Cell 11 : 551 - 559 . (10.1016/0092-8674(77)90073-3) / Cell / Synthesis and assembly of the membrane proteins in E. coli by Ito K. (1977)
  15. Kaiser , K. , and N. E. Murray . 1979 . Physical characterization of the "Rac prophage " in E. coli. Mol. Gen. Genet. 175 : 159 - 174 . (10.1007/BF00425532) / E. coli. Mol. Gen. Genet. / Physical characterization of the "Rac prophage by Kaiser K. (1979)
  16. Lin E. C. 1987. Dissimilatory pathways for sugars polyols and carboxylates p. 244-284. In F. C. Neidhardt J. L. Ingraham K. B. Low B. Magasanik M. Schaechter and H. E. Umbarger (ed.) Escherichia coli and Salmonella typhimurium: cellular and molecular biology. vol. 1. American Society for Microbiology Washington D.C.
  17. Lugtenberg , B. , J. Mejers , R. Peters , P. van der Hoek , and L. van Alphen . 1975 . Electrophoretic resolution of the major outer membrane proteins of Escherichia coli K-12 into four bands . FEBS Lett. 58 : 254 - 258 . (10.1016/0014-5793(75)80272-9) / FEBS Lett. / Electrophoretic resolution of the major outer membrane proteins of Escherichia coli K-12 into four bands by Lugtenberg B. (1975)
  18. Lugtenberg , B. , and L. van Alphen . 1983 . Molecular architecture and functioning of the outer membrane of Escherichia coli . Biochim. Biophys. Acta 737 : 51 - 115 . (10.1016/0304-4157(83)90014-X) / Biochim. Biophys. Acta / Molecular architecture and functioning of the outer membrane of Escherichia coli by Lugtenberg B. (1983)
  19. Maier , C. , E. Bremer , A. Schmid , and R. Benz . 1987 . Pore forming activity of the Tsx protein of Escherichia coli. Demonstration of a nucleotide-specific binding site . J. Biol. Chem. 263 : 2493 - 2499 . (10.1016/S0021-9258(18)69233-6) / J. Biol. Chem. / Pore forming activity of the Tsx protein of Escherichia coli. Demonstration of a nucleotide-specific binding site by Maier C. (1987)
  20. Miller J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
  21. Misra , R. , and S. A. Benson . 1988 . Isolation and characterization of OmpC porin mutants with altered pore properties . J. Bacteriol. 170 : 528 - 533 . (10.1128/JB.170.2.528-533.1988) / J. Bacteriol. / Isolation and characterization of OmpC porin mutants with altered pore properties by Misra R. (1988)
  22. Misra , R. , and S. A. Benson . 1988 . Genetic identification of the pore domain of the OmpC porin of Escherichia coli K-12 . J. Bacteriol. 170 : 3611 - 3617 . (10.1128/JB.170.8.3611-3617.1988) / J. Bacteriol. / Genetic identification of the pore domain of the OmpC porin of Escherichia coli K-12 by Misra R. (1988)
  23. Misra R. B. A. Sampson J. L. Occi and S. A. Benson. 1987. Identification of mutants with altered membrane permeability p. 436-442. In P. Actor L. Daneo-Moore M. L. Higgins M. R. J. Salton and G. D. Shockman (ed.) Antibiotic inhibition of bacterial cell surface assembly and function. American Society for Microbiology Washington D.C.
  24. Morona , R. , and P. Reeves . 1982 . The tolC locus of Escherichia coli affects the expression of three major outer membrane proteins . J. Bacteriol. 150 : 1016 - 1023 . (10.1128/JB.150.3.1016-1023.1982) / J. Bacteriol. / The tolC locus of Escherichia coli affects the expression of three major outer membrane proteins by Morona R. (1982)
  25. Nakae , T. 1986 . Outer-membrane permeability of bacteria . Crit. Rev. Microbiol. 13 : 1 - 62 . (10.3109/10408418609108734) / Crit. Rev. Microbiol. / Outer-membrane permeability of bacteria by Nakae T. (1986)
  26. Nikaido , H. , and M. Vaara . 1985 . Molecular basis of bacterial outer membrane permeability . Microbiol. Rev. 49 : 1 - 32 . (10.1128/MR.49.1.1-32.1985) / Microbiol. Rev. / Molecular basis of bacterial outer membrane permeability by Nikaido H. (1985)
  27. Overbeeke , N. , and B. Lugtenberg . 1980 . Expression of outer membrane protein e of Escherichia coli K-12 by phosphate limitation . FEBS Lett. 112 : 229 - 232 . (10.1016/0014-5793(80)80186-4) / FEBS Lett. / Expression of outer membrane protein e of Escherichia coli K-12 by phosphate limitation by Overbeeke N. (1980)
  28. Pugsley , A. P. , and C. A. Schnaitman . 1978 . Identification of three genes controlling production of new outer membrane pore proteins in Escherichia coli K-12 . J. Bacteriol. 135 : 1118 - 1129 . (10.1128/JB.135.3.1118-1129.1978) / J. Bacteriol. / Identification of three genes controlling production of new outer membrane pore proteins in Escherichia coli K-12 by Pugsley A. P. (1978)
  29. Pugsley , A. P. , and C. A. Schnaitman . 1978 . Outer membrane proteins of Escherichia coli. VII. Evidence that bacteriophagedirected protein 2 functions as a pore . J. Bacteriol. 133 : 1181 - 1189 . (10.1128/JB.133.3.1181-1189.1978) / J. Bacteriol. / Outer membrane proteins of Escherichia coli. VII. Evidence that bacteriophagedirected protein 2 functions as a pore by Pugsley A. P. (1978)
  30. Reeves P. 1979. The genetics of outer membrane proteins p. 256-291. In M. Inouye (ed.) Bacterial outer membranes. John Wiley & Sons Inc. New York.
  31. Rosenbusch , J. P. 1974 . Characterization of the major outer membrane protein from Escherichia coli: regular arrangement on the peptidoglycan and unusual dodecyl sulfate binding . J. Biol. Chem. 249 : 8019 - 8029 . / J. Biol. Chem. / Characterization of the major outer membrane protein from Escherichia coli: regular arrangement on the peptidoglycan and unusual dodecyl sulfate binding by Rosenbusch J. P. (1974)
  32. Sanderson K. E. and J. A. Hurley. 1987. Linkage map of Salmonella typhimurium p. 877-918. In F. C.Neidhardt J. L. Ingraham K. B. Low B. Magasanik M. Schaechter and H. E. Umbarger (ed.) Escherichia coli and Salmonella typhimurium: cellular and molecular biology vol. 2. American Society for Microbiology Washington D.C.
  33. Schwartz M. 1987. The maltose regulon p. 1482-1502. In F. C. Neidhardt J. L. Ingraham K. B. Low B. Magasanik M. Schaechter and H. E. Umbarger (ed.) Escherichia coli and Salmonella typhimurium: cellular and molecular biology vol. 2. American Society for Microbiology Washington D.C.
  34. Silhavy T. J. M. L. Berman and L. W. Enquist. 1984. Experiments with gene fusions. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
  35. Tommassen , J. , and B. Lugtenberg . 1980 . Outer membrane protein e of Escherichia coli K-12 is co-regulated with alkaline phosphatase . J. Bacteriol. 143 : 151 - 157 . (10.1128/jb.143.1.151-157.1980) / J. Bacteriol. / Outer membrane protein e of Escherichia coli K-12 is co-regulated with alkaline phosphatase by Tommassen J. (1980)
Dates
Type When
Created 8 years, 9 months ago (Nov. 7, 2016, 11:37 a.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:45 p.m.)
Indexed 2 months ago (July 5, 2025, 12:46 a.m.)
Issued 36 years, 1 month ago (Aug. 1, 1989)
Published 36 years, 1 month ago (Aug. 1, 1989)
Published Print 36 years, 1 month ago (Aug. 1, 1989)
Funders 0

None

@article{Misra_1989, title={A novel mutation, cog, which results in production of a new porin protein (OmpG) of Escherichia coli K-12}, volume={171}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.171.8.4105-4111.1989}, DOI={10.1128/jb.171.8.4105-4111.1989}, number={8}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Misra, R and Benson, S A}, year={1989}, month=aug, pages={4105–4111} }