DOI: 10.1128/jb.171.5.2900-2902.1989. Role of metabolism in the chemotactic response of Rhodobacter sphaeroides to ammonia

Role of metabolism in the chemotactic response of Rhodobacter sphaeroides to ammonia

10.1128/jb.171.5.2900-2902.1989

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Rhodobacter sphaeroides only showed chemotaxis towards ammonia if grown under nitrogen-limited conditions. This chemotactic response was completely inhibited by the addition of methionine sulfoximine. There was no effect of methionine sulfoximine treatment on motility or taxis towards propionate, demonstrating that the effect is specific to ammonia taxis. It is known that methionine sulfoximine inhibits glutamine synthetase and hence blocks ammonia assimilation. Methionine sulfoximine does not inhibit ammonia transport in R. sphaeroides; therefore, these results suggest that limited metabolism via a specific pathway is required subsequent to transport to elicit a chemotactic response to ammonia. Bacteria grown on high ammonia show transport but no chemotactic response to ammonia, suggesting that the pathway of assimilation is important in eliciting a chemotactic response.

Bibliography

Poole, P. S., & Armitage, J. P. (1989). Role of metabolism in the chemotactic response of Rhodobacter sphaeroides to ammonia. Journal of Bacteriology, 171(5), 2900â2902.

Authors 2

P S Poole (first)
J P Armitage (additional)

References 14 Referenced 31

Armitage , J. P. , C. Ingham , and M. C. W. Evans . 1985 . Role of proton motive force in phototactic and aerotactic responses of Rhodopseudomonas sphaeroides . J. Bacteriol. 161 : 967 - 972 . (10.1128/JB.161.3.967-972.1985) / J. Bacteriol. / Role of proton motive force in phototactic and aerotactic responses of Rhodopseudomonas sphaeroides by Armitage J. P. (1985)
Armitage , J. P. , and R. M. Macnab . 1987 . Unidirectional, intermittent rotation of the flagellum of Rhodobacter sphaeroides . J. Bacteriol. 169 : 514 - 518 . (10.1128/JB.169.2.514-518.1987) / J. Bacteriol. / Unidirectional, intermittent rotation of the flagellum of Rhodobacter sphaeroides by Armitage J. P. (1987)
Berg , H. C. , and D. A. Brown . 1972 . Chemotaxis in Escherichia coli analyzed by three-dimensional tracking . Nature (London) 239 : 500 - 504 . (10.1038/239500a0) / Nature (London) / Chemotaxis in Escherichia coli analyzed by three-dimensional tracking by Berg H. C. (1972)
Boyd , A. , and M. Simon . 1982 . Bacterial chemotaxis. Annu . Rev. Physiol. 44 : 501 - 517 . (10.1146/annurev.ph.44.030182.002441) / Rev. Physiol. / Bacterial chemotaxis. Annu by Boyd A. (1982)
Brown , C. M. , and R. A. Herbert . 1977 . Ammonia assimilation in members of the rhodospirillaceae . FEMS Microbiol. Lett. 1 : 43 - 46 . (10.1111/j.1574-6968.1977.tb00576.x) / FEMS Microbiol. Lett. / Ammonia assimilation in members of the rhodospirillaceae by Brown C. M. (1977)
Cordts , M. L. , and J. Gibson . 1987 . Ammonium and methylammonium transport in Rhodobacter sphaeroides . J. Bacteriol. 169 : 1632 - 1638 . (10.1128/JB.169.4.1632-1638.1987) / J. Bacteriol. / Ammonium and methylammonium transport in Rhodobacter sphaeroides by Cordts M. L. (1987)
Fawcett , J. K. , and J. E. Scott . 1960 . A rapid and precise method for the determination of urea . J. Clin. Pathol. 13 : 156 - 159 . (10.1136/jcp.13.2.156) / J. Clin. Pathol. / A rapid and precise method for the determination of urea by Fawcett J. K. (1960)
Hazelbauer , G. L. , and S. Harayama . 1983 . Sensory transduction in bacterial chemotaxis . Int. Rev. Cytol. 81 : 33 - 70 . (10.1016/S0074-7696(08)62334-7) / Int. Rev. Cytol. / Sensory transduction in bacterial chemotaxis by Hazelbauer G. L. (1983)
Hess , J. F. , K. Oosawa , N. Kaplan , and M. I. Simon . 1988 . Phosphorylation of three proteins in the signaling pathway of bacterial chemotaxis . Cell 53 : 79 - 87 . (10.1016/0092-8674(88)90489-8) / Cell / Phosphorylation of three proteins in the signaling pathway of bacterial chemotaxis by Hess J. F. (1988)
Hess , J. F. , K. Oosawa , P. Matsumura , and M. I. Simon . 1987 . Protein phosphorylation is involved in bacterial chemotaxis . Proc. Natl. Acad. Sci. USA 84 : 7609 - 7613 . (10.1073/pnas.84.21.7609) / Proc. Natl. Acad. Sci. USA / Protein phosphorylation is involved in bacterial chemotaxis by Hess J. F. (1987)
Ingham , C. J. , and J. P. Armitage . 1987 . Involvement of transport in Rhodobacter sphaeroides chemotaxis . J. Bacteriol. 169 : 5801 - 5807 . (10.1128/JB.169.12.5801-5807.1987) / J. Bacteriol. / Involvement of transport in Rhodobacter sphaeroides chemotaxis by Ingham C. J. (1987)
Macnab , R. M. , and S. -I. Aizawa . 1984 . Bacterial motility and the bacterial flagellar motor. Annu . Rev. Biophys. Bioeng. 13 : 51 - 83 . (10.1146/annurev.bb.13.060184.000411) / Rev. Biophys. Bioeng. / Bacterial motility and the bacterial flagellar motor. Annu by Macnab R. M. (1984)
Poole , P. S. , and J. P. Armitage . 1988 . Motility response of Rhodobacter sphaeroides to chemotactic stimulation . J. Bacteriol. 170 : 5673 - 5679 . (10.1128/JB.170.12.5673-5679.1988) / J. Bacteriol. / Motility response of Rhodobacter sphaeroides to chemotactic stimulation by Poole P. S. (1988)
Sockett , R. E. , J. P. Armitage , and M. C. W. Evans . 1987 . Methylation-independent and methylation-dependent chemotaxis in Rhodobacter sphaeroides and Rhodospirillum rubrum . J. Bacteriol. 169 : 5808 - 5814 . (10.1128/JB.169.12.5808-5814.1987) / J. Bacteriol. / Methylation-independent and methylation-dependent chemotaxis in Rhodobacter sphaeroides and Rhodospirillum rubrum by Sockett R. E. (1987)

Dates

Type	When
Created	8 years, 10 months ago (Nov. 4, 2016, 1:48 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:43 p.m.)
Indexed	1 year, 7 months ago (Feb. 1, 2024, 1:57 p.m.)
Issued	36 years, 4 months ago (May 1, 1989)
Published	36 years, 4 months ago (May 1, 1989)
Published Print	36 years, 4 months ago (May 1, 1989)

Funders 0

None

BibTeX

@article{Poole_1989, title={Role of metabolism in the chemotactic response of Rhodobacter sphaeroides to ammonia}, volume={171}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.171.5.2900-2902.1989}, DOI={10.1128/jb.171.5.2900-2902.1989}, number={5}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Poole, P S and Armitage, J P}, year={1989}, month=may, pages={2900–2902} }

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