DOI: 10.1128/jb.171.2.1206-1210.1989. Nucleotide sequence and transcriptional analysis of the Streptomyces griseus gene (afsA) responsible for A-factor biosynthesis

Nucleotide sequence and transcriptional analysis of the Streptomyces griseus gene (afsA) responsible for A-factor biosynthesis

10.1128/jb.171.2.1206-1210.1989

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The nucleotide sequence of the Streptomyces griseus afsA gene, possibly encoding a key enzyme for A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) biosynthesis, was determined. The translational initiation codon was identified by introducing out-of-frame mutations at appropriate positions by oligonucleotide-directed mutagenesis. The afsA gene was thus found to code for a protein of 301 amino acid residues and 32.6 kilodaltons whose codon usage pattern was in agreement with the general tendency of Streptomyces genes with an extremely high guanine-plus-cytosine content. High-resolution S1 nuclease mapping indicated that the transcriptional start point was the A residue, the first position of the ATG translational initiation codon.

Bibliography

Horinouchi, S., Suzuki, H., Nishiyama, M., & Beppu, T. (1989). Nucleotide sequence and transcriptional analysis of the Streptomyces griseus gene (afsA) responsible for A-factor biosynthesis. Journal of Bacteriology, 171(2), 1206â1210.

Authors 4

S Horinouchi (first)
H Suzuki (additional)
M Nishiyama (additional)
T Beppu (additional)

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Dates

Type	When
Created	8 years, 8 months ago (Dec. 5, 2016, 1:30 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:43 p.m.)
Indexed	1 year, 10 months ago (Oct. 29, 2023, 8:41 a.m.)
Issued	36 years, 7 months ago (Feb. 1, 1989)
Published	36 years, 7 months ago (Feb. 1, 1989)
Published Print	36 years, 7 months ago (Feb. 1, 1989)

Funders 0

None

BibTeX

@article{Horinouchi_1989, title={Nucleotide sequence and transcriptional analysis of the Streptomyces griseus gene (afsA) responsible for A-factor biosynthesis}, volume={171}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.171.2.1206-1210.1989}, DOI={10.1128/jb.171.2.1206-1210.1989}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Horinouchi, S and Suzuki, H and Nishiyama, M and Beppu, T}, year={1989}, month=feb, pages={1206–1210} }

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