DOI: 10.1128/jb.171.12.6511-6516.1989. New mre genes mreC and mreD, responsible for formation of the rod shape of Escherichia coli cells

New mre genes mreC and mreD, responsible for formation of the rod shape of Escherichia coli cells

10.1128/jb.171.12.6511-6516.1989

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

New shape-determining genes in the mre cluster at 71 min on the Escherichia coli chromosome map, named mreC and mreD, were identified by complementation experiments using delta mre-678 mutant cells, which have a 5-kilobase-pair deletion encompassing the mre region, and by DNA sequencing. The delta mre-678 mutant cells required three genes, the previously reported mreB gene and the two new genes, to restore the normal rod shape of the cells and normal sensitivity of growth to mecillinam. The mreC gene is preceded by the mreB gene and by a 65-base-pair spacing sequence containing a palindrome sequence and a possible Shine-Dalgarno sequence. The deduced amino acid sequence of the MreC protein consists of 367 amino acid residues with a molecular weight of 39,530. The initiation codon of the mreD gene overlaps the termination codon of the mreC gene by one nucleotide residue. The deduced amino acid sequence of the MreD protein consists of 162 amino acid residues with a molecular weight of 18,755. In vitro, the coding frames of mreC and mreD produced proteins with Mrs of 40,000 and 15,000, respectively, as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Bibliography

Wachi, M., Doi, M., Okada, Y., & Matsuhashi, M. (1989). New mre genes mreC and mreD, responsible for formation of the rod shape of Escherichia coli cells. Journal of Bacteriology, 171(12), 6511â6516.

Authors 4

M Wachi (first)
M Doi (additional)
Y Okada (additional)
M Matsuhashi (additional)

References 17 Referenced 105

Bardwell , J. C. A. , and E. A. Craig . 1984 . Major heat shock gene of Drosophila and the Escherichia coli heat-inducible dnaK gene are homologous . Proc. Natl. Acad. Sci. USA 81 : 848 - 852 . (10.1073/pnas.81.3.848) / Proc. Natl. Acad. Sci. USA / Major heat shock gene of Drosophila and the Escherichia coli heat-inducible dnaK gene are homologous by Bardwell J. C. A. (1984)
Bienz , M. 1984 . Xenopus hsp 70 genes are constitutively expressed in injected oocytes . EMBO J. 3 : 2477 - 2483 . (10.1002/j.1460-2075.1984.tb02159.x) / EMBO J. / Xenopus hsp 70 genes are constitutively expressed in injected oocytes by Bienz M. (1984)
DeVries , J. K. , and G. Zubay . 1967 . DNA-directed peptide synthesis. II. The synthesis of the a-fragment of the enzyme 13-galactosidase . Proc. Natl. Acad. Sci. USA 57 : 1010 - 1012 . (10.1073/pnas.57.4.1010) / Proc. Natl. Acad. Sci. USA / DNA-directed peptide synthesis. II. The synthesis of the a-fragment of the enzyme 13-galactosidase by DeVries J. K. (1967)
Doi , M. , M. Wachi , F. Ishino , S. Tomioka , M. Ito , Y. Sakagami , A. Suzuki , and M. Matsuhashi . 1988 . Determinations of the DNA sequence of the mreB gene and of the gene products of the mre region that function in formation of the rod shape of Escherichia coli cells . J. Bacteriol. 170 : 4619 - 4624 . (10.1128/JB.170.10.4619-4624.1988) / J. Bacteriol. / Determinations of the DNA sequence of the mreB gene and of the gene products of the mre region that function in formation of the rod shape of Escherichia coli cells by Doi M. (1988)
Ehring , R. , K. Beyreuther , J. K. Wright , and P. Overath . 1980 . In vitro and in vivo products of E. coli lactose permease gene are identical . Nature (London) 283 : 537 - 540 . (10.1038/283537a0) / Nature (London) / In vitro and in vivo products of E. coli lactose permease gene are identical by Ehring R. (1980)
Hattori , M. , and Y. Sakaki . 1986 . Dideoxy sequencing method using denatured plasmid templates . Anal. Biochem. 152 : 232 - 238 . (10.1016/0003-2697(86)90403-3) / Anal. Biochem. / Dideoxy sequencing method using denatured plasmid templates by Hattori M. (1986)
Ingolia , T. D. , E. A. Craig , and B. J. McCarthy . 1980 . Sequence of three copies of the gene for the major Drosophila heat shock induced protein and their flanking regions . Cell 21 : 669 - 679 . (10.1016/0092-8674(80)90430-4) / Cell / Sequence of three copies of the gene for the major Drosophila heat shock induced protein and their flanking regions by Ingolia T. D. (1980)
Ishino , F. , W. Park , S. Tomioka , S. Tamaki , I. Takase , K. Kunugita , H. Matsuzawa , S. Asoh , T. Ohta , B. G. Spratt , and M. Matsuhashi . 1986 . Peptidoglycan synthetic activities in membranes of Escherichia coli caused by overproduction of penicillin-binding protein 2 and RodA protein . J. Biol. Chem. 261 : 7024 - 7031 . (10.1016/S0021-9258(19)62717-1) / J. Biol. Chem. / Peptidoglycan synthetic activities in membranes of Escherichia coli caused by overproduction of penicillin-binding protein 2 and RodA protein by Ishino F. (1986)
Robinson , A. C. , D. J. Kenan , G. F. Hatfull , N. F. Sullivan , R. Spiegelberg , and W. D. Donachie . 1984 . DNA sequence and transcriptional organization of essential cell division genes ftsQ andftsA of Escherichia coli: evidence for overlapping transcriptional units . J. Bacteriol. 160 : 546 - 555 . (10.1128/JB.160.2.546-555.1984) / J. Bacteriol. / DNA sequence and transcriptional organization of essential cell division genes ftsQ andftsA of Escherichia coli: evidence for overlapping transcriptional units by Robinson A. C. (1984)
Sanger , F. , S. Nicklen , and A. R. Coulson . 1977 . DNA sequencing with chain-terminating inhibitors . Proc. Natl. Acad. Sci. USA 74 : 5463 - 5467 . (10.1073/pnas.74.12.5463) / Proc. Natl. Acad. Sci. USA / DNA sequencing with chain-terminating inhibitors by Sanger F. (1977)
Stoker , N. G. , N. F. Fairweather , and B. G. Spratt . 1982 . Versatile low-copy-number plasmid vectors for cloning in Escherichia coli . Gene 18 : 335 - 341 . (10.1016/0378-1119(82)90172-X) / Gene / Versatile low-copy-number plasmid vectors for cloning in Escherichia coli by Stoker N. G. (1982)
Stoker , N. G. , J. M. Pratt , and B. G. Spratt . 1983 . Identification of the rodA gene product of Escherichia coli . J. Bacteriol. 155 : 854 - 859 . (10.1128/JB.155.2.854-859.1983) / J. Bacteriol. / Identification of the rodA gene product of Escherichia coli by Stoker N. G. (1983)
Takeshita , S. , M. Sato , M. Toba , W. Masahashi , and T. Hashimoto-Gotoh . 1987 . High-copy-number and low-copy-number plasmid vectors for lacZ a-complementation and chloramphenicol- or kanamycin-resistance selection . Gene 61 : 63 - 74 . (10.1016/0378-1119(87)90365-9) / Gene / High-copy-number and low-copy-number plasmid vectors for lacZ a-complementation and chloramphenicol- or kanamycin-resistance selection by Takeshita S. (1987)
Tamaki , S. , H. Matsuzawa , and M. Matsuhashi . 1980 . Cluster of mrdA and mrdB genes responsible for the rod shape and mecillinam sensitivity of Escherichia coli . J. Bacteriol. 141 : 52 - 57 . (10.1128/JB.141.1.52-57.1980) / J. Bacteriol. / Cluster of mrdA and mrdB genes responsible for the rod shape and mecillinam sensitivity of Escherichia coli by Tamaki S. (1980)
Wachi , M. , M. Doi , S. Tamaki , W. Park , S. Nakajima-Jijima , and M. Matsuhashi . 1987 . Mutant isolation and molecular cloning of mre genes, which determine cell shape, sensitivity to mecillinam, and amount of penicillin-binding proteins in Escherichia coli . J. Bacteriol. 169 : 4935 - 4940 . (10.1128/JB.169.11.4935-4940.1987) / J. Bacteriol. / Mutant isolation and molecular cloning of mre genes, which determine cell shape, sensitivity to mecillinam, and amount of penicillin-binding proteins in Escherichia coli by Wachi M. (1987)
Wachi , M. , and M. Matsuhashi . 1989 . Negative control of cell division by mreB, a gene that functions in determining the rod shape of Escherichia coli cells . J. Bacteriol. 171 : 3123 - 3127 . (10.1128/jb.171.6.3123-3127.1989) / J. Bacteriol. / Negative control of cell division by mreB, a gene that functions in determining the rod shape of Escherichia coli cells by Wachi M. (1989)
Westling-Haggstrom , B. , and S. Normark . 1975 . Genetic and physiological analysis of an envB spherelike mutant of Escherichia coli K-12 and characterization of its transductants . J. Bacteriol. 123 : 75 - 82 . (10.1128/JB.123.1.75-82.1975) / J. Bacteriol. / Genetic and physiological analysis of an envB spherelike mutant of Escherichia coli K-12 and characterization of its transductants by Westling-Haggstrom B. (1975)

Dates

Type	When
Created	8 years, 9 months ago (Nov. 14, 2016, 11:42 a.m.)
Deposited	4 years ago (July 29, 2021, 1:11 p.m.)
Indexed	1 year ago (Aug. 4, 2024, 12:35 p.m.)
Issued	35 years, 8 months ago (Dec. 1, 1989)
Published	35 years, 8 months ago (Dec. 1, 1989)
Published Print	35 years, 8 months ago (Dec. 1, 1989)

Funders 0

None

BibTeX

@article{Wachi_1989, title={New mre genes mreC and mreD, responsible for formation of the rod shape of Escherichia coli cells}, volume={171}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.171.12.6511-6516.1989}, DOI={10.1128/jb.171.12.6511-6516.1989}, number={12}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Wachi, M and Doi, M and Okada, Y and Matsuhashi, M}, year={1989}, month=dec, pages={6511–6516} }

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