DOI: 10.1128/jb.171.1.353-359.1989. virG, a plasmid-coded virulence gene of Shigella flexneri: identification of the virG protein and determination of the complete coding sequence

virG, a plasmid-coded virulence gene of Shigella flexneri: identification of the virG protein and determination of the complete coding sequence

10.1128/jb.171.1.353-359.1989

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

On the 230-kilobase-pair (kb) virulence plasmid of Shigella flexneri 2a strain YSH6000, at least seven separate genetic determinants have been identified. One of them, an approximately 4-kb region, virG, that is required for the Sereny reaction, was extensively studied to examine the role of the virG region. The phenotype of a VirG- mutant (M94) of YSH6000 in the cytoplasm of cultured MK cells was characterized by a kinetic study of the invading shigellae. The observed phenotype of M94 in the cytoplasm indicated that the virG locus is not required for multiplication of the invading shigellae, but is essential for their spread to adjacent cells. The DNA region necessary for the VirG function was localized to a 3.6-kb DNA sequence on the 230-kb plasmid. A 130-kilodalton polypeptide was confirmed to be the virG product. External labeling of bacteria with 125I indicated that the 130-kilodalton virG protein is exposed on the bacterial surface. The nucleotide sequence of 4,472 bp, which contains the functional virG gene and its own regulatory sequence, was determined, and a large open reading frame encoding 1,102 amino acid residues was identified.

Bibliography

Lett, M. C., Sasakawa, C., Okada, N., Sakai, T., Makino, S., Yamada, M., Komatsu, K., & Yoshikawa, M. (1989). virG, a plasmid-coded virulence gene of Shigella flexneri: identification of the virG protein and determination of the complete coding sequence. Journal of Bacteriology, 171(1), 353â359.

Authors 8

M C Lett (first)
C Sasakawa (additional)
N Okada (additional)
T Sakai (additional)
S Makino (additional)
M Yamada (additional)
K Komatsu (additional)
M Yoshikawa (additional)

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Dates

Type	When
Created	8 years, 9 months ago (Nov. 4, 2016, 1:33 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:42 p.m.)
Indexed	11 months, 2 weeks ago (Sept. 10, 2024, 9:49 a.m.)
Issued	36 years, 7 months ago (Jan. 1, 1989)
Published	36 years, 7 months ago (Jan. 1, 1989)
Published Print	36 years, 7 months ago (Jan. 1, 1989)

Funders 0

None

BibTeX

@article{Lett_1989, title={virG, a plasmid-coded virulence gene of Shigella flexneri: identification of the virG protein and determination of the complete coding sequence}, volume={171}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.171.1.353-359.1989}, DOI={10.1128/jb.171.1.353-359.1989}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Lett, M C and Sasakawa, C and Okada, N and Sakai, T and Makino, S and Yamada, M and Komatsu, K and Yoshikawa, M}, year={1989}, month=jan, pages={353–359} }

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