The REV1 gene of Saccharomyces cerevisiae: isolation, sequence, and functional analysis
10.1128/jb.171.1.230-237.1989
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The REV1 gene of Saccharomyces cerevisiae is required for normal induction of mutations by physical and chemical agents. We have determined the sequence of a 3,485-base-pair segment of DNA that complements the rev1-1 mutant. Gene disruption was used to confirm that this DNA contained the REV1 gene. The sequenced segment contains a single long open reading frame, which can encode a polypeptide of 985 amino acid residues. The REV1 transcript is 3.1 kilobase pairs in length. Frameshift mutations introduced into the open reading frame yielded a Rev-phenotype. A base substitution, encoding Gly-193 to Arg-193, was found in this open reading frame in rev1-1. Deletion mutants, lacking segments of the 5' region of REV1, had intermediate mutability relative to REV1 and rev1-1; a complete deletion exhibited lower mutability than rev1-1. REV1 is not an essential gene. An in-frame fusion of the 5' end of the REV1 open reading frame to the lacZ gene produced beta-galactosidase activity constitutively. The predicted REV1 protein is hydrophilic, with a predicted pI of 9.82. No homologies to RAD1, RAD2, RAD3, RAD7, or RAD10 proteins were noted. A 152-residue internal segment displayed 25% identity with UMUC protein.

Bibliography

Larimer, F. W., Perry, J. R., & Hardigree, A. A. (1989). The REV1 gene of Saccharomyces cerevisiae: isolation, sequence, and functional analysis. Journal of Bacteriology, 171(1), 230–237.

Authors 3
  1. F W Larimer (first)
  2. J R Perry (additional)
  3. A A Hardigree (additional)
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Dates
Type When
Created 8 years, 10 months ago (Nov. 4, 2016, 1:33 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:42 p.m.)
Indexed 1 year, 1 month ago (July 25, 2024, 1:09 p.m.)
Issued 36 years, 8 months ago (Jan. 1, 1989)
Published 36 years, 8 months ago (Jan. 1, 1989)
Published Print 36 years, 8 months ago (Jan. 1, 1989)
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@article{Larimer_1989, title={The REV1 gene of Saccharomyces cerevisiae: isolation, sequence, and functional analysis}, volume={171}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.171.1.230-237.1989}, DOI={10.1128/jb.171.1.230-237.1989}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Larimer, F W and Perry, J R and Hardigree, A A}, year={1989}, month=jan, pages={230–237} }