DOI: 10.1128/jb.170.9.3953-3960.1988. Role of the 25-, 27-, and 29-kilodalton flagellins in Caulobacter crescentus cell motility: method for construction of deletion and Tn5 insertion mutants by gene replacement

Role of the 25-, 27-, and 29-kilodalton flagellins in Caulobacter crescentus cell motility: method for construction of deletion and Tn5 insertion mutants by gene replacement

10.1128/jb.170.9.3953-3960.1988

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Caulobacter crescentus incorporates two distinct, but related proteins into the polar flagellar filament: a 27-kilodalton (kDa) flagellin is assembled proximal to the hook and a 25-kDa flagellin forms the distal end of the filament. These two proteins and a third, related flagellin protein of 29 kDa are encoded by three tandem genes (alpha-flagellin cluster) in the flaEY gene cluster (S.A. Minnich and A. Newton, Proc. Natl. Acad. Sci. USA 84: 1142-1146, 1987). Since point mutations in flagellin genes had not been isolated their requirement for flagellum function and fla gene expression was not known. To address these questions, we developed a gene replacement protocol that uses cloned flagellin genes mutagenized by either Tn5 transposons in vivo or the replacement of specific DNA fragments in vitro by the antibiotic resistance omega cassette. Analysis of gene replacement mutants constructed by this procedure led to several conclusions. (i) Mutations in any of the three flagellin genes do not cause complete loss of motility. (ii) Tn5 insertions in the 27-kDa flagellin gene and a deletion mutant of this gene do not synthesize the 27-kDa flagellin, but they do synthesize wild-type levels of the 25-kDa flagellin, which implies that the 27-kDa flagellin is not required for expression and assembly of the 25-kDa flagellin; these mutants show slightly impaired motility on swarm plates. (iii) Mutant PC7810, which is deleted for the three flagellin genes in the flaEY cluster, does not synthesize the 27- or 29-kDa flagellin, and it is significantly more impaired for motility on swarm plates than mutants with defects in only the 27-kDa flagellin gene. The synthesis of essentially normal levels of 25-kDa flagellin by strain PC7810 confirms that additional copies of the 25-kDa flagellin map outside the flaEY cluster (beta-flagellin cluster) and that these flagellin genes are active. Thus, while the 29- and 27-kDa flagellins are not absolutely essential for motility in C. crescentus, their assembly into the flagellar structure is necessary for normal flagellar function.

Bibliography

Minnich, S. A., Ohta, N., Taylor, N., & Newton, A. (1988). Role of the 25-, 27-, and 29-kilodalton flagellins in Caulobacter crescentus cell motility: method for construction of deletion and Tn5 insertion mutants by gene replacement. Journal of Bacteriology, 170(9), 3953â3960.

Authors 4

S A Minnich (first)
N Ohta (additional)
N Taylor (additional)
A Newton (additional)

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Dates

Type	When
Created	8 years, 9 months ago (Nov. 3, 2016, 9:01 p.m.)
Deposited	4 years ago (July 29, 2021, 1:40 p.m.)
Indexed	1 year, 10 months ago (Sept. 28, 2023, 3:44 p.m.)
Issued	36 years, 11 months ago (Sept. 1, 1988)
Published	36 years, 11 months ago (Sept. 1, 1988)
Published Print	36 years, 11 months ago (Sept. 1, 1988)

Funders 0

None

BibTeX

@article{Minnich_1988, title={Role of the 25-, 27-, and 29-kilodalton flagellins in Caulobacter crescentus cell motility: method for construction of deletion and Tn5 insertion mutants by gene replacement}, volume={170}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.170.9.3953-3960.1988}, DOI={10.1128/jb.170.9.3953-3960.1988}, number={9}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Minnich, S A and Ohta, N and Taylor, N and Newton, A}, year={1988}, month=sep, pages={3953–3960} }

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