DOI: 10.1128/jb.169.8.3743-3749.1987. Evidence for multiple K+ export systems in Escherichia coli

Evidence for multiple K+ export systems in Escherichia coli

10.1128/jb.169.8.3743-3749.1987

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The role of the K+ transport systems encoded by the kefB (formerly trkB) and kefC (formerly trkC) genes of Escherichia coli in K+ efflux has been investigated. The rate of efflux produced by N-ethylmaleimide (NEM), increased turgor pressure, alkalinization of the cytoplasm, or 2,4-dinitrophenol in a mutant with null mutations in both kef genes was compared with the rate of efflux in a wild-type strain for kef. The results show that these two genes encode the major paths for NEM-stimulated efflux. However, neither efflux system appears to be a significant path of K+ efflux produced by high turgor pressure, by alkalinization of the cytoplasm, or by addition of high concentrations of 2,4-dinitrophenol. Therefore, this species must have at least one other system, besides those encoded by kefB and kefC, capable of mediating a high rate of K+ efflux. The high, spontaneous rate of K+ efflux characteristic of the kefC121 mutation increases further when the strain is treated with NEM. Therefore, the mutational defect that leads to spontaneous efflux in this strain does not abolish the site(s) responsible for the action of NEM.

Bibliography

Bakker, E. P., Booth, I. R., Dinnbier, U., Epstein, W., & Gajewska, A. (1987). Evidence for multiple K+ export systems in Escherichia coli. Journal of Bacteriology, 169(8), 3743â3749.

Authors 5

E P Bakker (first)
I R Booth (additional)
U Dinnbier (additional)
W Epstein (additional)
A Gajewska (additional)

References 30 Referenced 92

Bakker , E. P. 1983 . pH dependent transport of rubidium by the constitutive potassium uptake system TrkA of Escherichia coli . FEMS Microbiol. Lett. 16 : 229 - 233 . (10.1111/j.1574-6968.1983.tb00293.x) / FEMS Microbiol. Lett. / pH dependent transport of rubidium by the constitutive potassium uptake system TrkA of Escherichia coli by Bakker E. P. (1983)
Bakker , E. P. , and W. E. Mangerich . 1981 . Interconversion of components of the bacterial proton motive force by electrogenic potassium transport . J. Bacteriol. 147 : 820 - 826 . (10.1128/jb.147.3.820-826.1981) / J. Bacteriol. / Interconversion of components of the bacterial proton motive force by electrogenic potassium transport by Bakker E. P. (1981)
Bakker , E. P. , and W. E. Mangerich . 1982 . N-Ethylmaleimide induces K+-H+ antiport activity in Escherichia coli K-12 . FEBS Lett. 140 : 177 - 180 . (10.1016/0014-5793(82)80888-0) / FEBS Lett. / N-Ethylmaleimide induces K+-H+ antiport activity in Escherichia coli K-12 by Bakker E. P. (1982)
Booth , I. R. 1985 . Regulation of cytoplasmic pH in bacteria . Microbiol. Rev. 49 : 359 - 378 . (10.1128/mr.49.4.359-378.1985) / Microbiol. Rev. / Regulation of cytoplasmic pH in bacteria by Booth I. R. (1985)
Booth , I. R. , W. Epstein , P. M. Giffard , and G. C. Rowland . 1985 . Roles of the trkB and trkC gene products of Escherichia coli in K+ transport . Biochimie 67 : 83 - 90 . (10.1016/S0300-9084(85)80233-9) / Biochimie / Roles of the trkB and trkC gene products of Escherichia coli in K+ transport by Booth I. R. (1985)
Bremer , E. , T. J. Silhavy , and G. M. Weinstock . 1985 . Transposable A placMu bacteriophages for creating lacZ operon fusions and kanamycin resistance insertions in Escherichia coli . J. Bacteriol. 162 : 1092 - 1099 . (10.1128/jb.162.3.1092-1099.1985) / J. Bacteriol. / Transposable A placMu bacteriophages for creating lacZ operon fusions and kanamycin resistance insertions in Escherichia coli by Bremer E. (1985)
Brey , R. N. , J. C. Beck , and B. P. Rosen . 1978 . Cation/proton antiport systems in Escherichia coli . Biochem. Biophys. Res. Commun. 83 : 1588 - 1594 . (10.1016/0006-291X(78)91403-1) / Biochem. Biophys. Res. Commun. / Cation/proton antiport systems in Escherichia coli by Brey R. N. (1978)
Epstein , W. , and B. S. Kim . 1981 . Potassium transport loci in Escherichia coli . J. Bacteriol. 108 : 836 - 843 . / J. Bacteriol. / Potassium transport loci in Escherichia coli by Epstein W. (1981)
Epstein , W. , and S. G. Schultz . 1965 . Cation transport in Escherichia coli K-12. V. Regulation of cation content . J. Gen. Physiol. 49 : 221 - 234 . (10.1085/jgp.49.2.221) / J. Gen. Physiol. / Cation transport in Escherichia coli K-12. V. Regulation of cation content by Epstein W. (1965)
Epstein W. and S. G. Schultz. 1968. Ion transport and osmoregulation in bacteria p. 186-197. In L. B. Guze (ed.) Microbial protoplasts spheroplasts and L-forms. The Williams and Wilkins Co. Baltimore.
Harold , F. M. , and K. Altendorf . 1974 . Cation transport in bacteria: K', Na+ and H+ . Curr. Top. Membr. Transp. 5 : 1 - 50 . (10.1016/S0070-2161(08)60183-5) / Curr. Top. Membr. Transp. / Cation transport in bacteria: K', Na+ and H+ by Harold F. M. (1974)
Helmer G. L. L. A. Laimins and W. Epstein. 1982. Mechanisms of potassium transport in bacteria vol. 2 p. 123-128. In A. N. Martinosi (ed.) Membranes and transport. Plenum Publishing Corp. New York. (10.1007/978-1-4684-4085-0_19)
Itikawa , H. , S. Baumberg , and H. J. Vogel . 1968 . Enzymic basis for a genetic suppression: accumulation and deacylation of N-acetylglutamic y-semialdehyde in enterobacterial mutants . Biochim. Biophys. Acta 159 : 547 - 550 . (10.1016/0005-2744(68)90142-3) / Biochim. Biophys. Acta / Enzymic basis for a genetic suppression: accumulation and deacylation of N-acetylglutamic y-semialdehyde in enterobacterial mutants by Itikawa H. (1968)
Laimins , L. A. , D. B. Rhoads , and W. Epstein . 1981 . Osmotic control of kdp operon expression in Escherichia coli . Proc. Natl. Acad. Sci. USA 78 : 464 - 468 . (10.1073/pnas.78.1.464) / Proc. Natl. Acad. Sci. USA / Osmotic control of kdp operon expression in Escherichia coli by Laimins L. A. (1981)
Larsen , P. I. , L. K. Sydnes , B. Landfald , and A. R. Str 0m. 1987 . Osmoregulation in Escherichia coli by accumulation of organic osmolytes: betaines, glutamic acid, and trehalose . Arch. Microbiol. 147 : 1 - 7 . (10.1007/BF00492896) / Arch. Microbiol. / Osmoregulation in Escherichia coli by accumulation of organic osmolytes: betaines, glutamic acid, and trehalose by Larsen P. I. (1987)
Meury , J. , and A. Kepes . 1981 . The regulation of potassium fluxes for the adjustment and maintenance of potassium levels in Escherichia coli . Eur. J. Biochem. 119 : 165 - 170 . (10.1111/j.1432-1033.1981.tb05589.x) / Eur. J. Biochem. / The regulation of potassium fluxes for the adjustment and maintenance of potassium levels in Escherichia coli by Meury J. (1981)
Meury , J. , and A. Kepes . 1982 . Glutathione and the gated potassium channels of Escherichia coli . EMBO J. 1 : 339 - 343 . (10.1002/j.1460-2075.1982.tb01171.x) / EMBO J. / Glutathione and the gated potassium channels of Escherichia coli by Meury J. (1982)
Meury , J. , S. Lebail , and A. Kepes . 1980 . Opening of potassium channels in Escherichia coli membranes by thiol reagents and recovery of potassium tightness . Eur. J. Biochem. 113 : 33 - 38 . (10.1111/j.1432-1033.1980.tb06135.x) / Eur. J. Biochem. / Opening of potassium channels in Escherichia coli membranes by thiol reagents and recovery of potassium tightness by Meury J. (1980)
Meury , J. , A. Robin , and P. Monnier-Champeix . 1985 . Turgorcontrolled K' fluxes and their pathways in Escherichia coli . Eur. J. Biochem. 151 : 613 - 619 . (10.1111/j.1432-1033.1985.tb09148.x) / Eur. J. Biochem. / Turgorcontrolled K' fluxes and their pathways in Escherichia coli by Meury J. (1985)
Nakamura , T. , H. Tokuda , and T. Unemoto . 1982 . Effects of pH and monovalent cations on the potassium ion exit from the marine bacterium Vibrio alginolyticus and the manipulation of cellular cation content . Biochim. Biophys. Acta 692 : 389 - 396 . (10.1016/0005-2736(82)90389-3) / Biochim. Biophys. Acta / Effects of pH and monovalent cations on the potassium ion exit from the marine bacterium Vibrio alginolyticus and the manipulation of cellular cation content by Nakamura T. (1982)
Padan , E. , D. Zilberstein , and H. Rottenberg . 1976 . The proton electrochemical gradient in Escherichia coli cells . Eur. J. Biochem. 63 : 330 - 336 . (10.1111/j.1432-1033.1976.tb10257.x) / Eur. J. Biochem. / The proton electrochemical gradient in Escherichia coli cells by Padan E. (1976)
Plack , R. J. , Jr. , and B. P. Rosen . 1980 . Cation/proton antiport systems in Escherichia coli: absence of potassium/proton antiport activity in a pH-sensitive mutant . J. Biol. Chem. 255 : 3824 - 3825 . (10.1016/S0021-9258(19)85594-1) / J. Biol. Chem. / Cation/proton antiport systems in Escherichia coli: absence of potassium/proton antiport activity in a pH-sensitive mutant by Plack R. J. (1980)
Reed , R. H. , and A. E. Walsby . 1985 . Changes in turgor pressure in response to increases in external NaCI concentration in the gas-vacuolate cyanobacterium Microcystis sp . Arch. Microbiol. 143 : 290 - 296 . (10.1007/BF00411252) / Arch. Microbiol. / Changes in turgor pressure in response to increases in external NaCI concentration in the gas-vacuolate cyanobacterium Microcystis sp by Reed R. H. (1985)
Rhoads , D. B. , and W. Epstein . 1978 . Cation transport in Escherichia coli. IX. Regulation of K+ transport . J. Gen. Physiol. 72 : 283 - 295 . (10.1085/jgp.72.3.283) / J. Gen. Physiol. / Cation transport in Escherichia coli. IX. Regulation of K+ transport by Rhoads D. B. (1978)
Rhoads , D. B. , F. B. Waters , and W. Epstein . 1976 . Cation transport in Escherichia coli. VIII. Potassium transport mutants . J. Gen. Physiol. 67 : 325 - 341 . (10.1085/jgp.67.3.325) / J. Gen. Physiol. / Cation transport in Escherichia coli. VIII. Potassium transport mutants by Rhoads D. B. (1976)
Rottenberg , H. 1979 . Measurement of membrane potential in bacteria . Methods Enzymol. 55 : 547 - 569 . / Methods Enzymol. / Measurement of membrane potential in bacteria by Rottenberg H. (1979)
Silver S. 1978. Transport of cations and anions p. 221-234. In B. P. Rosen (ed.) Bacterial transport. Marcel Dekker Inc. New York.
Steinbach H. R. 1962. Comparative biochemistry of the alkali metals vol. 4 p. 677-720. In M. Florkin and H. Mason (ed.) Comparative biochemistry. Academic Press Inc. New York. (10.1016/B978-0-12-395545-6.50022-5)
Strom , A. R. , P. Falkenberg , and B. Landfald . 1986 . Genetics of osmoregulation in Escherichia coli: uptake and biosynthesis of organic osmolytes . FEMS Microbiol. Rev. 37 : 79 - 86 . (10.1111/j.1574-6968.1986.tb01846.x) / FEMS Microbiol. Rev. / Genetics of osmoregulation in Escherichia coli: uptake and biosynthesis of organic osmolytes by Strom A. R. (1986)
West , I. C. , and T. H. Wilson . 1973 . Galactoside transport dissociated from proton movements in mutants of Escherichia coli . Biochem. Biophys. Res. Commun. 50 : 551 - 558 . (10.1016/0006-291X(73)90875-9) / Biochem. Biophys. Res. Commun. / Galactoside transport dissociated from proton movements in mutants of Escherichia coli by West I. C. (1973)

Dates

Type	When
Created	8 years, 10 months ago (Nov. 2, 2016, 7:12 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:38 p.m.)
Indexed	1 year, 3 months ago (June 4, 2024, 12:35 p.m.)
Issued	38 years, 1 month ago (Aug. 1, 1987)
Published	38 years, 1 month ago (Aug. 1, 1987)
Published Print	38 years, 1 month ago (Aug. 1, 1987)

Funders 0

None

BibTeX

@article{Bakker_1987, title={Evidence for multiple K+ export systems in Escherichia coli}, volume={169}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.169.8.3743-3749.1987}, DOI={10.1128/jb.169.8.3743-3749.1987}, number={8}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Bakker, E P and Booth, I R and Dinnbier, U and Epstein, W and Gajewska, A}, year={1987}, month=aug, pages={3743–3749} }

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      "DOI": "10.1128/mr.49.4.359-378.1985",
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      "unstructured": "Booth , I. R. 1985 . Regulation of cytoplasmic pH in bacteria . Microbiol. Rev. 49 : 359 - 378 .",
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      "article-title": "Roles of the trkB and trkC gene products of Escherichia coli in K+ transport",
      "volume": "67",
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      "year": "1985",
      "unstructured": "Booth , I. R. , W. Epstein , P. M. Giffard , and G. C. Rowland . 1985 . Roles of the trkB and trkC gene products of Escherichia coli in K+ transport . Biochimie 67 : 83 - 90 .",
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      "year": "1982",
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      "volume": "113",
      "author": "Meury J.",
      "year": "1980",
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      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "p_19",
      "doi-asserted-by": "crossref",
      "first-page": "613",
      "DOI": "10.1111/j.1432-1033.1985.tb09148.x",
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      "volume": "151",
      "author": "Meury J.",
      "year": "1985",
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      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "p_20",
      "doi-asserted-by": "crossref",
      "first-page": "389",
      "DOI": "10.1016/0005-2736(82)90389-3",
      "article-title": "Effects of pH and monovalent cations on the potassium ion exit from the marine bacterium Vibrio alginolyticus and the manipulation of cellular cation content",
      "volume": "692",
      "author": "Nakamura T.",
      "year": "1982",
      "unstructured": "Nakamura , T. , H. Tokuda , and T. Unemoto . 1982 . Effects of pH and monovalent cations on the potassium ion exit from the marine bacterium Vibrio alginolyticus and the manipulation of cellular cation content . Biochim. Biophys. Acta 692 : 389 - 396 .",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "p_21",
      "doi-asserted-by": "crossref",
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      "DOI": "10.1111/j.1432-1033.1976.tb10257.x",
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      "volume": "63",
      "author": "Padan E.",
      "year": "1976",
      "unstructured": "Padan , E. , D. Zilberstein , and H. Rottenberg . 1976 . The proton electrochemical gradient in Escherichia coli cells . Eur. J. Biochem. 63 : 330 - 336 .",
      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "p_22",
      "doi-asserted-by": "crossref",
      "first-page": "3824",
      "DOI": "10.1016/S0021-9258(19)85594-1",
      "article-title": "Cation/proton antiport systems in Escherichia coli: absence of potassium/proton antiport activity in a pH-sensitive mutant",
      "volume": "255",
      "author": "Plack R. J.",
      "year": "1980",
      "unstructured": "Plack , R. J. , Jr. , and B. P. Rosen . 1980 . Cation/proton antiport systems in Escherichia coli: absence of potassium/proton antiport activity in a pH-sensitive mutant . J. Biol. Chem. 255 : 3824 - 3825 .",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "p_23",
      "doi-asserted-by": "crossref",
      "first-page": "290",
      "DOI": "10.1007/BF00411252",
      "article-title": "Changes in turgor pressure in response to increases in external NaCI concentration in the gas-vacuolate cyanobacterium Microcystis sp",
      "volume": "143",
      "author": "Reed R. H.",
      "year": "1985",
      "unstructured": "Reed , R. H. , and A. E. Walsby . 1985 . Changes in turgor pressure in response to increases in external NaCI concentration in the gas-vacuolate cyanobacterium Microcystis sp . Arch. Microbiol. 143 : 290 - 296 .",
      "journal-title": "Arch. Microbiol."
    },
    {
      "key": "p_24",
      "doi-asserted-by": "crossref",
      "first-page": "283",
      "DOI": "10.1085/jgp.72.3.283",
      "article-title": "Cation transport in Escherichia coli. IX. Regulation of K+ transport",
      "volume": "72",
      "author": "Rhoads D. B.",
      "year": "1978",
      "unstructured": "Rhoads , D. B. , and W. Epstein . 1978 . Cation transport in Escherichia coli. IX. Regulation of K+ transport . J. Gen. Physiol. 72 : 283 - 295 .",
      "journal-title": "J. Gen. Physiol."
    },
    {
      "key": "p_25",
      "doi-asserted-by": "crossref",
      "first-page": "325",
      "DOI": "10.1085/jgp.67.3.325",
      "article-title": "Cation transport in Escherichia coli. VIII. Potassium transport mutants",
      "volume": "67",
      "author": "Rhoads D. B.",
      "year": "1976",
      "unstructured": "Rhoads , D. B. , F. B. Waters , and W. Epstein . 1976 . Cation transport in Escherichia coli. VIII. Potassium transport mutants . J. Gen. Physiol. 67 : 325 - 341 .",
      "journal-title": "J. Gen. Physiol."
    },
    {
      "key": "p_26",
      "first-page": "547",
      "article-title": "Measurement of membrane potential in bacteria",
      "volume": "55",
      "author": "Rottenberg H.",
      "year": "1979",
      "unstructured": "Rottenberg , H. 1979 . Measurement of membrane potential in bacteria . Methods Enzymol. 55 : 547 - 569 .",
      "journal-title": "Methods Enzymol."
    },
    {
      "key": "p_27",
      "unstructured": "Silver S. 1978. Transport of cations and anions p. 221-234. In B. P. Rosen (ed.) Bacterial transport. Marcel Dekker Inc. New York."
    },
    {
      "key": "p_28",
      "doi-asserted-by": "crossref",
      "unstructured": "Steinbach H. R. 1962. Comparative biochemistry of the alkali metals vol. 4 p. 677-720. In M. Florkin and H. Mason (ed.) Comparative biochemistry. Academic Press Inc. New York.",
      "DOI": "10.1016/B978-0-12-395545-6.50022-5"
    },
    {
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      "doi-asserted-by": "crossref",
      "first-page": "79",
      "DOI": "10.1111/j.1574-6968.1986.tb01846.x",
      "article-title": "Genetics of osmoregulation in Escherichia coli: uptake and biosynthesis of organic osmolytes",
      "volume": "37",
      "author": "Strom A. R.",
      "year": "1986",
      "unstructured": "Strom , A. R. , P. Falkenberg , and B. Landfald . 1986 . Genetics of osmoregulation in Escherichia coli: uptake and biosynthesis of organic osmolytes . FEMS Microbiol. Rev. 37 : 79 - 86 .",
      "journal-title": "FEMS Microbiol. Rev."
    },
    {
      "key": "p_30",
      "doi-asserted-by": "crossref",
      "first-page": "551",
      "DOI": "10.1016/0006-291X(73)90875-9",
      "article-title": "Galactoside transport dissociated from proton movements in mutants of Escherichia coli",
      "volume": "50",
      "author": "West I. C.",
      "year": "1973",
      "unstructured": "West , I. C. , and T. H. Wilson . 1973 . Galactoside transport dissociated from proton movements in mutants of Escherichia coli . Biochem. Biophys. Res. Commun. 50 : 551 - 558 .",
      "journal-title": "Biochem. Biophys. Res. Commun."
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