Synthesis of linear multimers of OriC and pBR322 derivatives in Escherichia coli K-12: role of recombination and replication functions
10.1128/jb.169.7.3131-3137.1987
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Inactivation of RecBCD nuclease (exonuclease V) and SbcB nuclease (exonuclease I) in Escherichia coli K-12 diverts most of plasmid replication activity from circular monomer production to the synthesis of linear multimers. Linear multimer synthesis has been demonstrated in plasmids of diverse origins and copy numbers, including E. coli minichromosomes. The effect of dnaA, dnaB, recF, and recJ mutations on the rate of linear multimer synthesis in sbcB cells after gam inactivation of RecBCD nuclease was investigated. Results are consistent with the hypothesis that homologous recombination, but not activities at the plasmid origin of replication, is involved in initiation of linear multimer synthesis.

Bibliography

Silberstein, Z., & Cohen, A. (1987). Synthesis of linear multimers of OriC and pBR322 derivatives in Escherichia coli K-12: role of recombination and replication functions. Journal of Bacteriology, 169(7), 3131–3137.

Authors 2
  1. Z Silberstein (first)
  2. A Cohen (additional)
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Dates
Type When
Created 8 years, 9 months ago (Nov. 2, 2016, 7:02 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:39 p.m.)
Indexed 1 year, 2 months ago (June 21, 2024, 5:32 p.m.)
Issued 38 years, 2 months ago (July 1, 1987)
Published 38 years, 2 months ago (July 1, 1987)
Published Print 38 years, 2 months ago (July 1, 1987)
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@article{Silberstein_1987, title={Synthesis of linear multimers of OriC and pBR322 derivatives in Escherichia coli K-12: role of recombination and replication functions}, volume={169}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.169.7.3131-3137.1987}, DOI={10.1128/jb.169.7.3131-3137.1987}, number={7}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Silberstein, Z and Cohen, A}, year={1987}, month=jul, pages={3131–3137} }