Gene fusion is a possible mechanism underlying the evolution of STA1
10.1128/jb.169.5.2142-2149.1987
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

DNA from the STA1 (extracellular glucoamylase) gene of Saccharomyces diastaticus was used as a probe to enable the cloning by colony hybridization of three DNA fragments from Saccharomyces cerevisiae; these were designated S1, S2, and SGA (intracellular, sporulation-specific glucoamylase gene). To examine the evolutionary relationship among these sequences at the nucleotide level, we sequenced S2, S1, SGA and compared them with STA1. These data and RNA blot analysis revealed that the following regions of STA1 were highly conserved in S2, S1, and SGA: upstream regulatory sequences responsible for transcription, a signal sequence for protein secretion, a threonine- and serine-rich domain, and a catalytic domain for glucoamylase activity. These results suggest that an ancestral STA gene was generated relatively recently in an evolutionary time scale by the sequential fusions of S2, S1, and SGA, with S1 functioning as a connector for S2 and SGA. We describe a model for the involvement of short nucleotide sequences flanking the junctions in the gene fusions.

Bibliography

Yamashita, I., Nakamura, M., & Fukui, S. (1987). Gene fusion is a possible mechanism underlying the evolution of STA1. Journal of Bacteriology, 169(5), 2142–2149.

Authors 3
  1. I Yamashita (first)
  2. M Nakamura (additional)
  3. S Fukui (additional)
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Dates
Type When
Created 8 years, 9 months ago (Nov. 2, 2016, 7:42 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:37 p.m.)
Indexed 1 month, 2 weeks ago (July 14, 2025, 11:22 p.m.)
Issued 38 years, 4 months ago (May 1, 1987)
Published 38 years, 4 months ago (May 1, 1987)
Published Print 38 years, 4 months ago (May 1, 1987)
Funders 0

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@article{Yamashita_1987, title={Gene fusion is a possible mechanism underlying the evolution of STA1}, volume={169}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.169.5.2142-2149.1987}, DOI={10.1128/jb.169.5.2142-2149.1987}, number={5}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Yamashita, I and Nakamura, M and Fukui, S}, year={1987}, month=may, pages={2142–2149} }