Reconstitution of signaling in bacterial chemotaxis
10.1128/jb.169.5.1878-1885.1987
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Strains missing several genes required for chemotaxis toward amino acids, peptides, and certain sugars were tethered and their rotational behavior was analyzed. Null strains (called gutted) were deleted for genes that code for the transducers Tsr, Tar, Tap, and Trg and for the cytoplasmic proteins CheA, CheW, CheR, CheB, CheY, and CheZ. Motor switch components were wild type, flaAII(cheC), or flaBII(cheV). Gutted cells with wild-type motors spun exclusively counterclockwise, while those with mutant motors changed their directions of rotation. CheY reduced the bias (the fraction of time that cells spun counterclockwise) in either case. CheZ offset the effect of CheY to an extent that varied with switch allele but did not change the bias when tested alone. Transducers also increased the bias in the presence of CheY but not when tested alone. However, cells containing transducers and CheY failed to respond to attractants or repellents normally detected in the periplasm. This sensitivity was restored by addition of CheA and CheW. Thus, CheY both enhances clockwise rotation and couples the transducers to the flagella. CheZ acts, at the level of the motor, as a CheY antagonist. CheA or CheW or both are required to complete the signal pathway. A model is presented that explains these results and is consistent with other data found in the literature.

Bibliography

Wolfe, A. J., Conley, M. P., Kramer, T. J., & Berg, H. C. (1987). Reconstitution of signaling in bacterial chemotaxis. Journal of Bacteriology, 169(5), 1878–1885.

Authors 4
  1. A J Wolfe (first)
  2. M P Conley (additional)
  3. T J Kramer (additional)
  4. H C Berg (additional)
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Dates
Type When
Created 8 years, 9 months ago (Nov. 2, 2016, 7:42 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:37 p.m.)
Indexed 3 weeks, 2 days ago (Aug. 6, 2025, 9:55 a.m.)
Issued 38 years, 3 months ago (May 1, 1987)
Published 38 years, 3 months ago (May 1, 1987)
Published Print 38 years, 3 months ago (May 1, 1987)
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@article{Wolfe_1987, title={Reconstitution of signaling in bacterial chemotaxis}, volume={169}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.169.5.1878-1885.1987}, DOI={10.1128/jb.169.5.1878-1885.1987}, number={5}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Wolfe, A J and Conley, M P and Kramer, T J and Berg, H C}, year={1987}, month=may, pages={1878–1885} }