DOI: 10.1128/jb.169.2.572-578.1987. Molecular characterization and nucleic acid sequence of an avirulence gene from race 6 of Pseudomonas syringae pv. glycinea

Molecular characterization and nucleic acid sequence of an avirulence gene from race 6 of Pseudomonas syringae pv. glycinea

10.1128/jb.169.2.572-578.1987

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

A gene was previously cloned from Pseudomonas syringae pv. glycinea race 6, designated avirulence gene A (avrA), that controls the expression of virulence by the pathogen on specific cultivars of soybean. A 3.2-kilobase (kb) AccI subclone from the cosmid clone pPg6L3 was shown to be active when cloned into the broad-host-range vector pRK404. Transposon Tn5 mutagenesis and deletion analysis delineated a span of approximately 2.5 kb of DNA that was necessary for gene activity. The nucleotide sequence of a 3.409-kb segment of DNA which contained the avrA gene has been determined. An open reading frame of 2.721 kb of DNA, which correlates with the region of DNA defined by transposon mutagenesis and deletion analysis, was identified. The open reading frame would encode a protein of 100.866 kilodaltons, which is in good agreement with the 100-kilodalton protein expressed by Escherichia coli maxicells.

Bibliography

Napoli, C., & Staskawicz, B. (1987). Molecular characterization and nucleic acid sequence of an avirulence gene from race 6 of Pseudomonas syringae pv. glycinea. Journal of Bacteriology, 169(2), 572â578.

Authors 2

C Napoli (first)
B Staskawicz (additional)

References 19 Referenced 52

Ditta , G. , S. Stanfield , D. Corbin , and D. Helinski . 1980 . Broad host range DNA cloning system for gram negative bacteria: construction of a gene bank of Rhizobium meliloti . Proc. Natl. Acad. Sci. USA 77 : 7347 - 7351 . (10.1073/pnas.77.12.7347) / Proc. Natl. Acad. Sci. USA / Broad host range DNA cloning system for gram negative bacteria: construction of a gene bank of Rhizobium meliloti by Ditta G. (1980)
Ellingboe A. 1982. Genetical aspects of active defence p. 179-192. In R. K. S. Wood (ed.) Active defence mechanisms of plants. Plenum Publishing Corp. London. (10.1007/978-1-4615-8309-7_10)
Flor , A. H. 1947 . Inheritance of reaction to rust in flax . J. Agric. Res. 74 : 241 - 262 . / J. Agric. Res. / Inheritance of reaction to rust in flax by Flor A. H. (1947)
Flor , A. H. 1947 . Host-parasite interactions in flax rust - its genetics and other implications . Phytopathology 45 : 680 - 685 . / Phytopathology / Host-parasite interactions in flax rust - its genetics and other implications by Flor A. H. (1947)
Gold , L. , D. Pribnow , T. Schneider , S. Shinedling , B. S. Singer , and G. Stormo . 1981 . Translational initiation in prokaryotes. Annu . Rev. Microbiol. 35 : 365 - 403 . (10.1146/annurev.mi.35.100181.002053) / Rev. Microbiol. / Translational initiation in prokaryotes. Annu by Gold L. (1981)
Hopp , T. P. , and K. R. Woods . 1981 . Prediction of protein antigenic determinants from amino acid sequences . Proc. Natl. Acad. Sci. USA 78 : 3824 - 3828 . (10.1073/pnas.78.6.3824) / Proc. Natl. Acad. Sci. USA / Prediction of protein antigenic determinants from amino acid sequences by Hopp T. P. (1981)
Keen N. and M. Holiday. 1982. Recognition of bacterial pathogens by plants p. 179-217. In M. Mount and G. H. Lacy (ed.) Phytopathogenic procaryotes vol. II. Academic Press Inc. New York. (10.1016/B978-0-12-509002-5.50018-5)
King , E. O. , M. K. Ward , and D. E. Raney . 1954 . Two simple media for the demonstration of pyocyanin and fluorescein . J. Lab. Clin. Med. 44 : 301 - 307 . / J. Lab. Clin. Med. / Two simple media for the demonstration of pyocyanin and fluorescein by King E. O. (1954)
Kyte , J. , and R. F. Doolittle . 1982 . A simple method for displaying the hydropathic character of a protein . J. Mol. Biol. 57 : 105 - 132 . (10.1016/0022-2836(82)90515-0) / J. Mol. Biol. / A simple method for displaying the hydropathic character of a protein by Kyte J. (1982)
Laemmli , U. K. 1970 . Cleavage of structural proteins -during the assembly of the head of bacteriophage T4 . Nature (London) 227 : 680 - 685 . (10.1038/227680a0) / Nature (London) / Cleavage of structural proteins -during the assembly of the head of bacteriophage T4 by Laemmli U. K. (1970)
Maniatis T. E. Fritsch and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
Miler J. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
Rosenberg , M. , and D. Court . 1979 . Regulatory sequences involved in the promotion and termination of RNA transcription. Annu . Rev. Genet. 13 : 319 - 353 . (10.1146/annurev.ge.13.120179.001535) / Rev. Genet. / Regulatory sequences involved in the promotion and termination of RNA transcription. Annu by Rosenberg M. (1979)
Ruvkun , G. , and F. Ausubel . 1981 . A general method for site-directed mutagenesis in prokaryotes . Nature (London) 489 : 75 - 78 . / Nature (London) / A general method for site-directed mutagenesis in prokaryotes by Ruvkun G. (1981)
Sancar , A. , A. M. Hack , and W. D. Rupp . 1979 . Simple method for the identification of plasmid-coded proteins . J. Bacteriol. 137 : 692 - 693 . (10.1128/jb.137.1.692-693.1979) / J. Bacteriol. / Simple method for the identification of plasmid-coded proteins by Sancar A. (1979)
Sanger , F. , S. Nicklen , and A. R. Coulson . 1977 . DNA sequencing with chain-terminating inhibitors . Proc. Natl. Acad. Sci. USA 74 : 5463 - 5467 . (10.1073/pnas.74.12.5463) / Proc. Natl. Acad. Sci. USA / DNA sequencing with chain-terminating inhibitors by Sanger F. (1977)
Shine , J. , and L. Dalgarno . 1974 . The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementary to nonsense triplets and ribosome binding sites . Proc. Natl. Acad. Sci. USA 71 : 1342 - 1346 . (10.1073/pnas.71.4.1342) / Proc. Natl. Acad. Sci. USA / The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementary to nonsense triplets and ribosome binding sites by Shine J. (1974)
Staskawicz , B. J. , D. Dahlbeck , and N. Keen . 1984 . Cloned avirulence gene of Pseudomonas syringae pv. glycinea determines race-specific incompatibility on Glycine max . Proc. Natl. Acad. Sci. USA 81 : 6024 - 6028 . (10.1073/pnas.81.19.6024) / Proc. Natl. Acad. Sci. USA / Cloned avirulence gene of Pseudomonas syringae pv. glycinea determines race-specific incompatibility on Glycine max by Staskawicz B. J. (1984)
von Heijne , G. 1985 . Signal sequences: the limits of variation . J. Mol. Biol. 184 : 99 - 105 . (10.1016/0022-2836(85)90046-4) / J. Mol. Biol. / Signal sequences: the limits of variation by von Heijne G. (1985)

Dates

Type	When
Created	8 years, 9 months ago (Nov. 2, 2016, 6:42 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:37 p.m.)
Indexed	2 months, 4 weeks ago (June 3, 2025, 8:09 p.m.)
Issued	38 years, 7 months ago (Feb. 1, 1987)
Published	38 years, 7 months ago (Feb. 1, 1987)
Published Print	38 years, 7 months ago (Feb. 1, 1987)

Funders 0

None

BibTeX

@article{Napoli_1987, title={Molecular characterization and nucleic acid sequence of an avirulence gene from race 6 of Pseudomonas syringae pv. glycinea}, volume={169}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.169.2.572-578.1987}, DOI={10.1128/jb.169.2.572-578.1987}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Napoli, C and Staskawicz, B}, year={1987}, month=feb, pages={572–578} }

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