DOI: 10.1128/jb.169.1.93-96.1987. Cloning and analysis of transcription of the mei2 gene responsible for initiation of meiosis in the fission yeast Schizosaccharomyces pombe

Cloning and analysis of transcription of the mei2 gene responsible for initiation of meiosis in the fission yeast Schizosaccharomyces pombe

10.1128/jb.169.1.93-96.1987

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

We have isolated a hybrid plasmid, pDB(mei2)2, containing a 7.4-kilobases (kb) DNA fragment from a Schizosaccharomyces pombe genomic library which is able to complement the mei2 mutation of S. pombe. Integration of the cloned DNA sequence at the mei2 site on chromosome I demonstrated that it contained the mei2 gene. This gene was localized on a 4.7-kb HindIII-PvuII fragment in the subclone pFMV402. Transcriptional regulation was studied by Northern blot analysis in which polyadenylated RNA was prepared from a heterozygous (h+N/h-S) diploid strain cultured either in nitrogen-rich growth medium or in nitrogen-free sporulation medium. The size of the major mei2 mRNA, which always gave a broad band, was estimated to be 4.2 +/- 0.2 kb, and a few minor bands (e.g., 3.2 and 1.8 kb) appeared as well. These transcripts appeared more abundantly in sporulating cells than in growing cells. Neither the mating type genes (mat) nor the mei3 gene was essential for transcription of the mei2 gene, since ample mei2 mRNA was detected in sporulation-deficient cells transferred to sporulation medium, such as h+N/h+N and h-S/h-S homozygotes, as well as mei1 and mei3 mutants.

Bibliography

Shimoda, C., Uehira, M., Kishida, M., Fujioka, H., Iino, Y., Watanabe, Y., & Yamamoto, M. (1987). Cloning and analysis of transcription of the mei2 gene responsible for initiation of meiosis in the fission yeast Schizosaccharomyces pombe. Journal of Bacteriology, 169(1), 93â96.

Authors 7

C Shimoda (first)
M Uehira (additional)
M Kishida (additional)
H Fujioka (additional)
Y Iino (additional)
Y Watanabe (additional)
M Yamamoto (additional)

References 17 Referenced 45

Beach , D. H. , and P. Nurse . 1981 . High-frequency transformation of the fission yeast Schizosaccharomyces pombe . Nature (London) 290 : 140 - 142 . (10.1038/290140a0) / Nature (London) / High-frequency transformation of the fission yeast Schizosaccharomyces pombe by Beach D. H. (1981)
Beach , D. H. , M. Piper , and P. Nurse . 1982 . Construction of a Schizosaccharomyces pombe gene bank in a yeast bacterial shuttle vector and its use to isolate genes by complementation . Mol. Gen. Genet. 187 : 326 - 329 . (10.1007/BF00331138) / Mol. Gen. Genet. / Construction of a Schizosaccharomyces pombe gene bank in a yeast bacterial shuttle vector and its use to isolate genes by complementation by Beach D. H. (1982)
Beach , D. , L. Rodgers , and J. Gould . 1985 . RANJ+ controls the transition from mitotic division to meiosis in fission yeast . Curr. Genet. 10 : 297 - 311 . (10.1007/BF00365626) / Curr. Genet. / RANJ+ controls the transition from mitotic division to meiosis in fission yeast by Beach D. (1985)
Botstein , D. , S. C. Falco , S. E. Stewart , M. Brennan , S. Scherer , D. T. Stinchcomb , K. Struhl , and R. W. Davis . 1979 . Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments . Gene 8 : 17 - 24 . (10.1016/0378-1119(79)90004-0) / Gene / Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments by Botstein D. (1979)
Bresch , C. , G. M Uller , and R. Egel . 1968 . Genes involved in meiosis and sporulation of a yeast . Mol. Gen. Genet. 102 : 301 - 306 . (10.1007/BF00433721) / Mol. Gen. Genet. / Genes involved in meiosis and sporulation of a yeast by Bresch C. (1968)
Davis R. W. D. Botstein and J. R. Roth. 1980. A manual for genetic engineering: advanced bacterial genetics. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
Davis , R. W. , M. Thomas , J. Cameron , T. P. St. John , S. Scherer , and R. A. Padgett . 1980 . Rapid DNA isolation for enzymatic and hybridization analysis . Methods Enzymol. 65 : 404 - 411 . (10.1016/S0076-6879(80)65051-4) / Methods Enzymol. / Rapid DNA isolation for enzymatic and hybridization analysis by Davis R. W. (1980)
Egel , R. 1971 . Physiological aspects of conjugation in fission yeast . Planta 98 : 89 - 96 . (10.1007/BF00387025) / Planta / Physiological aspects of conjugation in fission yeast by Egel R. (1971)
Egel , R. , and M. Egel-Mitani . 1974 . Premeiotic DNA synthesis in fission yeast . Exp. Cell Res. 88 : 127 - 134 . (10.1016/0014-4827(74)90626-0) / Exp. Cell Res. / Premeiotic DNA synthesis in fission yeast by Egel R. (1974)
Gutz H. H. Heslot U. Leupold and N. Loprieno. 1974. Schizosaccharomyces pombe p. 395-446. In R. C. King (ed.) Handbook of genetics. Plenum Publishing Co. New York. (10.1007/978-1-4899-1710-2_25)
Iino , Y. , and M. Yamamoto . 1985 . Mutants of Schizosaccharomyces pombe which sporulate in the haploid state . Mol. Gen. Genet. 198 : 416 - 421 . (10.1007/BF00332932) / Mol. Gen. Genet. / Mutants of Schizosaccharomyces pombe which sporulate in the haploid state by Iino Y. (1985)
Iino , Y. , and M. Yamamoto . 1985 . Negative control for the initiation of meiosis in Schizosaccharomyces pombe . Proc. Natl. Acad. Sci. USA 82 : 2447 - 2451 . (10.1073/pnas.82.8.2447) / Proc. Natl. Acad. Sci. USA / Negative control for the initiation of meiosis in Schizosaccharomyces pombe by Iino Y. (1985)
Jensen , R. , G. F. Sprague , Jr. , and I. Herskowitz . 1983 . Regulation of yeast mating-type interconversion: feedback control of HO gene expression by the mating-type locus . Proc. Natl. Acad. Sci. USA 80 : 3035 - 3039 . (10.1073/pnas.80.10.3035) / Proc. Natl. Acad. Sci. USA / Regulation of yeast mating-type interconversion: feedback control of HO gene expression by the mating-type locus by Jensen R. (1983)
Nakanishi , N. , and M. Yamamoto . 1984 . Analysis of the structure and transcription of the aro 3 cluster gene in Schizosaccharomyces pombe . Mol. Gen. Genet. 195 : 164 - 169 . (10.1007/BF00332740) / Mol. Gen. Genet. / Analysis of the structure and transcription of the aro 3 cluster gene in Schizosaccharomyces pombe by Nakanishi N. (1984)
Nurse P. 1985 . Mutants of the fission yeast Schizosaccharomyces pombe which alter the shift between cell proliferation and sporulation . Mol. Gen. Genet. 198 : 497 - 502 . (10.1007/BF00332946) / Mol. Gen. Genet. / Mutants of the fission yeast Schizosaccharomyces pombe which alter the shift between cell proliferation and sporulation by Nurse P. (1985)
Shimoda , C. , A. Hirata , M. Kishida , T. Hashida , and K. Tanaka . 1985 . Characterization of meiosis-deficient mutants by electron microscopy and mapping of four essential genes in the fission yeast Schizosaccharomyces pombe . Mol. Gen. Genet. 200 : 252 - 257 . (10.1007/BF00425432) / Mol. Gen. Genet. / Characterization of meiosis-deficient mutants by electron microscopy and mapping of four essential genes in the fission yeast Schizosaccharomyces pombe by Shimoda C. (1985)
Shimoda , C. , and M. Uehira . 1985 . Cloning of the Schizosaccharomyces pombe mei 3 gene essential for initiation of meiosis . Mol. Gen. Genet. 201 : 353 - 356 . (10.1007/BF00425685) / Mol. Gen. Genet. / Cloning of the Schizosaccharomyces pombe mei 3 gene essential for initiation of meiosis by Shimoda C. (1985)

Dates

Type	When
Created	8 years, 9 months ago (Nov. 2, 2016, 4:28 p.m.)
Deposited	4 years ago (July 29, 2021, 1:34 p.m.)
Indexed	1 month ago (July 24, 2025, 6:58 a.m.)
Issued	38 years, 7 months ago (Jan. 1, 1987)
Published	38 years, 7 months ago (Jan. 1, 1987)
Published Print	38 years, 7 months ago (Jan. 1, 1987)

Funders 0

None

BibTeX

@article{Shimoda_1987, title={Cloning and analysis of transcription of the mei2 gene responsible for initiation of meiosis in the fission yeast Schizosaccharomyces pombe}, volume={169}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.169.1.93-96.1987}, DOI={10.1128/jb.169.1.93-96.1987}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Shimoda, C and Uehira, M and Kishida, M and Fujioka, H and Iino, Y and Watanabe, Y and Yamamoto, M}, year={1987}, month=jan, pages={93–96} }

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