Naturally occurring TOL plasmids in Pseudomonas strains carry either two homologous or two nonhomologous catechol 2,3-oxygenase genes
10.1128/jb.168.2.878-885.1986
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Structural genes for catechol 2,3-oxygenase (C23O) were cloned from the TOL plasmids pWW5, pWW14, pWW74, pWW84, and pWW88 isolated from Pseudomonas strains of diverse geographical origins. Each pKT230-based C23O+ recombinant plasmid carried a 2.05-kilobase XhoI insert which showed strong homology in Southern hybridizations with the xylE gene from the archetype TOL plasmid pWW0. Fragments were mapped for restriction endonuclease sites and were classified into two closely related groups on the basis of restriction maps. C23O structural genes were located on cloned fragments by a combination of subcloning and site-specific mutagenesis. All five TOL plasmids examined yielded clones whose maps differed from that of xylE of pWW0 by only a single XbaI site, but in addition plasmids pWW5, pWW74, and pWW88 carried a second, homologous C23O gene with seven further restriction site differences. The remaining plasmids, pWW14 and pWW84, carried a second nonhomologous C23O gene related to the second C23O gene (C23OII) of TOL plasmid pWW15 described previously (H. Keil, M. R. Lebens, and P. A. Williams, J. Bacteriol. 163:248-255, 1985). Thus, each naturally occurring TOL plasmid in this study appears to carry genes for two meta cleavage dioxygenases.

Bibliography

Chatfield, L. K., & Williams, P. A. (1986). Naturally occurring TOL plasmids in Pseudomonas strains carry either two homologous or two nonhomologous catechol 2,3-oxygenase genes. Journal of Bacteriology, 168(2), 878–885.

Authors 2
  1. L K Chatfield (first)
  2. P A Williams (additional)
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Dates
Type When
Created 8 years, 8 months ago (Dec. 15, 2016, 7:43 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:34 p.m.)
Indexed 1 year, 5 months ago (March 20, 2024, 5:58 a.m.)
Issued 38 years, 10 months ago (Nov. 1, 1986)
Published 38 years, 10 months ago (Nov. 1, 1986)
Published Print 38 years, 10 months ago (Nov. 1, 1986)
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@article{Chatfield_1986, title={Naturally occurring TOL plasmids in Pseudomonas strains carry either two homologous or two nonhomologous catechol 2,3-oxygenase genes}, volume={168}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.168.2.878-885.1986}, DOI={10.1128/jb.168.2.878-885.1986}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Chatfield, L K and Williams, P A}, year={1986}, month=nov, pages={878–885} }