Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The virulence loci play an essential role in tumor formation by Agrobacterium tumefaciens. This study focused on the virC locus, which affects the host range Agrobacterium species. virC mutants display an attenuated or avirulent phenotype on certain host plants, but remain fully virulent on other plant hosts. The nucleotide sequence revealed that the virC locus of pTiA6NC is an operon consisting of two open reading frames. These two open reading frames, designated virC1 and virC2, encode protein products of 25,713 and 22,710 daltons, respectively, which were visualized by polyacrylamide gel electrophoresis. Only two nucleotides separated the stop codon for virC1 from the start codon for virC2, indicating that these genes may be translationally coupled.

Bibliography

Yanofsky, M. F., & Nester, E. W. (1986). Molecular characterization of a host-range-determining locus from Agrobacterium tumefaciens. Journal of Bacteriology, 168(1), 244–250.

Authors 2
  1. M F Yanofsky (first)
  2. E W Nester (additional)
References 35 Referenced 49
  1. Barry , G. F. , S. G. Rogers , R. T. Fraley , and L. Brand . 1984 . Identification of a cloned cytokinin biosynthetic gene . Proc. Natl. Acad. Sci. USA 81 : 4776 - 4780 . (10.1073/pnas.81.15.4776) / Proc. Natl. Acad. Sci. USA / Identification of a cloned cytokinin biosynthetic gene by Barry G. F. (1984)
  2. Bevan , M. W. , and M. -D. Chilton . 1982 . Multiple transcripts of T-DNA detected in nopaline crown gall tumors. Annu . Rev. Genet. 16 : 357 - 384 . / Rev. Genet. / Multiple transcripts of T-DNA detected in nopaline crown gall tumors. Annu by Bevan M. W. (1982)
  3. Birnboim , H. C. , and J. Doly . 1979 . A rapid alkaline extraction procedure for screening recombinant plasmid DNA . Nucleic Acids Res. 7 : 1513 - 1523 . (10.1093/nar/7.6.1513) / Nucleic Acids Res. / A rapid alkaline extraction procedure for screening recombinant plasmid DNA by Birnboim H. C. (1979)
  4. Chilton , M. - D. , T. Currier , S. Farrand , A. Bendich , M. Gordon , and E. Nester . 1974 . Agrobacterium DNA and PS9 bacteriophage DNA not detected in crown gall tumors . Proc. Natl. Acad. Sci. USA 71 : 3672 - 3676 . (10.1073/pnas.71.9.3672) / Proc. Natl. Acad. Sci. USA / Agrobacterium DNA and PS9 bacteriophage DNA not detected in crown gall tumors by Chilton M. (1974)
  5. Close , T. J. , R. C. Tait , and C. I. Kado . 1985 . Regulation of Ti plasmid virulence genes by a chromosomal locus of Agrobacterium tumefaciens . J. Bacteriol. 164 : 774 - 781 . (10.1128/jb.164.2.774-781.1985) / J. Bacteriol. / Regulation of Ti plasmid virulence genes by a chromosomal locus of Agrobacterium tumefaciens by Close T. J. (1985)
  6. Das , A. , S. Stachel , P. Ebert , P. Allenza , A. Montoya , and E. Nester . 1986 . Promoters of Agrobacterium tumefaciens Tiplasmid virulence genes . Nucleic Acids Res. 14 : 1355 - 1364 . (10.1093/nar/14.3.1355) / Nucleic Acids Res. / Promoters of Agrobacterium tumefaciens Tiplasmid virulence genes by Das A. (1986)
  7. Das , A. , and C. Yanofsky . 1984 . A ribosome binding site sequence is necessary for efficient expression of the distal gene of a translationally coupled gene pair . Nucleic Acids Res. 12 : 4757 - 4768 . (10.1093/nar/12.11.4757) / Nucleic Acids Res. / A ribosome binding site sequence is necessary for efficient expression of the distal gene of a translationally coupled gene pair by Das A. (1984)
  8. Depicker A. M. Van Montagu and J. Schell. 1983. Plant cell transformation by Agrobacterium plasmids p. 143-176. In T. Kosuge C. P. Meridith and A. Hollaender (ed.) Genetic engineering of plants: an agricultural perspective. Plenum Publishing Corp. New York. (10.1007/978-1-4684-4544-2_12)
  9. Garfinkel , D. J. , and E. W. Nester . 1980 . Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism . J. Bacteriol. 144 : 732 - 743 . (10.1128/jb.144.2.732-743.1980) / J. Bacteriol. / Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism by Garfinkel D. J. (1980)
  10. Hille , J. , J. van Kan , and R. Schilperoort . 1984 . trans-acting virulence functions of the octopine Ti plasmid from Agrobacterium tumefaciens . J. Bacteriol. 158 : 754 - 756 . (10.1128/jb.158.2.754-756.1984) / J. Bacteriol. / trans-acting virulence functions of the octopine Ti plasmid from Agrobacterium tumefaciens by Hille J. (1984)
  11. Hooykaas , P. J. J. , M. Hofker , H. Den Dulk-Ras , and R. A. Schilperoort . 1984 . A comparison of virulence determinants in an octopine Ti-plasmid, a nopaline Ti-plasmid, and an Riplasmid by complementation analysis of Agrobacterium tumefaciens mutants . Plasmid 11 : 195 - 205 . (10.1016/0147-619X(84)90026-X) / Plasmid / A comparison of virulence determinants in an octopine Ti-plasmid, a nopaline Ti-plasmid, and an Riplasmid by complementation analysis of Agrobacterium tumefaciens mutants by Hooykaas P. J. J. (1984)
  12. Hooykaas , P. J. J. , and R. A. Schilperoort . 1984 . The molecular genetics of crown gall tumorigenesis . Adv. Genet. 22 : 210 - 283 . / Adv. Genet. / The molecular genetics of crown gall tumorigenesis by Hooykaas P. J. J. (1984)
  13. . Horsch , R. B. , H. J. Klee , S. Stachel , S. C. Winans , E. W. Nester , S. G. Rogers , and R. T. Fraley . 1986 . Analysis of Agrobacterium tumefaciens virulence mutants in leaf discs . Proc. Natl. Acad. Sci. USA 83 : 2571 - 2575 . (10.1073/pnas.83.8.2571) / Proc. Natl. Acad. Sci. USA / Analysis of Agrobacterium tumefaciens virulence mutants in leaf discs by Horsch R. B. (1986)
  14. Klee , H. J. , M. P. Gordon , and E. W. Nester . 1982 . Complementation analysis of Agrobacterium tumefaciens Ti plasmid mutations affecting oncogenicity . J. Bacteriol. 150 : 327 - 331 . (10.1128/jb.150.1.327-331.1982) / J. Bacteriol. / Complementation analysis of Agrobacterium tumefaciens Ti plasmid mutations affecting oncogenicity by Klee H. J. (1982)
  15. Klee , H. J. , F. F. White , V. N. Iyer , M. P. Gordon , and E. W. Nester . 1983 . Mutational analysis of the virulence region of an Agrobacterium tumefaciens Ti plasmid . J. Bacteriol. 153 : 878 - 883 . (10.1128/jb.153.2.878-883.1983) / J. Bacteriol. / Mutational analysis of the virulence region of an Agrobacterium tumefaciens Ti plasmid by Klee H. J. (1983)
  16. Knauf , V. C. , and E. W. Nester . 1982 a. Wide host range cloning vectors: a cosmid bank of an Agrobacterium Ti-plasmid . Plasmid 8 : 45 - 54 . (10.1016/0147-619X(82)90040-3) / Plasmid / Wide host range cloning vectors: a cosmid bank of an Agrobacterium Ti-plasmid by Knauf V. C. (1982)
  17. Koukolikova-Nicola , Z. , R. E. Shillito , B. Hohn , K. Wang , M. Van Montagu , and P. Zambryski . 1985 . Involvement of circular intermediates in the transfer of T-DNA from Agrobacterium tumefaciens to plant cells . Nature (London) 313 : 191 - 196 . (10.1038/313191a0) / Nature (London) / Involvement of circular intermediates in the transfer of T-DNA from Agrobacterium tumefaciens to plant cells by Koukolikova-Nicola Z. (1985)
  18. Laemmli , U. K. 1970 . Cleavage of structural proteins during the assembly of the head of bacteriophage T4 . Nature (London) 277 : 680 - 685 . (10.1038/227680a0) / Nature (London) / Cleavage of structural proteins during the assembly of the head of bacteriophage T4 by Laemmli U. K. (1970)
  19. Lipman , D. J. , and W. R. Pearson . 1985 . Rapid and sensitive protein similarity searches . Science 227 : 1435 - 1441 . (10.1126/science.2983426) / Science / Rapid and sensitive protein similarity searches by Lipman D. J. (1985)
  20. Miller J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
  21. Morrissey , J. H. 1981 . Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity . Anal. Biochem. 117 : 307 - 310 . (10.1016/0003-2697(81)90783-1) / Anal. Biochem. / Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity by Morrissey J. H. (1981)
  22. Nester , E. W. , M. P. Gordon , R. M. Amasino , and M. F. Yanofsky . 1984 . Crown gall: a molecular and physiological analysis. Annu . Rev. Plant Physiol. 35 : 387 - 413 . (10.1146/annurev.pp.35.060184.002131) / Rev. Plant Physiol. / Crown gall: a molecular and physiological analysis. Annu by Nester E. W. (1984)
  23. Rosenberg , M. , and D. Court . 1979 . Regulatory sequences involved in the promotion and termination of RNA transcription. Annu . Rev. Genet. 13 : 319 - 354 . (10.1146/annurev.ge.13.120179.001535) / Rev. Genet. / Regulatory sequences involved in the promotion and termination of RNA transcription. Annu by Rosenberg M. (1979)
  24. Sancar , A. , R. P. Warton , S. Seltzer , B. M. Kacinski , N. D. Clarke , and W. D. Rupp . 1981 . Identification of the uvrA gene product . J. Mol. Biol. 148 : 45 - 62 . (10.1016/0022-2836(81)90234-5) / J. Mol. Biol. / Identification of the uvrA gene product by Sancar A. (1981)
  25. Sanger , F. , S. Nicklen , and A. R. Coulson . 1977 . DNA sequencing with chain-terminating inhibitors . Proc. Natl. Acad. Sci. USA 74 : 5463 - 5467 . (10.1073/pnas.74.12.5463) / Proc. Natl. Acad. Sci. USA / DNA sequencing with chain-terminating inhibitors by Sanger F. (1977)
  26. Schr 6der, G. , S. Waffenschmidt , E. W. Weiler , and J. Schr 6der. 1983 . The T-region of Ti-plasmids codes for an enzyme synthesizing indole-3-acetic acid . EMBO J. 2 : 403 - 409 . / EMBO J. / The T-region of Ti-plasmids codes for an enzyme synthesizing indole-3-acetic acid by Schr G. (1983)
  27. Shine , J. , and L. Dalgarno . 1974 . The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites . Proc. Natl. Acad. Sci. USA 71 : 1342 - 1346 . (10.1073/pnas.71.4.1342) / Proc. Natl. Acad. Sci. USA / The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites by Shine J. (1974)
  28. Spindler , K. , D. Rosser , and A. Berk . 1984 . Analysis of adenovirus transforming proteins from early regions 1A and 1B with antisera to inducible fusion antigens produced in Escherichia coli . Virology 49 : 132 - 141 . (10.1128/jvi.49.1.132-141.1984) / Virology / Analysis of adenovirus transforming proteins from early regions 1A and 1B with antisera to inducible fusion antigens produced in Escherichia coli by Spindler K. (1984)
  29. Stachel , S. E. , E. W. Nester , and P. C. Zambryski . 1985 . A plant cell factor induces Agrobacterium tumefaciens vir gene expression . Proc. Natl. Acad. Sci. USA 83 : 379 - 383 . (10.1073/pnas.83.2.379) / Proc. Natl. Acad. Sci. USA / A plant cell factor induces Agrobacterium tumefaciens vir gene expression by Stachel S. E. (1985)
  30. Thomashow , L. , S. Reeves , and M. F. Thomashow . 1984 . Crown gall oncogenesis: evidence that a T-DNA gene from the Agrobacterium Ti-plasmid pTiA6 encodes an enzyme that catalyzes synthesis of indoleacetic acid . Proc. Natl. Acad. Sci. USA 81 : 5071 - 5075 . (10.1073/pnas.81.16.5071) / Proc. Natl. Acad. Sci. USA / Crown gall oncogenesis: evidence that a T-DNA gene from the Agrobacterium Ti-plasmid pTiA6 encodes an enzyme that catalyzes synthesis of indoleacetic acid by Thomashow L. (1984)
  31. Vieira , J. , and J. Messing . 1982 . The pUC plasmids, an M13mp 7-derived system for insertion mutagenesis and sequencing with synthetic universal primers . Gene 19 : 259 - 268 . (10.1016/0378-1119(82)90015-4) / Gene / The pUC plasmids, an M13mp 7-derived system for insertion mutagenesis and sequencing with synthetic universal primers by Vieira J. (1982)
  32. Wang , K. , L. Herrera-Estrella , M. Van Montagu , and P. Zambryski . 1984 . Right 25 bp terminus sequence of the nopaline T-DNA is essential for and determines direction of DNA transfer from Agrobacterium to the plant genome . Cell 38 : 455 - 462 . (10.1016/0092-8674(84)90500-2) / Cell / Right 25 bp terminus sequence of the nopaline T-DNA is essential for and determines direction of DNA transfer from Agrobacterium to the plant genome by Wang K. (1984)
  33. Wray , W. , T. Boulikas , V. P. Wray , and R. Hancock . 1981 . Silver staining of proteins in polyacrylamide gels . Anal. Biochem. 118 : 197 - 203 . (10.1016/0003-2697(81)90179-2) / Anal. Biochem. / Silver staining of proteins in polyacrylamide gels by Wray W. (1981)
  34. Yanisch-Perron , C. , J. Vieira , and J. Messing . 1985 . Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl 8 and pUC19 vectors . Gene 33 : 103 - 119 . (10.1016/0378-1119(85)90120-9) / Gene / Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl 8 and pUC19 vectors by Yanisch-Perron C. (1985)
  35. Yanofsky , M. , B. Lowe , A. Montoya , R. Rubin , W. Krul , M. Gordon , and E. Nester . 1985 . Molecular and genetic analysis of factors controlling host range in Agrobacterium tumefaciens . Mol. Gen. Genet. 201 : 237 - 246 . (10.1007/BF00425665) / Mol. Gen. Genet. / Molecular and genetic analysis of factors controlling host range in Agrobacterium tumefaciens by Yanofsky M. (1985)
Dates
Type When
Created 8 years, 9 months ago (Nov. 2, 2016, 3:57 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:34 p.m.)
Indexed 1 year, 2 months ago (June 5, 2024, 5:33 a.m.)
Issued 38 years, 11 months ago (Oct. 1, 1986)
Published 38 years, 11 months ago (Oct. 1, 1986)
Published Print 38 years, 11 months ago (Oct. 1, 1986)
Funders 0

None

@article{Yanofsky_1986, title={Molecular characterization of a host-range-determining locus from Agrobacterium tumefaciens}, volume={168}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.168.1.244-250.1986}, DOI={10.1128/jb.168.1.244-250.1986}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Yanofsky, M F and Nester, E W}, year={1986}, month=oct, pages={244–250} }