Interaction of FtsA and PBP3 proteins in the Escherichia coli septum
10.1128/jb.166.3.985-992.1986
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Mutations in the ftsA gene of Escherichia coli conferred a higher resistance to lysis induced by penicillin or by a combination of cefsulodin and furazlocillin. The ftsA2 allele codes for an FtsA protein which is inactive at 42 degrees C but is able to regain its activity once it is transferred back to 30 degrees C; ftsA2 filaments formed at 42 degrees C in the presence of penicillin divided once the penicillin was removed and the temperature was lowered to 30 degrees C. Potential septation sites in the filaments of wild-type cells treated in the same way remained inactive. The binding of a radioactively labeled derivative of ampicillin to penicillin-binding protein 3 (PBP3) was significantly decreased in strain D-3, containing the mutant allele ftsA3, when the binding assay was performed at the restrictive temperature. A molecular species able to cross-react with an anti-PBP3 serum was nevertheless found to be present in the envelope of D-3 cells. These observations suggested that the FtsA protein, a protein with a structural and regulatory role in septation, and PBP3, a protein enzymatically active in the synthesis of murein for septation, interact with each other.

Bibliography

Tormo, A., Ayala, J. A., de Pedro, M. A., Aldea, M., & Vicente, M. (1986). Interaction of FtsA and PBP3 proteins in the Escherichia coli septum. Journal of Bacteriology, 166(3), 985–992.

Authors 5
  1. A Tormo (first)
  2. J A Ayala (additional)
  3. M A de Pedro (additional)
  4. M Aldea (additional)
  5. M Vicente (additional)
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Dates
Type When
Created 8 years, 9 months ago (Nov. 2, 2016, 5:37 p.m.)
Deposited 4 years ago (July 29, 2021, 1:32 p.m.)
Indexed 1 year, 7 months ago (Jan. 26, 2024, 12:22 a.m.)
Issued 39 years, 2 months ago (June 1, 1986)
Published 39 years, 2 months ago (June 1, 1986)
Published Print 39 years, 2 months ago (June 1, 1986)
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@article{Tormo_1986, title={Interaction of FtsA and PBP3 proteins in the Escherichia coli septum}, volume={166}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.166.3.985-992.1986}, DOI={10.1128/jb.166.3.985-992.1986}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Tormo, A and Ayala, J A and de Pedro, M A and Aldea, M and Vicente, M}, year={1986}, month=jun, pages={985–992} }