Treponema phagedenis has at least two proteins residing together on its periplasmic flagella
10.1128/jb.166.1.105-112.1986
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Treponema phagedenis is an anaerobic, motile spirochete with several periplasmic flagella (PFs) at each cell end. This study provides the first genetic evidence that multiple protein species are associated with the PFs. In addition, these proteins were found to reside together on a given PF. Nonmotile mutants which lacked the PFs were isolated, and spontaneous revertants to motility regained the PFs. These results suggest that the PFs are involved in the motility of T. phagedenis. Isolated PFs had two major protein bands with molecular weights of 33,000 and 39,800, as revealed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Western blots with monoclonal and polyclonal antibodies indicated that both proteins were absent in the PF mutants but present in the revertants. Immunoelectron microscopy revealed that the 39,800-molecular-weight protein was distributed along the entire PF. Immunoprecipitation analysis suggested that the 39,800- and 33,000-molecular-weight proteins were closely associated in situ.

Bibliography

Limberger, R. J., & Charon, N. W. (1986). Treponema phagedenis has at least two proteins residing together on its periplasmic flagella. Journal of Bacteriology, 166(1), 105–112.

Authors 2
  1. R J Limberger (first)
  2. N W Charon (additional)
References 43 Referenced 44
  1. Barbour , A. G. , S. L. Tessier , and S. F. Hayes . 1984 . Variation in a major surface protein of Lyme disease spirochetes . Infect. Immun. 45 : 94 - 100 . (10.1128/IAI.45.1.94-100.1984) / Infect. Immun. / Variation in a major surface protein of Lyme disease spirochetes by Barbour A. G. (1984)
  2. Barbour , A. G. , S. L. Tessier , and W. J. Todd . 1983 . Lyme disease spirochetes and ixodid tick spirochetes share a common surface antigenic determinant defined by a monoclonal antibody . Infect. Immun. 41 : 795 - 804 . (10.1128/IAI.41.2.795-804.1983) / Infect. Immun. / Lyme disease spirochetes and ixodid tick spirochetes share a common surface antigenic determinant defined by a monoclonal antibody by Barbour A. G. (1983)
  3. Berg , H. C. 1976 . How spirochetes may swim . J. Theor. Biol. 56 : 269 - 273 . (10.1016/S0022-5193(76)80074-4) / J. Theor. Biol. / How spirochetes may swim by Berg H. C. (1976)
  4. Berg , H. C. , D. B. Bromley , and N. W. Charon . 1978 . Leptospiral motility . Symp. Soc. Gen. Microbiol. 28 : 285 - 294 . / Symp. Soc. Gen. Microbiol. / Leptospiral motility by Berg H. C. (1978)
  5. Bharier , M. , and D. Allis . 1974 . Purification and characterization of axial filaments from Treponema phagedenis biotype reiterii (the Reiter treponeme) . J. Bacteriol. 120 : 1434 - 1442 . (10.1128/JB.120.3.1434-1442.1974) / J. Bacteriol. / Purification and characterization of axial filaments from Treponema phagedenis biotype reiterii (the Reiter treponeme) by Bharier M. (1974)
  6. Bharier , M. A. , and S. C. Rittenberg . 1971 . Chemistry of axial filaments of Treponema zuelzerae . J. Bacteriol. 105 : 422 - 429 . (10.1128/JB.105.1.422-429.1971) / J. Bacteriol. / Chemistry of axial filaments of Treponema zuelzerae by Bharier M. A. (1971)
  7. Bromley , D. B. , and N. W. Charon . 1979 . Axial filament involvement in the motility of Leptospira interrogans . J. Bacteriol. 137 : 1406 - 1412 . (10.1128/JB.137.3.1406-1412.1979) / J. Bacteriol. / Axial filament involvement in the motility of Leptospira interrogans by Bromley D. B. (1979)
  8. Cardwell , H. D. , and J. Schachter . 1982 . Antigenic analysis of the major outer membrane protein of Chlamydia spp . Infect. Immun. 35 : 1024 - 1031 . (10.1128/IAI.35.3.1024-1031.1982) / Infect. Immun. / Antigenic analysis of the major outer membrane protein of Chlamydia spp by Cardwell H. D. (1982)
  9. Canale-Parola , E. 1978 . Motility and chemotaxis of spirochetes. Annu . Rev. Microbiol. 32 : 69 - 99 . (10.1146/annurev.mi.32.100178.000441) / Rev. Microbiol. / Motility and chemotaxis of spirochetes. Annu by Canale-Parola E. (1978)
  10. Caterson , B. , J. E. Christner , and J. R. Baker . 1983 . Identification of a monoclonal antibody that specifically recognizes corneal and skeletal keratan sulfate . J. Biol. Chem. 258 : 8848 - 8854 . (10.1016/S0021-9258(18)32133-1) / J. Biol. Chem. / Identification of a monoclonal antibody that specifically recognizes corneal and skeletal keratan sulfate by Caterson B. (1983)
  11. De Pamphilis , M. L. , and J. Adler . 1971 . Purification of intact flagella from Escherichia coli and Bacillus subtilis . J. Bacteriol. 105 : 376 - 383 . (10.1128/JB.105.1.376-383.1971) / J. Bacteriol. / Purification of intact flagella from Escherichia coli and Bacillus subtilis by De Pamphilis M. L. (1971)
  12. Eipert , S. R. , and S. H. Black . 1979 . Characterization of the cytoplasmic fibrils of Treponema refringens (Nichols) . Arch. Microbiol. 120 : 205 - 214 . (10.1007/BF00423067) / Arch. Microbiol. / Characterization of the cytoplasmic fibrils of Treponema refringens (Nichols) by Eipert S. R. (1979)
  13. Emerson , S. U. , and M. I. Simon . 1971 . Variation in the primary structure of Bacillus subtilis flagellins . J. Bacteriol. 106 : 949 - 954 . (10.1128/jb.106.3.949-954.1971) / J. Bacteriol. / Variation in the primary structure of Bacillus subtilis flagellins by Emerson S. U. (1971)
  14. Frens , G. 1973 . Controlled nucleation for the regulation of the particle size in monodisperse gold solutions . Nature (London) Phys. Sci. 241 : 20 - 23 . (10.1038/physci241020a0) / Nature (London) Phys. Sci. / Controlled nucleation for the regulation of the particle size in monodisperse gold solutions by Frens G. (1973)
  15. Harboe , N. M. , and A. Ingild . 1983 . Immunization, isolation of immunoglobulins, and antibody titer determination . Scand. J. Immunol. 17(Suppl. 10 ): 345 - 351 . (10.1111/j.1365-3083.1983.tb04037.x) / Scand. J. Immunol. / Immunization, isolation of immunoglobulins, and antibody titer determination by Harboe N. M. (1983)
  16. Hardy , P. H. , Jr. , W. R. Fredericks , and E. E. Nell . 1975 . Isolation and antigenic characteristics of axial filaments from the Reiter treponeme . Infect. Immun. 11 : 380 - 386 . (10.1128/IAI.11.2.380-386.1975) / Infect. Immun. / Isolation and antigenic characteristics of axial filaments from the Reiter treponeme by Hardy P. H. (1975)
  17. Holt , S. C. 1978 . Anatomy and chemistry of spirochetes . Microbiol. Rev. 42 : 114 - 160 . (10.1128/MR.42.1.114-160.1978) / Microbiol. Rev. / Anatomy and chemistry of spirochetes by Holt S. C. (1978)
  18. Hovind Hougen , K. 1974 . The ultrastructure of cultivable treponemes. I. Treponema phagedenis, Treponema vincentii, and Treponema refrigens . Acta Pathol. Microbiol. Scand. Sect. B 82 : 329 - 344 . / Acta Pathol. Microbiol. Scand. Sect. B / The ultrastructure of cultivable treponemes. I. Treponema phagedenis, Treponema vincentii, and Treponema refrigens by Hovind Hougen K. (1974)
  19. Hovind Hougen , K. 1976 . Determination by means of electron microscopy of morphological criteria of value for classification of some spirochetes, in particular treponemes . Acta Pathol. Microbiol. Scand. Sect. B (Suppl.) 255 : 1 - 41 . / Acta Pathol. Microbiol. Scand. Sect. B (Suppl.) / Determination by means of electron microscopy of morphological criteria of value for classification of some spirochetes, in particular treponemes by Hovind Hougen K. (1976)
  20. Hovind Hougan , K. , and A. Birch-Andersen . 1971 . Electron microscopy of endoflagella and microtubules in Treponema Reiter . Acta Pathol. Microbiol. Scand. Sect. B. 79 : 37 - 50 . / Acta Pathol. Microbiol. Scand. Sect. B. / Electron microscopy of endoflagella and microtubules in Treponema Reiter by Hovind Hougan K. (1971)
  21. Johnson , R. C. , M. S. Wachter , and D. M. Ritzi . 1973 . Treponeme outer cell envelope: solubilization and reaggregation . Infect. Immun. 7 : 249 - 258 . (10.1128/IAI.7.2.249-258.1973) / Infect. Immun. / Treponeme outer cell envelope: solubilization and reaggregation by Johnson R. C. (1973)
  22. Joseph , R. , and E. Canale-Parola . 1972 . Axial fibrils of anaerobic spirochetes: ultrastructure and chemical characteristics . Arch. Mikrobiol. 181 : 146 - 168 . (10.1007/BF00412325) / Arch. Mikrobiol. / Axial fibrils of anaerobic spirochetes: ultrastructure and chemical characteristics by Joseph R. (1972)
  23. Kayser , A. 1979 . Geometry of spirochetes . FEMS Microbiol. Lett. 5 : 95 - 99 . (10.1111/j.1574-6968.1979.tb03255.x) / FEMS Microbiol. Lett. / Geometry of spirochetes by Kayser A. (1979)
  24. Koffler H. and R. W. Smith. 1972. The nature of flagellar antigens p. 22-66. In A. Nowotny (ed.) Cellular antigen. Springer-Verlag New York. (10.1007/978-1-4612-9843-4_4)
  25. Laemmli , U. K. 1970 . Cleavage of structural proteins during the assembly of the head of bacteriophage T4 . Nature (London) 227 : 680 - 685 . (10.1038/227680a0) / Nature (London) / Cleavage of structural proteins during the assembly of the head of bacteriophage T4 by Laemmli U. K. (1970)
  26. Lagenaur , C. , and N. Agabian . 1976 . Physical characterization of Caulobacter crescentus flagella . J. Bacteriol. 128 : 435 - 444 . (10.1128/JB.128.1.435-444.1976) / J. Bacteriol. / Physical characterization of Caulobacter crescentus flagella by Lagenaur C. (1976)
  27. Miao , R. , and A. H. Fieldsteel . 1978 . Genetics of Treponema: relationship between Treponema pallidum and five cultivable treponemes . J. Bacteriol. 133 : 101 - 107 . (10.1128/JB.133.1.101-107.1978) / J. Bacteriol. / Genetics of Treponema: relationship between Treponema pallidum and five cultivable treponemes by Miao R. (1978)
  28. Nauman , R. K. , S. C. Holt , and C. D. Cox . 1969 . Purification, ultrastructure, and composition of axial filaments from Leptospira . J. Bacteriol. 98 : 264 - 280 . (10.1128/JB.98.1.264-280.1969) / J. Bacteriol. / Purification, ultrastructure, and composition of axial filaments from Leptospira by Nauman R. K. (1969)
  29. Paster , B. J. , and E. Canale-Parola . 1980 . Involvement of periplasmic fibrils in motility of spirochetes . J. Bacteriol. 141 : 359 - 364 . (10.1128/jb.141.1.359-364.1980) / J. Bacteriol. / Involvement of periplasmic fibrils in motility of spirochetes by Paster B. J. (1980)
  30. Penn , C. W. , M. J. Bailey , and A. Cockayne . 1985 . The axial filament antigen of Treponema pallidum . Immunology 54 : 635 - 641 . / Immunology / The axial filament antigen of Treponema pallidum by Penn C. W. (1985)
  31. Ryter , A. , and J. Pillot . 1963 . Etude au microscope electronique de la structure externe et interne du treponeme Reiter. Ann. Inst. Pasteur (Paris) 104 : 496 - 501 . / Reiter. Ann. Inst. Pasteur (Paris) / Etude au microscope electronique de la structure externe et interne du treponeme by Ryter A. (1963)
  32. Sand Petersen , C. , N. S. Pedersen , and N. H. Axelsen . 1981 . A simple method for the isolation of flagella from Treponema Reiter . Acta Pathol. Microbiol. Scand. Sect. C 89 : 379 - 385 . / Acta Pathol. Microbiol. Scand. Sect. C / A simple method for the isolation of flagella from Treponema Reiter by Sand Petersen C. (1981)
  33. Shinoda , S. , T. Honda , Y. Takeda , and T. Miwatani . 1974 . Antigenic difference between polar monotrichous and peritrichous flagella of Vibro parahaemolyticus . J. Bacteriol. 120 : 923 - 928 . (10.1128/JB.120.2.923-928.1974) / J. Bacteriol. / Antigenic difference between polar monotrichous and peritrichous flagella of Vibro parahaemolyticus by Shinoda S. (1974)
  34. Silverman , M. , and M. I. Simon . 1977 . Bacterial flagella. Annu . Rev. Microbiol. 31 : 397 - 419 . (10.1146/annurev.mi.31.100177.002145) / Rev. Microbiol. / Bacterial flagella. Annu by Silverman M. (1977)
  35. Slot , J. W. , and H. J. Geuze . 1981 . Sizing of protein A-colloid gold probes for immunoelectron microscopy . J. Cell Biol. 90 : 533 - 536 . (10.1083/jcb.90.2.533) / J. Cell Biol. / Sizing of protein A-colloid gold probes for immunoelectron microscopy by Slot J. W. (1981)
  36. Smibert , R. M. 1984 . Genus III Treponema Schaudinn 1905 , ALp. 49 - 57 . In N. R. Krieg and J. G. Holt (ed.), Bergey's / ALp. / Genus III Treponema Schaudinn 1905 by Smibert R. M. (1984)
  37. manual of systematic bacteriology vol. 1. The Williams &
  38. Wilkins Co. Baltimore.
  39. Smith , R. W. , and H. Koffler . 1971 . Bacterial flagella . Adv. Microb. Physiol. 6 : 219 - 239 . (10.1016/S0065-2911(08)60070-3) / Adv. Microb. Physiol. / Bacterial flagella by Smith R. W. (1971)
  40. Sykes , J. A. , and J. N. Miller . 1973 . Ultrastructural studies of treponemes: location of axial filaments and some dimensions of Treponema pallidum (Nichols strain), Treponema denticola, and Treponema reiteri . Infect. Immun. 7 : 100 - 110 . (10.1128/IAI.7.1.100-110.1973) / Infect. Immun. / Ultrastructural studies of treponemes: location of axial filaments and some dimensions of Treponema pallidum (Nichols strain), Treponema denticola, and Treponema reiteri by Sykes J. A. (1973)
  41. Towbin , H. , T. Staehelin , and J. Gordon . 1979 . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications . Proc. Natl. Acad. Sci. USA 76 : 4350 - 4354 . (10.1073/pnas.76.9.4350) / Proc. Natl. Acad. Sci. USA / Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications by Towbin H. (1979)
  42. Weber , K. , and M. Osborn . 1969 . The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis . J. Biol. Chem. 244 : 4406 4412. (10.1016/S0021-9258(18)94333-4) / J. Biol. Chem. / The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis by Weber K. (1969)
  43. Weissborn , A. , H. M. Steinman , and L. Shapiro . 1982 . Characterization of the proteins of the Caulobacter crescentus flagellar filament. Peptide analysis and filament organization . J. Biol. Chem. 257 : 2066 - 2074 . (10.1016/S0021-9258(19)68147-0) / J. Biol. Chem. / Characterization of the proteins of the Caulobacter crescentus flagellar filament. Peptide analysis and filament organization by Weissborn A. (1982)
Dates
Type When
Created 8 years, 9 months ago (Nov. 2, 2016, 6:02 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:30 p.m.)
Indexed 1 year, 8 months ago (Dec. 21, 2023, 11:24 a.m.)
Issued 39 years, 4 months ago (April 1, 1986)
Published 39 years, 4 months ago (April 1, 1986)
Published Print 39 years, 4 months ago (April 1, 1986)
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@article{Limberger_1986, title={Treponema phagedenis has at least two proteins residing together on its periplasmic flagella}, volume={166}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.166.1.105-112.1986}, DOI={10.1128/jb.166.1.105-112.1986}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Limberger, R J and Charon, N W}, year={1986}, month=apr, pages={105–112} }