Identification and genetic analysis of sbcC mutations in commonly used recBC sbcB strains of Escherichia coli K-12
10.1128/jb.164.2.836-844.1985
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Evidence is presented to show that Escherichia coli JC7618, JC7621, and JC7623, previously regarded as having a recB recC sbcB genotype, carry an additional mutation in a new gene designated sbcC at minute 9 on the standard genetic map. In the absence of the sbcC mutation these strains are sensitive to mitomycin C and have a reduced efficiency of recombination. Cultures of recBC sbcB (sbcC+) strains grow slowly, contain many inviable cells, and rapidly accumulate fast-growing variants due to mutation of sbcC. sbcC has been identified on recombinant plasmids and tentatively located by Tn1000 mutagenesis to a 0.9-kilobase DNA section between proC and phoR.

Bibliography

Lloyd, R. G., & Buckman, C. (1985). Identification and genetic analysis of sbcC mutations in commonly used recBC sbcB strains of Escherichia coli K-12. Journal of Bacteriology, 164(2), 836–844.

Authors 2
  1. R G Lloyd (first)
  2. C Buckman (additional)
References 44 Referenced 155
  1. Bachmann , B. J. 1972 . Pedigrees of some mutant strains of Escherichia coli K-12 . Bacteriol. Rev. 36 : 525 - 557 . (10.1128/br.36.4.525-557.1972) / Bacteriol. Rev. / Pedigrees of some mutant strains of Escherichia coli K-12 by Bachmann B. J. (1972)
  2. Bachmann , B. J. 1983 . Linkage map of Escherichia coli K-12, edition 7 . Microbiol. Rev. 47 : 180 - 230 . (10.1128/mr.47.2.180-230.1983) / Microbiol. Rev. / Linkage map of Escherichia coli K-12, edition 7 by Bachmann B. J. (1983)
  3. Barbour , S. D. , H. Nagaishi , A. Templin , and A. J. Clark . 1970 . Biochemical and genetic studies of recombination proficiency in Escherichia coli II Rec+ revertants caused by indirect suppression of Rec- mutations . Proc. Natl. Acad. Sci. USA 67 : 128 - 135 . (10.1073/pnas.67.1.128) / Proc. Natl. Acad. Sci. USA / Biochemical and genetic studies of recombination proficiency in Escherichia coli II Rec+ revertants caused by indirect suppression of Rec- mutations by Barbour S. D. (1970)
  4. Brickman , E. , and J. Beckwith . 1975 . Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and 480 transducing phages . J. Mol. Biol. 96 : 307 - 316 . (10.1016/0022-2836(75)90350-2) / J. Mol. Biol. / Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and 480 transducing phages by Brickman E. (1975)
  5. Capaldo-Kimball , F. , and S. D. Barbour . 1971 . Involvement of recombination genes in growth and viability of Escherichia coli K-12 . J. Bacteriol. 106 : 204 - 212 . (10.1128/jb.106.1.204-212.1971) / J. Bacteriol. / Involvement of recombination genes in growth and viability of Escherichia coli K-12 by Capaldo-Kimball F. (1971)
  6. Clark , A. J. 1973 . Recombination deficient mutants of Escherichia coli and other bacteria. Annu . Rev. Genet. 7 : 67 - 86 . (10.1146/annurev.ge.07.120173.000435) / Rev. Genet. / Recombination deficient mutants of Escherichia coli and other bacteria. Annu by Clark A. J. (1973)
  7. Clark , A. J. 1980 . A view of the RecBC and RecF pathways of E. coli recombination . ICN-UCLA Symp. Mol. Cell. Biol. 29 : 891 - 899 . / ICN-UCLA Symp. Mol. Cell. Biol. / A view of the RecBC and RecF pathways of E. coli recombination by Clark A. J. (1980)
  8. Clark , A. J. , and A. D. Margulies . 1965 . Isolation and characterization of recombination-deficient mutants of Escherichia coli K12 . Proc. Natl. Acad. Sci. USA 53 : 451 - 459 . (10.1073/pnas.53.2.451) / Proc. Natl. Acad. Sci. USA / Isolation and characterization of recombination-deficient mutants of Escherichia coli K12 by Clark A. J. (1965)
  9. Cohen , A. , and A. Laban . 1983 . Plasmidic recombination in Escherichia coli K-12: the role of the recF gene function . Mol. Gen. Genet. 189 : 471 - 474 . (10.1007/BF00325911) / Mol. Gen. Genet. / Plasmidic recombination in Escherichia coli K-12: the role of the recF gene function by Cohen A. (1983)
  10. Gillen , J. R. , D. K. Willis , and A. J. Clark . 1981 . Genetic analysis of the RecE pathway of genetic recombination in Escherichia coli K-12 . J. Bacteriol. 145 : 521 - 532 . (10.1128/JB.145.1.521-532.1981) / J. Bacteriol. / Genetic analysis of the RecE pathway of genetic recombination in Escherichia coli K-12 by Gillen J. R. (1981)
  11. Goldmark , P. J. , and S. Linn . 1970 . An endonuclease activity from Escherichia coli absent from certain rec- strains . Proc. Natl. Acad. Sci. USA 67 : 434 441. (10.1073/pnas.67.1.434) / Proc. Natl. Acad. Sci. USA / An endonuclease activity from Escherichia coli absent from certain rec- strains by Goldmark P. J. (1970)
  12. Guyer , M. S. 1978 . The y8 sequence of F is an insertion sequence . J. Mol. Biol. 126 : 347 - 365 . (10.1016/0022-2836(78)90045-1) / J. Mol. Biol. / The y8 sequence of F is an insertion sequence by Guyer M. S. (1978)
  13. Hadley , R. G. , H. Ming , M. Timmons , K. Yun , and R. C. Donier . 1983 . A partial restriction map of the proA-purE region of the Escherichia coli K-12 chromosome . Gene 22 : 281 - 287 . (10.1016/0378-1119(83)90113-0) / Gene / A partial restriction map of the proA-purE region of the Escherichia coli K-12 chromosome by Hadley R. G. (1983)
  14. Haefher , K. 1968 . Spontaneous lethal sectoring, a further feature of Escherichia coli strains deficient in the function of rec and uvr genes . J. Bacteriol. 96 : 652 - 659 . (10.1128/jb.96.3.652-659.1968) / J. Bacteriol. / Spontaneous lethal sectoring, a further feature of Escherichia coli strains deficient in the function of rec and uvr genes by Haefher K. (1968)
  15. Horii , Z. I. , and A. J. Clark . 1973 . Genetic analysis of the recF pathway of genetic recombination in Escherichia coli K12: isolation and characterization of mutants . J. Mol. Biol. 80 : 327 - 344 . (10.1016/0022-2836(73)90176-9) / J. Mol. Biol. / Genetic analysis of the recF pathway of genetic recombination in Escherichia coli K12: isolation and characterization of mutants by Horii Z. I. (1973)
  16. Ish-Horowicz , D. , and J. F. Burke . 1981 . Rapid and efficient cosmid cloning . Nucleic Acids Res. 9 : 2989 - 2998 . (10.1093/nar/9.13.2989) / Nucleic Acids Res. / Rapid and efficient cosmid cloning by Ish-Horowicz D. (1981)
  17. Kleckner , N. 1981 . Transposable elements in prokaryotes. Annu . Rev. Genet. 15 : 341 - 404 . (10.1146/annurev.ge.15.120181.002013) / Rev. Genet. / Transposable elements in prokaryotes. Annu by Kleckner N. (1981)
  18. Kushner , S. R. , H. Nagaishi , and A. J. Clark . 1972 . Indirect suppression of recB and recC mutations by exonuclease I deficiency . Proc. Natl. Acad. Sci. USA 69 : 1366 - 1370 . (10.1073/pnas.69.6.1366) / Proc. Natl. Acad. Sci. USA / Indirect suppression of recB and recC mutations by exonuclease I deficiency by Kushner S. R. (1972)
  19. Kushner , S. R. , H. Nagaishi , A. Templin , and A. J. Clark . 1971 . Genetic recombination in Escherichia coli: the role of exonuclease I . Proc. Natl. Acad. Sci. USA 68 : 824 - 827 . (10.1073/pnas.68.4.824) / Proc. Natl. Acad. Sci. USA / Genetic recombination in Escherichia coli: the role of exonuclease I by Kushner S. R. (1971)
  20. Lloyd , R. G. , F. E. Benson , and C. E. Shurvinton . 1984 . Effect of ruv mutations on recombination and DNA repair in Escherichia coli K12 . Mol. Gen. Genet. 194 : 303 - 309 . (10.1007/BF00383532) / Mol. Gen. Genet. / Effect of ruv mutations on recombination and DNA repair in Escherichia coli K12 by Lloyd R. G. (1984)
  21. Lloyd , R. G. , and S. D. Barbour . 1974 . The genetic location of the sbcA gene of Escherichia coli . Mol. Gen. Genet. 134 : 157 - 171 . (10.1007/BF00268417) / Mol. Gen. Genet. / The genetic location of the sbcA gene of Escherichia coli by Lloyd R. G. (1974)
  22. Lloyd , R. G. , and S. Johnson . 1979 . Kinetics of recA function in conjugational recombinant formation . Mol. Gen. Genet. 169 : 219 - 228 . (10.1007/BF00271674) / Mol. Gen. Genet. / Kinetics of recA function in conjugational recombinant formation by Lloyd R. G. (1979)
  23. Lloyd , R. G. and B. Low . 1976 . Some genetic consequences of changes in the level of recA gene function in Escherichia coli K12 . Genetics 84 : 675 - 695 . (10.1093/genetics/84.4.675) / Genetics / Some genetic consequences of changes in the level of recA gene function in Escherichia coli K12 by Lloyd R. G. (1976)
  24. Lloyd , R. G. , B. Low , G. N. Godson , and E. A. Birge . 1974 . Isolation and characterization of an Escherichia coli K12 mutant with a temperature-sensitive RecA- phenotype . J. Bacteriol. 120 : 407 - 415 . (10.1128/JB.120.1.407-415.1974) / J. Bacteriol. / Isolation and characterization of an Escherichia coli K12 mutant with a temperature-sensitive RecA- phenotype by Lloyd R. G. (1974)
  25. Lloyd , R. G. , S. M. Picksley , and C. Prescott . 1983 . Inducible expression of a gene specific to the RecF pathway for recombination in Escherichia coli K12 . Mol. Gen. Genet. 167 : 162 - 167 . (10.1007/BF00330340) / Mol. Gen. Genet. / Inducible expression of a gene specific to the RecF pathway for recombination in Escherichia coli K12 by Lloyd R. G. (1983)
  26. Lloyd , R. G. , and A. Thomas . 1983 . On the nature of the RecBC and RecF pathways of conjugal recombination in Escherichia coli . Mol. Gen. Genet. 190 : 156 - 161 . (10.1007/BF00330339) / Mol. Gen. Genet. / On the nature of the RecBC and RecF pathways of conjugal recombination in Escherichia coli by Lloyd R. G. (1983)
  27. Lloyd , R. G. , and A. Thomas . 1984 . A molecular model for conjugational recombination in Escherichia coli K12 . Mol. Gen. Genet. 197 : 328 - 336 . (10.1007/BF00330981) / Mol. Gen. Genet. / A molecular model for conjugational recombination in Escherichia coli K12 by Lloyd R. G. (1984)
  28. Lovett , S. T. , and A. J. Clark . 1983 . Genetic analysis of regulation of the RecF pathway of recombination in Escherichia coli K-12 . J. Bacteriol. 153 : 1471 - 1478 . (10.1128/jb.153.3.1471-1478.1983) / J. Bacteriol. / Genetic analysis of regulation of the RecF pathway of recombination in Escherichia coli K-12 by Lovett S. T. (1983)
  29. Lovett , S. T. , and A. J. Clark . 1984 . Genetic analysis of the recJ gene of Escherichia coli K-12 . J. Bacteriol. 157 : 190 - 196 . (10.1128/jb.157.1.190-196.1984) / J. Bacteriol. / Genetic analysis of the recJ gene of Escherichia coli K-12 by Lovett S. T. (1984)
  30. Maniatis T. E. F. Fritsch and J. Sambrook. 1982. Molecular cloning a laboratory manual. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
  31. Masters , M. , B. J. Newman , and C. M. Henry . 1984 . Reduction of marker discrimination in transductional recombination . Mol. Gen. Genet. 196 : 81 - 84 . (10.1007/BF00334097) / Mol. Gen. Genet. / Reduction of marker discrimination in transductional recombination by Masters M. (1984)
  32. Nakayama , H. , K. Nakayama , N. Irono , Y. Nakayama , and P. C. Hanawalt . 1984 . Isolation and genetic characterization of a thymineless death-resistant mutant of Escherichia coli K12: identification of a new mutation (recQI) that blocks the RecF recombination pathway . Mol. Gen. Genet. 195 : 474 - 480 . (10.1007/BF00341449) / Mol. Gen. Genet. / Isolation and genetic characterization of a thymineless death-resistant mutant of Escherichia coli K12: identification of a new mutation (recQI) that blocks the RecF recombination pathway by Nakayama H. (1984)
  33. Picksley , S. M. , P. V. Attfield , and R. G. Lloyd . 1984 . Repair of DNA double-strand breaks in Escherichia coli K12 requires a functional recN product . Mol. Gen. Genet. 195 : 267 - 274 . (10.1007/BF00332758) / Mol. Gen. Genet. / Repair of DNA double-strand breaks in Escherichia coli K12 requires a functional recN product by Picksley S. M. (1984)
  34. Picksley , S. M. , R. G. Lloyd , and C. Buckman . 1984 . Genetic analysis and regulation of inducible recombination in Escherichia coli K-12 . Cold Spring Harbor Symp. Quant. Biol. 44 : 469 - 474 . (10.1101/SQB.1984.049.01.053) / Cold Spring Harbor Symp. Quant. Biol. / Genetic analysis and regulation of inducible recombination in Escherichia coli K-12 by Picksley S. M. (1984)
  35. Radding , C. 1982 . Homologous pairing and strand exchange in genetic recombination. Annu . Rev. Genet. 16 : 405 - 437 . (10.1146/annurev.ge.16.120182.002201) / Rev. Genet. / Homologous pairing and strand exchange in genetic recombination. Annu by Radding C. (1982)
  36. Shurvinton , C. E. , and R. G. Lloyd . 1982 . Damage to DNA induces expression of the ruv gene of Escherichia coli . Mol. Gen. Genet. 185 : 352 - 355 . (10.1007/BF00330811) / Mol. Gen. Genet. / Damage to DNA induces expression of the ruv gene of Escherichia coli by Shurvinton C. E. (1982)
  37. Shurvinton , C. E. , R. G. Lloyd , F. E. Benson , and P. V. Attfield . 1984 . Genetic analysis and molecular cloning of the Escherichia coli ruv gene . Mol. Gen. Genet. 194 : 322 - 329 . (10.1007/BF00383535) / Mol. Gen. Genet. / Genetic analysis and molecular cloning of the Escherichia coli ruv gene by Shurvinton C. E. (1984)
  38. Smith , G. R. , S. K. Amundsen , A. M. Chaudhury , K. C. Cheng , A. S. Ponticelli , C. M. Roberts , D. W. Shultz , and A. F. Taylor . 1984 . Roles of RecBC enzyme and Chi sites in homologous recombination . Cold Spring Harbor Symp. Quant. Biol. 44 : 485 - 495 . (10.1101/SQB.1984.049.01.055) / Cold Spring Harbor Symp. Quant. Biol. / Roles of RecBC enzyme and Chi sites in homologous recombination by Smith G. R. (1984)
  39. Stahl , F. W. , and M. M. Stahl . 1977 . Recombination pathway specificity of Chi . Genetics 86 : 715 - 725 . (10.1093/genetics/86.4.715) / Genetics / Recombination pathway specificity of Chi by Stahl F. W. (1977)
  40. Taylor , A. , and G. R. Smith . 1980 . Unwinding and rewinding of DNA by the RecBC enzyme . Cell 22 : 447457 . (10.1016/0092-8674(80)90355-4) / Cell / Unwinding and rewinding of DNA by the RecBC enzyme by Taylor A. (1980)
  41. Templin , A. , S. R. Kushner , and A. J. Clark . 1972 . Genetic analysis of mutations indirectly suppressing recB and recC mutations . Genetics 72 : 205 - 215 . (10.1093/genetics/72.2.205) / Genetics / Genetic analysis of mutations indirectly suppressing recB and recC mutations by Templin A. (1972)
  42. Tommassen J. , P. Hiemstra , P. Overduin , and B. Lugtenberg . 1984 . Cloning of phoM, a gene involved in regulation of the synthesis of phosphate limitation inducible proteins in Escherichia coli K12 . Mol. Gen. Genet. 195 : 190 - 194 . (10.1007/BF00332745) / Mol. Gen. Genet. / Cloning of phoM, a gene involved in regulation of the synthesis of phosphate limitation inducible proteins in Escherichia coli K12 by Tommassen J. (1984)
  43. Walker , G. C. 1984 . Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli . Microbiol. Rev. 48 : 60 - 93 . (10.1128/mr.48.1.60-93.1984) / Microbiol. Rev. / Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli by Walker G. C. (1984)
  44. Willets , N. S. , and D. W. Mount . 1969 . Genetic analysis of recombination-deficient mutants of Escherichia coli K-12 carrying rec mutations cotransducible with thyA . J. Bacteriol. 100 : 923 - 934 . (10.1128/JB.100.2.923-934.1969) / J. Bacteriol. / Genetic analysis of recombination-deficient mutants of Escherichia coli K-12 carrying rec mutations cotransducible with thyA by Willets N. S. (1969)
Dates
Type When
Created 5 years, 7 months ago (Jan. 3, 2020, 1:23 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:29 p.m.)
Indexed 3 weeks, 6 days ago (Aug. 5, 2025, 9:12 a.m.)
Issued 39 years, 10 months ago (Nov. 1, 1985)
Published 39 years, 10 months ago (Nov. 1, 1985)
Published Print 39 years, 10 months ago (Nov. 1, 1985)
Funders 0

None

@article{Lloyd_1985, title={Identification and genetic analysis of sbcC mutations in commonly used recBC sbcB strains of Escherichia coli K-12}, volume={164}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.164.2.836-844.1985}, DOI={10.1128/jb.164.2.836-844.1985}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Lloyd, R G and Buckman, C}, year={1985}, month=nov, pages={836–844} }