Isolation and expression of the Escherichia coli gene encoding malate dehydrogenase
10.1128/jb.163.3.1074-1079.1985
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

An oligodeoxynucleotide specific for a pentapeptide sequence corresponding to amino acid residues 32 through 36 of Escherichia coli malate dehydrogenase was chemically synthesized and used to identify the mdh gene on plasmid pLC32-38 from the Clarke-Carbon recombinant library. Cells transformed with this plasmid exhibited a 10-fold increase in malate dehydrogenase activity. A 1.2-kilobase PvuII fragment which hybridized with the oligodeoxynucleotide probe was subcloned, and the identity of the mdh structural gene was confirmed by partial nucleotide sequence analysis. The expression of the mdh gene, as measured by both Northern blotting and enzyme assays, was found to vary over a 20-fold range with different culture conditions.

Bibliography

Sutherland, P., & McAlister-Henn, L. (1985). Isolation and expression of the Escherichia coli gene encoding malate dehydrogenase. Journal of Bacteriology, 163(3), 1074–1079.

Authors 2
  1. P Sutherland (first)
  2. L McAlister-Henn (additional)
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Dates
Type When
Created 5 years, 8 months ago (Jan. 3, 2020, 1:22 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:29 p.m.)
Indexed 4 months, 3 weeks ago (April 9, 2025, 12:54 a.m.)
Issued 40 years ago (Sept. 1, 1985)
Published 40 years ago (Sept. 1, 1985)
Published Print 40 years ago (Sept. 1, 1985)
Funders 0

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@article{Sutherland_1985, title={Isolation and expression of the Escherichia coli gene encoding malate dehydrogenase}, volume={163}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.163.3.1074-1079.1985}, DOI={10.1128/jb.163.3.1074-1079.1985}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Sutherland, P and McAlister-Henn, L}, year={1985}, month=sep, pages={1074–1079} }