DOI: 10.1128/jb.162.1.413-419.1985. Manipulation of intracellular magnesium content in polymyxin B nonapeptide-sensitized Escherichia coli by ionophore A23187

Manipulation of intracellular magnesium content in polymyxin B nonapeptide-sensitized Escherichia coli by ionophore A23187

10.1128/jb.162.1.413-419.1985

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Escherichia coli B cells were sensitized to ionophore A23187 by polymyxin B nonapeptide, and the induced magnesium and potassium ion fluxes were studied. Combined ionophore treatment permeabilized the cytoplasmic membrane of E. coli in an ion-specific manner and allowed the manipulation of intracellular Mg2+ content from the outside. A23187-induced Mg2+ efflux or influx was dependent on the free Mg2+ concentration gradient between the outside and inside of the cytoplasmic membrane and on the pH gradient. Most of the intracellular Mg2+ was bound, whereas only 1 to 2 mM was free in solution in the cellular sap.

Bibliography

Alatossava, T., JÃ¼tte, H., Kuhn, A., & Kellenberger, E. (1985). Manipulation of intracellular magnesium content in polymyxin B nonapeptide-sensitized Escherichia coli by ionophore A23187. Journal of Bacteriology, 162(1), 413â419.

Authors 4

T Alatossava (first)
H Jütte (additional)
A Kuhn (additional)
E Kellenberger (additional)

References 22 Referenced 169

Alatossava , T. , M. Vaara , and W. Baschong . 1984 . Polymyxin B nonapeptide sensitizes E;scherichia coli to valinomycin and A23187 ionophores . FEMS Microbiol. Lett. 22 : 249 - 251 . (10.1111/j.1574-6968.1984.tb00736.x) / FEMS Microbiol. Lett. / Polymyxin B nonapeptide sensitizes E;scherichia coli to valinomycin and A23187 ionophores by Alatossava T. (1984)
Bakker , E. P. , and W. E. Mangerich . 1981 . Interconversion of components of the bacterial proton motive force by electrogenic potassium transport . J. Bacteriol. 147 : 820 - 826 . (10.1128/jb.147.3.820-826.1981) / J. Bacteriol. / Interconversion of components of the bacterial proton motive force by electrogenic potassium transport by Bakker E. P. (1981)
Duszynski , J. , and L. Wojtezak . 1977 . Effect of Mg 2' depletion of mitochondria on their permeability to K+: the mechanism by which ionophore A23187 increase K+ permeability . Biochem. Biophys. Res. Commun. 74 : 417 - 424 . (10.1016/0006-291X(77)90320-5) / Biochem. Biophys. Res. Commun. / Effect of Mg 2' depletion of mitochondria on their permeability to K+: the mechanism by which ionophore A23187 increase K+ permeability by Duszynski J. (1977)
Flatman , P. , and V. L. Leve . 1977 . Does ionophore A23187 mediate Na transport in the absence of divalent cations ? Nature (London) 270 : 444 445. (10.1038/270444a0) / Nature (London) / Does ionophore A23187 mediate Na transport in the absence of divalent cations by Flatman P. (1977)
Gunther , T. , and F. Dorn . 1969 . Ueber die intrazellulare Mg-Ionenaktivitfit von E. coli-Zellen . Z. Naturforsch. 24 : 713 - 717 . (10.1515/znb-1969-0608) / Z. Naturforsch. / Ueber die intrazellulare Mg-Ionenaktivitfit von E. coli-Zellen by Gunther T. (1969)
Harold , F. M. 1982 . Pumps and currents: a biological perspective . Curr. Top. Membr. Transp. 16 : 485 - 516 . (10.1016/S0070-2161(08)60716-9) / Curr. Top. Membr. Transp. / Pumps and currents: a biological perspective by Harold F. M. (1982)
Kauffman , R. F. , R. W. Taylor , and D. R. Pfeiffer . 1980 . Cation transport and specificity of ionomycin: comparison with ionophore A23187 in rat liver mitochondria . J. Biol. Chem. 255 : 2735 - 2739 . (10.1016/S0021-9258(19)85799-X) / J. Biol. Chem. / Cation transport and specificity of ionomycin: comparison with ionophore A23187 in rat liver mitochondria by Kauffman R. F. (1980)
Kroll , R. G. , and I. R. Booth . 1983 . The relationship between intracellular pH, the pH gradient and potassium transport in Escherichia coli . Biochem. J. 216 : 709 - 716 . (10.1042/bj2160709) / Biochem. J. / The relationship between intracellular pH, the pH gradient and potassium transport in Escherichia coli by Kroll R. G. (1983)
Kuhn , A. H. U. , H. Jutte , and E. Kellenberger . 1983 . Involvement of the bacterial groM gene product in bacteriophage T7 reproduction. II. A reduced level of ion concentrations causes the blockage of T7 maturation in K-12-M cells . J. Virol. 47 : 540 - 552 . (10.1128/JVI.47.3.540-552.1983) / J. Virol. / Involvement of the bacterial groM gene product in bacteriophage T7 reproduction. II. A reduced level of ion concentrations causes the blockage of T7 maturation in K-12-M cells by Kuhn A. H. U. (1983)
Lusk , J. E. , R. J. P. Williams , and E. P. Kennedy . 1968 . Magnesium and the growth of Escherichia coli . J. Biol. Chem. 243 : 2618 - 2624 . (10.1016/S0021-9258(18)93417-4) / J. Biol. Chem. / Magnesium and the growth of Escherichia coli by Lusk J. E. (1968)
Moncany , M. L. J. , and E. Kellenberger . 1981 . High magnesium content of Escherichia coli B . Experientia 37 : 846 - 847 . (10.1007/BF01985672) / Experientia / High magnesium content of Escherichia coli B by Moncany M. L. J. (1981)
Padan , E. , D. Zilberstein , and H. Rottenberg . 1976 . The proton electrochemical gradient in Escherichia coli cells . Eur. J. Biochem. 63 : 533 - 541 . (10.1111/j.1432-1033.1976.tb10257.x) / Eur. J. Biochem. / The proton electrochemical gradient in Escherichia coli cells by Padan E. (1976)
Padan , E. , D. Zilberstein , and S. Schuldiner . 1981 . pH homeostasis in bacteria . Biochim. Biophys. Acta 650 : 151 - 166 . (10.1016/0304-4157(81)90004-6) / Biochim. Biophys. Acta / pH homeostasis in bacteria by Padan E. (1981)
Pfeiffer , D. R. , and H. A. Lardy . 1976 . lonophore A23187: the effect of H+ concentration on complex formation with divalent and monovalent cations and the demonstration of K+ transport in mitochondria mediated by A23187 . Biochemistry 15 : 935 - 943 . (10.1021/bi00650a001) / Biochemistry / lonophore A23187: the effect of H+ concentration on complex formation with divalent and monovalent cations and the demonstration of K+ transport in mitochondria mediated by A23187 by Pfeiffer D. R. (1976)
Pfeiffer , D. R. , R. W. Taylor , and H. A. Lardy . 1978 . lonophore A23187: cation binding and transport properties . Ann. N. Y. Acad. Sci. 307 : 402423 . (10.1111/j.1749-6632.1978.tb41965.x) / Ann. N. Y. Acad. Sci. / lonophore A23187: cation binding and transport properties by Pfeiffer D. R. (1978)
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Silver S. 1978. Transport of cations and anions p. 221-324. In B. P. Rosen (ed.) Bacterial transport. Marcel Dekker Inc. New York.
Sokolove , P. M. 1979 . Conditions limiting the use of ionophore A23187 as a probe of divalent cation involvement in biological reactions: evidence from the slow fluorescence quenching of type A spinach chloroplasts . Biochim. Biophys. Acta 545 : 155 - 164 . (10.1016/0005-2728(79)90122-1) / Biochim. Biophys. Acta / Conditions limiting the use of ionophore A23187 as a probe of divalent cation involvement in biological reactions: evidence from the slow fluorescence quenching of type A spinach chloroplasts by Sokolove P. M. (1979)
Sussmuth , R. , and A. Widmann . 1979 . -y Bestrahlte Ribosomen von Micrococcus radiodurans in einem System der zellfreien Proteinsynthese . Z. Naturforsch. 34 : 565 - 569 . (10.1515/znc-1979-7-813) / Z. Naturforsch. / -y Bestrahlte Ribosomen von Micrococcus radiodurans in einem System der zellfreien Proteinsynthese by Sussmuth R. (1979)
Vaara , M. , and T. Vaara . 1983 . Sensitization of Gram-negative bacteria to antibiotics and complement by a nontoxic oligopeptide . Nature (London) 303 : 526 - 528 . (10.1038/303526a0) / Nature (London) / Sensitization of Gram-negative bacteria to antibiotics and complement by a nontoxic oligopeptide by Vaara M. (1983)
Vaara , M. , and T. Vaara . 1983 . Polycations sensitize enteric bacteria to antibiotics . Antimicrob. Agents Chemother. 24 : 107 - 113 . (10.1128/AAC.24.1.107) / Antimicrob. Agents Chemother. / Polycations sensitize enteric bacteria to antibiotics by Vaara M. (1983)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 1:07 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:28 p.m.)
Indexed	1 month, 2 weeks ago (July 11, 2025, 6:22 a.m.)
Issued	40 years, 4 months ago (April 1, 1985)
Published	40 years, 4 months ago (April 1, 1985)
Published Print	40 years, 4 months ago (April 1, 1985)

Funders 0

None

BibTeX

@article{Alatossava_1985, title={Manipulation of intracellular magnesium content in polymyxin B nonapeptide-sensitized Escherichia coli by ionophore A23187}, volume={162}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.162.1.413-419.1985}, DOI={10.1128/jb.162.1.413-419.1985}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Alatossava, T and Jütte, H and Kuhn, A and Kellenberger, E}, year={1985}, month=apr, pages={413–419} }

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