Regulatory region of the heat shock-inducible capR (lon) gene: DNA and protein sequences
10.1128/jb.162.1.271-275.1985
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The CapR protein is an ATP hydrolysis-dependent protease as well as a DNA-stimulated ATPase and a nucleic acid-binding protein. The sequences of the 5' end of the capR (lon) gene DNA and N-terminal end of the CapR protein were determined. The sequence of DNA that specifies the N-terminal portion of the CapR protein was identified by comparing the amino acid sequence of the CapR protein with the sequence predicted from the DNA. The DNA and protein sequences established that the mature protein is not processed from a precursor form. No sequence corresponding to an SOS box was found in the 5' sequence of DNA. There were sequences that corresponded to a putative -35 and -10 region for RNA polymerase binding. The capR (lon) gene was recently identified as one of 17 heat shock genes in Escherichia coli that are positively regulated by the product of the htpR gene. A comparison of the 5' DNA region of the capR gene with that of several other heat shock genes revealed possible consensus sequences.

Bibliography

Gayda, R. C., Stephens, P. E., Hewick, R., Schoemaker, J. M., Dreyer, W. J., & Markovitz, A. (1985). Regulatory region of the heat shock-inducible capR (lon) gene: DNA and protein sequences. Journal of Bacteriology, 162(1), 271–275.

Authors 6
  1. R C Gayda (first)
  2. P E Stephens (additional)
  3. R Hewick (additional)
  4. J M Schoemaker (additional)
  5. W J Dreyer (additional)
  6. A Markovitz (additional)
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Dates
Type When
Created 5 years, 7 months ago (Jan. 3, 2020, 11:55 a.m.)
Deposited 2 years, 10 months ago (Oct. 9, 2022, 6:02 p.m.)
Indexed 1 year, 10 months ago (Oct. 25, 2023, 4:18 a.m.)
Issued 40 years, 4 months ago (April 1, 1985)
Published 40 years, 4 months ago (April 1, 1985)
Published Print 40 years, 4 months ago (April 1, 1985)
Funders 0

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@article{Gayda_1985, title={Regulatory region of the heat shock-inducible capR (lon) gene: DNA and protein sequences}, volume={162}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.162.1.271-275.1985}, DOI={10.1128/jb.162.1.271-275.1985}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Gayda, R C and Stephens, P E and Hewick, R and Schoemaker, J M and Dreyer, W J and Markovitz, A}, year={1985}, month=apr, pages={271–275} }