Crossref
journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract
Metabolic changes have been investigated during continuous growth of yeast cells inoculated in glucose-containing medium until the cells entered the stationary phase in response to glucose exhaustion. Well in advance of glucose exhaustion, a transition phase was observed, characterized by a decrease in the growth rate and a progressive reduction of protein and RNA accumulation. Two-dimensional gel analysis of the proteins synthesized during this stage showed that the pattern of proteins remained similar to that of log-phase cells. When the cells entered the stationary phase, protein accumulation was 10% of that in log-phase cells, and incorporation of labeled RNA precursor was undetectable. Analysis of protein synthesis gave evidence that the synthesis of 95% of the proteins present in log-phase cells was arrested in stationary-phase cells. Among the 20 proteins whose synthesis continues throughout the stationary phase were identified actin, aldehyde dehydrogenase, enolase, hexokinase, glyceraldehyde-3-phosphate dehydrogenase, and five heat shock proteins. In addition, the synthesis of six new proteins was observed. The occurrence of these new proteins in stationary-phase cells is presumed to result from the release of carbon catabolite repression due to glucose exhaustion.
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Dates
| Type | When |
|---|---|
| Created | 5 years, 7 months ago (Jan. 3, 2020, 1:04 p.m.) |
| Deposited | 4 years ago (July 29, 2021, 1:36 p.m.) |
| Indexed | 1 year, 1 month ago (July 8, 2024, 1:11 p.m.) |
| Issued | 40 years, 7 months ago (Jan. 1, 1985) |
| Published | 40 years, 7 months ago (Jan. 1, 1985) |
| Published Print | 40 years, 7 months ago (Jan. 1, 1985) |
@article{Boucherie_1985, title={Protein synthesis during transition and stationary phases under glucose limitation in Saccharomyces cerevisiae}, volume={161}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.161.1.385-392.1985}, DOI={10.1128/jb.161.1.385-392.1985}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Boucherie, H}, year={1985}, month=jan, pages={385–392} }