DOI: 10.1128/jb.158.1.362-364.1984. Genetic screen for cloned release factor genes

Genetic screen for cloned release factor genes

10.1128/jb.158.1.362-364.1984

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Nonsense suppression reflects competition between a nonsense suppressor tRNA and a translational release factor. This provides a simple way to screen for release factor genes cloned into a multicopy plasmid. We have confirmed that the expected competition occurs with the gene for release factor 2, cloned by C.T. Caskey et al. (C.T. Caskey, W.C. Forrester, W. Tate, and C.D. Ward, J. Bacteriol. 158:365-368, 1984), and used it to clone the gene for release factor 1.

Bibliography

Weiss, R. B., Murphy, J. P., & Gallant, J. A. (1984). Genetic screen for cloned release factor genes. Journal of Bacteriology, 158(1), 362â364.

Authors 3

R B Weiss (first)
J P Murphy (additional)
J A Gallant (additional)

References 11 Referenced 42

Beaudet , A. L. , and C. T. Caskey . 1970 . Release factor translation of RNA phage terminator codons . Nature (London) 227 : 38 - 40 . (10.1038/227038a0) / Nature (London) / Release factor translation of RNA phage terminator codons by Beaudet A. L. (1970)
Breeden , L. , and M. Yarus . 1982 . Amber suppression relaxes stringent control by elongating stringent factor . Mol. Gen. Genet. 187 : 254 - 264 . 364 NOTES (10.1007/BF00331127) / Mol. Gen. Genet. / Amber suppression relaxes stringent control by elongating stringent factor by Breeden L. (1982)
Capecchi , M. R. , and H. A. Klein . 1969 . Characterization of three proteins involved in polypeptide chaip termination . Cold Spring Harbor Symp. Quapt. Biol. 34 : 469 - 477 . (10.1101/SQB.1969.034.01.053) / Cold Spring Harbor Symp. Quapt. Biol. / Characterization of three proteins involved in polypeptide chaip termination by Capecchi M. R. (1969)
Caskey , T. , E. Scolnick , R. Tompkins , J. Goldstein , and G. Milman . 1969 . Peptide chain termination, codon, protein factor, and ribosomal requirements. Cold SpnrEg Har,bor Symp . Quant. Biol. 34 : 479 - 491 . (10.1101/SQB.1969.034.01.054) / Quant. Biol. / Peptide chain termination, codon, protein factor, and ribosomal requirements. Cold SpnrEg Har,bor Symp by Caskey T. (1969)
Caskey , C. T. , W. ,C. Forrester , W. Tate , and C. D. Ward . 1984 . Cloning of the Escherichia coli release factor 2 gene . J. Bacteriol. 158 : 365 - 336 &. (10.1128/JB.158.1.365-368.1984) / J. Bacteriol. / Cloning of the Escherichia coli release factor 2 gene by Caskey C. T. (1984)
Chang , A. C. Y. , and S. N. Cohen . 1978 . Construction and characterizatiQn' of amplifiable multicopy DNA cloning vehicles derived from the P1SA cryptic miniplasmid . J. Bacteriol. 134 : 1141 - 1156 . (10.1128/JB.134.3.1141-1156.1978) / J. Bacteriol. / Construction and characterizatiQn' of amplifiable multicopy DNA cloning vehicles derived from the P1SA cryptic miniplasmid by Chang A. C. Y. (1978)
Ggllant , J. , H. Erlich , R. Weiss , L. Palmer , and L. Nyari . 1982 . Nonsense suppression in aminoacyl-tRNA limited cells . Mol. Gen. Genet. 186 : 221 - 227 . (10.1007/BF00331853) / Mol. Gen. Genet. / Nonsense suppression in aminoacyl-tRNA limited cells by Ggllant J. (1982)
Horwitz , J. P. , J. Chua , R. J. Curby , A. J. Tomson , M. A. DaRooge , B. E. Fisher , J. Mauricio , and I. Klundt . 1964 . Substrates for cytochemical demo'nstration of enzyme activity. I. Some substituted 3-indolyl-P-D-glycopyianosides . J. Med. Chem. 7 : 574 - 575 . (10.1021/jm00334a044) / J. Med. Chem. / Substrates for cytochemical demo'nstration of enzyme activity. I. Some substituted 3-indolyl-P-D-glycopyianosides by Horwitz J. P. (1964)
Sancar , A. , A. M. Hack , and W. D. Rupp . 1979 . Simple method for identification of plasmid-coded proteins . J. Bacteriol. 137 : 692 - 693 . (10.1128/JB.137.1.692-693.1979) / J. Bacteriol. / Simple method for identification of plasmid-coded proteins by Sancar A. (1979)
Scolnick , E. , R. Tompkins , T. Caskey , and M. Nirenberg . 1968 . Release factors differing in specificity for termination ,odons . Proc. Natl. Acad. Sci. U.S.A. 61 : 768 - 774 . (10.1073/pnas.61.2.768) / Proc. Natl. Acad. Sci. U.S.A. / Release factors differing in specificity for termination ,odons by Scolnick E. (1968)
Struhl , ' K. , D. T. Stinchcomb , S. Scherer , and R. W. Davis . 1979 . High frequency transformation of yeast: autonomous replication of hybrid DNA molecules . Proc. Natl. Acad. Sci. U.S.A. 76 : 1035 - 1039 . (10.1073/pnas.76.3.1035) / Proc. Natl. Acad. Sci. U.S.A. / High frequency transformation of yeast: autonomous replication of hybrid DNA molecules by Struhl K. (1979)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 7:56 a.m.)
Deposited	4 years ago (July 29, 2021, 9:34 a.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:48 p.m.)
Issued	41 years, 4 months ago (April 1, 1984)
Published	41 years, 4 months ago (April 1, 1984)
Published Print	41 years, 4 months ago (April 1, 1984)

Funders 0

None

BibTeX

@article{Weiss_1984, title={Genetic screen for cloned release factor genes}, volume={158}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.158.1.362-364.1984}, DOI={10.1128/jb.158.1.362-364.1984}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Weiss, R B and Murphy, J P and Gallant, J A}, year={1984}, month=apr, pages={362–364} }

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