DOI: 10.1128/jb.154.2.598-603.1983. pH dependence of the Coxiella burnetii glutamate transport system

pH dependence of the Coxiella burnetii glutamate transport system

10.1128/jb.154.2.598-603.1983

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The transport of glutamate, apparently a primary energy source for Coxiella burnetii, has been examined. C. burnetii is shown to possess a pH-dependent active transport system for L-glutamate with an apparent Kt of 61.1 microM and Vmax of 8.33 pmol/s per mg at pH 3.5. Both L-glutamine and L-asparagine competitively inhibited transport of glutamate, but D-glutamate, L-aspartate, L-glutamate-gamma-methyl ester, methionine sulfoximine, or alpha-ketoglutarate did not compete. This transport system is both temperature and energy dependent. Uptake of glutamate is highly sensitive to uncouplers of oxidative phosphorylation such as 2,4-dinitrophenol and carbonyl cyanide-m-chlorophenyl hydrazone that decrease the proton motive force across the cytoplasmic membrane. ATPase inhibitors such as dicyclohexylcarbodiimide or metabolic poisons such as KCN, NaF, or arsenite were much less effective as inhibitors of glutamate transport. Uptake of glutamate did not appear to be coupled to Na+ symport as in Escherichia coli since no monovalent cation requirement could be demonstrated. Instead, the Vmax of glutamate transport showed good correlation with the transmembrane pH gradient (delta pH). From these results, we propose that L-glutamate transport by C. burnetii is energized via a proton motive force.

Bibliography

Hackstadt, T., & Williams, J. C. (1983). pH dependence of the Coxiella burnetii glutamate transport system. Journal of Bacteriology, 154(2), 598â603.

Authors 2

T Hackstadt (first)
J C Williams (additional)

References 18 Referenced 50

Bentaboulet , M. , and A. Kepes . 1981 . Dependence on pH of parameters of lactose transport in Escherichia coli: evidence for an essential protonated group . Eur. J. Biochem. 117 : 233 - 238 . (10.1111/j.1432-1033.1981.tb06327.x) / Eur. J. Biochem. / Dependence on pH of parameters of lactose transport in Escherichia coli: evidence for an essential protonated group by Bentaboulet M. (1981)
Dowd , J. E. , and D. S. Riggs . 1965 . A comparison of estimates of Michaelis-Menten kinetic constants from various linear transformations . J. Biol. Chem. 240 : 863 - 869 . (10.1016/S0021-9258(17)45254-9) / J. Biol. Chem. / A comparison of estimates of Michaelis-Menten kinetic constants from various linear transformations by Dowd J. E. (1965)
Eddy , A. A. 1978 . Proton-dependent solute transport in microorganisms . Curr. Top. Membr. Transp. 10 : 279 - 360 . (10.1016/S0070-2161(08)60837-0) / Curr. Top. Membr. Transp. / Proton-dependent solute transport in microorganisms by Eddy A. A. (1978)
Frank , L. , and I. Hopkins . 1969 . Sodium-stimulated transport of glutamate in Escherichia coli . J. Bacteriol. 100 : 329 - 336 . (10.1128/JB.100.1.329-336.1969) / J. Bacteriol. / Sodium-stimulated transport of glutamate in Escherichia coli by Frank L. (1969)
Hackstadt , T. 1983 . Estimation of the cytoplasmic pH of Coxiella burnetii and effect of substrate oxidation on proton motive force . J. Bacteriol. 154 : 591 - 597 . (10.1128/JB.154.2.591-597.1983) / J. Bacteriol. / Estimation of the cytoplasmic pH of Coxiella burnetii and effect of substrate oxidation on proton motive force by Hackstadt T. (1983)
Hackstadt , T. , and J. C. Williams . 1981 . Biochemical strategem for obligate parasitism of eukaryotic cells by Coxiella burnetii . Proc. Natl. Acad. Sci. U.S.A. 78 : 3240 - 3244 . (10.1073/pnas.78.5.3240) / Proc. Natl. Acad. Sci. U.S.A. / Biochemical strategem for obligate parasitism of eukaryotic cells by Coxiella burnetii by Hackstadt T. (1981)
Hackstadt T. and J. C. Williams. 1981. Incorporation of macromolecular precursors by Coxiella burnetii in an axenic medium p. 431-440. In W. Burgdorfer and R. L. Anacker (ed.) Rickettsiae and rickettsial diseases. Academic Press Inc. New York.
Hackstadt , T. , and J. C. Williams . 1981 . Stability of the adenosine 5'-triphosphate pool in Coxiella burnetii: influence of pH and substrate . J. Bacteriol. 148 : 419 - 425 . (10.1128/jb.148.2.419-425.1981) / J. Bacteriol. / Stability of the adenosine 5'-triphosphate pool in Coxiella burnetii: influence of pH and substrate by Hackstadt T. (1981)
Halpern , Y. S. , and A. Even-Shoshan . 1967 . Properties of the glutamate transport system in Escherichia coli . J. Bacteriol. 93 : 1009 - 1016 . (10.1128/JB.93.3.1009-1016.1967) / J. Bacteriol. / Properties of the glutamate transport system in Escherichia coli by Halpern Y. S. (1967)
Harold , F. M. 1972 . Conservation and transformation of energy by bacterial membranes . Bacteriol. Rev. 36 : 172 - 230 . (10.1128/br.36.2.172-230.1972) / Bacteriol. Rev. / Conservation and transformation of energy by bacterial membranes by Harold F. M. (1972)
Harold , F. M. 1977 . Membranes and energy transduction in bacteria . Curr. Top. Bioenerg. 6 : 83 - 149 . / Curr. Top. Bioenerg. / Membranes and energy transduction in bacteria by Harold F. M. (1977)
Lanyi , J. K. 1979 . The role of Na+ in transport processes of bacterial membranes . Biochim. Biophys. Acta 559 : 377 - 397 . (10.1016/0304-4157(79)90011-X) / Biochim. Biophys. Acta / The role of Na+ in transport processes of bacterial membranes by Lanyi J. K. (1979)
Lehninger A. L. 1970. Biochemistry. Worth Publishers Inc. New York.
MacDonald , R. E. , J. K. Lanyl , and R. V. Greene . 1977 . Sodium-stimulated glutamate uptake in membrane vesicles of Escherichia coli: the role of ion gradients . Proc. Natl. Acad. Sci. U.S.A. 74 : 3167 - 3170 . (10.1073/pnas.74.8.3167) / Proc. Natl. Acad. Sci. U.S.A. / Sodium-stimulated glutamate uptake in membrane vesicles of Escherichia coli: the role of ion gradients by MacDonald R. E. (1977)
Povalla , K. , E. Kannenberg , and A. Blume . 1980 . A glycolipid containing hopane isolated from the acidophilic, thermophilic Bacillus acidocaldarius, has a cholesterol-like function in membranes . FEBS Lett. 113 : 107 - 110 . (10.1016/0014-5793(80)80506-0) / FEBS Lett. / A glycolipid containing hopane isolated from the acidophilic, thermophilic Bacillus acidocaldarius, has a cholesterol-like function in membranes by Povalla K. (1980)
Schultz , S. G. , and P. F. Curran . 1970 . Coupled transport of sodium and organic solutes . Physiol. Rev. 50 : 637 - 718 . (10.1152/physrev.1970.50.4.637) / Physiol. Rev. / Coupled transport of sodium and organic solutes by Schultz S. G. (1970)
Tsuchiya , T. , S. M. Hasan , and, J. Raven . 1977 . Glutamate transport driven by an electrochemical gradient of sodium ions in Escherichia coli . J. Bacteriol. 131 : 848 - 853 . (10.1128/JB.131.3.848-853.1977) / J. Bacteriol. / Glutamate transport driven by an electrochemical gradient of sodium ions in Escherichia coli by Tsuchiya T. (1977)
Wiliams , J. C. , M. G. Peacock , and T. F. McCaul . 1981 . Immunological and biological characterization of Coxiella burnetii, phases I and II, separated from host components . Infect. Immun. 32 : 840 - 851 . (10.1128/IAI.32.2.840-851.1981) / Infect. Immun. / Immunological and biological characterization of Coxiella burnetii, phases I and II, separated from host components by Wiliams J. C. (1981)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 12:42 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:31 p.m.)
Indexed	2 months, 1 week ago (June 25, 2025, 5:43 p.m.)
Issued	42 years, 4 months ago (May 1, 1983)
Published	42 years, 4 months ago (May 1, 1983)
Published Print	42 years, 4 months ago (May 1, 1983)

Funders 0

None

BibTeX

@article{Hackstadt_1983, title={pH dependence of the Coxiella burnetii glutamate transport system}, volume={154}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.154.2.598-603.1983}, DOI={10.1128/jb.154.2.598-603.1983}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Hackstadt, T and Williams, J C}, year={1983}, month=may, pages={598–603} }

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