DOI: 10.1128/jb.154.2.569-572.1983. Genetic mapping of the Escherichia coli leader (signal) peptidase gene (lep): a new approach for determining the map position of a cloned gene

Genetic mapping of the Escherichia coli leader (signal) peptidase gene (lep): a new approach for determining the map position of a cloned gene

10.1128/jb.154.2.569-572.1983

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The gene for leader peptidase, termed lep, was mapped to the region between purI and nadB at min 54 to 55 on the Escherichia coli chromosome. Mapping involved (i) cloning the gene into the plasmid pBR322, (ii) transforming the plasmid into a polA strain where it cannot replicate autonomously, (iii) selecting by ampicillin resistance the rare cell in which the plasmid had recombined into the chromosome, and (iv) mapping the chromosomal site of drug resistance (and thus plasmid integration) by Hfr matings and P1 transduction. The map position was confirmed by an assay of the enzyme content of cells bearing an F' factor which covered that region of the chromosome.

Bibliography

Silver, P., & Wickner, W. (1983). Genetic mapping of the Escherichia coli leader (signal) peptidase gene (lep): a new approach for determining the map position of a cloned gene. Journal of Bacteriology, 154(2), 569â572.

Authors 2

P Silver (first)
W Wickner (additional)

References 10 Referenced 45

abn, B. J., and K. B. Low . 1980 . Linkage map of Escherichia coli K-12, edition 6 . Microbiol. Rev. 44 : 1 - 56 . (10.1128/MR.44.1.1-56.1980) / Microbiol. Rev. / Linkage map of Escherichia coli K-12, edition 6 by Low (1980)
Clarke , L. , and J. Carbon . 1976 . A colony bank containing synthetic ColEl hybrid plasmids representative of the entire E. coli genome . Cell 9 : 91 - 99 . (10.1016/0092-8674(76)90055-6) / Cell / A colony bank containing synthetic ColEl hybrid plasmids representative of the entire E. coli genome by Clarke L. (1976)
Date , T. , and W. Wkkner . 1981 . Isolation of the Escherichia coli leader peptidase gene and effects of leader peptidase overproduction in vivo . Proc. Natl. Acad. Sci. U.S.A. 78 : 6106 - 6110 . (10.1073/pnas.78.10.6106) / Proc. Natl. Acad. Sci. U.S.A. / Isolation of the Escherichia coli leader peptidase gene and effects of leader peptidase overproduction in vivo by Date T. (1981)
Hawrot , E. , and E. P. Kenody . 1975 . Biogenesis of membrane lipids: mutants of Escherichia coli with temperature sensitive phosphatidylserine decarboxylase . Proc. Natl. Acad. Sci. U.S.A. 72 : 1112 - 1116 . (10.1073/pnas.72.3.1112) / Proc. Natl. Acad. Sci. U.S.A. / Biogenesis of membrane lipids: mutants of Escherichia coli with temperature sensitive phosphatidylserine decarboxylase by Hawrot E. (1975)
KI u_ry, D. T., and D. R. Helnsl . 1970 . DNA polymerase as a requirement for the maintenance of the bacterial pasnmid colicinogenic factor E1 . Biochem. Biophys. Res. Commun. 41 : 1538 - 1544 . (10.1016/0006-291X(70)90562-0) / Biochem. Biophys. Res. Commun. / DNA polymerase as a requirement for the maintenance of the bacterial pasnmid colicinogenic factor E1 by Helnsl (1970)
MIller J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
Soutdern , E. M. 1975 . Detection of specific sequences among DNA fragments separated by gel electrophoresis . J. Mol. Biol. 98 : 503 - 517 . (10.1016/S0022-2836(75)80083-0) / J. Mol. Biol. / Detection of specific sequences among DNA fragments separated by gel electrophoresis by Soutdern E. M. (1975)
Wolfe , P. , P. Silver , and W. Wlkner . 1982 . The isolation of homogeneous leader peptidase from a strain of Escherichia coll which overproduces the enzyme . J. Biol. Chem. 257 : 7898 - 7902 . (10.1016/S0021-9258(18)34466-1) / J. Biol. Chem. / The isolation of homogeneous leader peptidase from a strain of Escherichia coll which overproduces the enzyme by Wolfe P. (1982)
Yamnaguch , K. , and J. Towinwa . 1980 . Establishment of Escherichia coli cells with an integrated high copy number plasmid . Mol. Gen. Genet. 178 : 525 - 533 . (10.1007/BF00337857) / Mol. Gen. Genet. / Establishment of Escherichia coli cells with an integrated high copy number plasmid by Yamnaguch K. (1980)
Zwlzadl , C. , and W. Wlkner . 1980 . Purification and characterization of leader (signal) peptidase from Escherichia coli . J. Biol. Chem. 255 : 7973 - 7977 . (10.1016/S0021-9258(19)43928-8) / J. Biol. Chem. / Purification and characterization of leader (signal) peptidase from Escherichia coli by Zwlzadl C. (1980)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 11:45 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:31 p.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 4:39 p.m.)
Issued	42 years, 4 months ago (May 1, 1983)
Published	42 years, 4 months ago (May 1, 1983)
Published Print	42 years, 4 months ago (May 1, 1983)

Funders 0

None

BibTeX

@article{Silver_1983, title={Genetic mapping of the Escherichia coli leader (signal) peptidase gene (lep): a new approach for determining the map position of a cloned gene}, volume={154}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.154.2.569-572.1983}, DOI={10.1128/jb.154.2.569-572.1983}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Silver, P and Wickner, W}, year={1983}, month=may, pages={569–572} }

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