DOI: 10.1128/jb.153.1.543-545.1983. In Vitro Synthesis of Subunits of Bacterial Luciferase in an <i>Escherichia coli</i> System

In Vitro Synthesis of Subunits of Bacterial Luciferase in an Escherichia coli System

10.1128/jb.153.1.543-545.1983

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

RNA extracted from the luminescent bacterium Vibrio harveyi was translated in an Escherichia coli system. RNA from highly luminescent cells produced both α and β subunits of luciferase in vitro, as confirmed by immunoprecipitation and partial proteolysis. RNA from cells before induction of luminescence did not direct luciferase synthesis.

Bibliography

Evans, J. F., McCracken, S., Miyamoto, C. M., Meighen, E. A., & Graham, A. F. (1983). In Vitro Synthesis of Subunits of Bacterial Luciferase in an Escherichia coli System. Journal of Bacteriology, 153(1), 543â545.

Authors 5

Jillian F. Evans (first)
Susan McCracken (additional)
Carol M. Miyamoto (additional)
Edward A. Meighen (additional)
Angus F. Graham (additional)

References 12 Referenced 12

Asana , K. 1965 . Size heterogeneity of T2 messenger RNA . J. Mol. Biol. 14 : 71 - 84 . (10.1016/S0022-2836(65)80231-5) / J. Mol. Biol. / Size heterogeneity of T2 messenger RNA by Asana K. (1965)
Bottomley , W. , and P. R. Whitfield . 1979 . Cell-free transcription and translation of total spinach chloroplast DNA . Eur. J. Biochem. 93 : 31 - 39 . (10.1111/j.1432-1033.1979.tb12791.x) / Eur. J. Biochem. / Cell-free transcription and translation of total spinach chloroplast DNA by Bottomley W. (1979)
Cleveland , D. W. , S. G. Flscher , M. W. Kirschner , and U. K. Laemmli . 1977 . Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis . J. Biol. Chem. 252 : 1102 - 1106 . (10.1016/S0021-9258(19)75212-0) / J. Biol. Chem. / Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis by Cleveland D. W. (1977)
Holmes , W. M. , G. W. Hatfield , and E. Goldman . 1978 . Evidence for misreading during tRNA-dependent protein synthesis in vitro . J. Biol. Chem. 253 : 3483 - 3486 . (10.1016/S0021-9258(17)34826-3) / J. Biol. Chem. / Evidence for misreading during tRNA-dependent protein synthesis in vitro by Holmes W. M. (1978)
Kempner , E. S. , and F. E. Hanson . 1968 . Aspects of light production of Photobacterium fischeri . J. Bacteriol. 95 : 975 - 979 . (10.1128/jb.95.3.975-979.1968) / J. Bacteriol. / Aspects of light production of Photobacterium fischeri by Kempner E. S. (1968)
Laemmli , U. K. 1970 . Cleavage of structural proteins during the assembly of the head of bacteriophage T4 . Nature (London) 227 : 680 - 685 . (10.1038/227680a0) / Nature (London) / Cleavage of structural proteins during the assembly of the head of bacteriophage T4 by Laemmli U. K. (1970)
Mlchaliszyn , G. A. , and E. A. Meighen . 1976 . Induced polypeptide synthesis during the development of bacterial bioluminescence . J. Biol. Chem. 251 : 2541 - 2549 . (10.1016/S0021-9258(17)33522-6) / J. Biol. Chem. / Induced polypeptide synthesis during the development of bacterial bioluminescence by Mlchaliszyn G. A. (1976)
Nathans , D. , G. Notani , J. H. Schwartz , and N. D. Zinder . 1962 . Biosynthesis of the coat protein of coliphage f2 by E. coli extracts . Proc. Natl. Acad. Sci. U.S.A. 48 : 1424 - 1431 . (10.1073/pnas.48.8.1424) / Proc. Natl. Acad. Sci. U.S.A. / Biosynthesis of the coat protein of coliphage f2 by E. coli extracts by Nathans D. (1962)
Nealson , K. H. , T. Platt , J. W. Hastins . 1970 . Cellular control of the synthesis and activity of the bacterial luminescent system . J. Bacteriol. 104 : 313 - 322 . (10.1128/jb.104.1.313-322.1970) / J. Bacteriol. / Cellular control of the synthesis and activity of the bacterial luminescent system by Nealson K. H. (1970)
Neeman , Z. , S. Ulitzur , D. Branton , and J. W. Hastings . 1977 . Membrane polypeptides co-induced with the bacterial bioluminescent system . J. Biol. Chem. 252 : 5150 - 5154 . (10.1016/S0021-9258(17)40169-4) / J. Biol. Chem. / Membrane polypeptides co-induced with the bacterial bioluminescent system by Neeman Z. (1977)
Palmiter , R. D. , 0. Takami , and R. T. Schimke . 1971 . Modulation of ovalbumin synthesis by estradio1-17B and actinomycin D as studied in explants of chick oviduct in culture . J. Biol. Chem. 246 : 724 - 737 . (10.1016/S0021-9258(18)62473-1) / J. Biol. Chem. / Modulation of ovalbumin synthesis by estradio1-17B and actinomycin D as studied in explants of chick oviduct in culture by Palmiter R. D. (1971)
Whitfeld , P. R. , and W. Bottomley . 1980 . Mapping of the gene for the large subunit of ribulose biphosphate carboxylase on spinach chloroplast DNA . Biochem. Int. 1 : 172 - 178 . / Biochem. Int. / Mapping of the gene for the large subunit of ribulose biphosphate carboxylase on spinach chloroplast DNA by Whitfeld P. R. (1980)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 12:40 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:29 p.m.)
Indexed	1 year, 10 months ago (Oct. 19, 2023, 10:29 p.m.)
Issued	42 years, 8 months ago (Jan. 1, 1983)
Published	42 years, 8 months ago (Jan. 1, 1983)
Published Print	42 years, 8 months ago (Jan. 1, 1983)

Funders 0

None

BibTeX

@article{Evans_1983, title={In Vitro Synthesis of Subunits of Bacterial Luciferase in an Escherichia coli System}, volume={153}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.153.1.543-545.1983}, DOI={10.1128/jb.153.1.543-545.1983}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Evans, Jillian F. and McCracken, Susan and Miyamoto, Carol M. and Meighen, Edward A. and Graham, Angus F.}, year={1983}, month=jan, pages={543–545} }

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