DOI: 10.1128/jb.153.1.163-168.1983. Transformation of intact yeast cells treated with alkali cations

Transformation of intact yeast cells treated with alkali cations

10.1128/jb.153.1.163-168.1983

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Intact yeast cells treated with alkali cations took up plasmid DNA. Li+, Cs+, Rb+, K+, and Na+ were effective in inducing competence. Conditions for the transformation of Saccharomyces cerevisiae D13-1A with plasmid YRp7 were studied in detail with CsCl. The optimum incubation time was 1 h, and the optimum cell concentration was 5 x 10(7) cells per ml. The optimum concentration of Cs+ was 1.0 M. Transformation efficiency increased with increasing concentrations of plasmid DNA. Polyethylene glycol was absolutely required. Heat pulse and various polyamines or basic proteins stimulated the uptake of plasmid DNA. Besides circular DNA, linear plasmid DNA was also taken up by Cs+-treated yeast cells, although the uptake efficiency was considerably reduced. The transformation efficiency with Cs+ or Li+ was comparable with that of conventional protoplast methods for a plasmid containing ars1, although not for plasmids containing a 2 microns origin replication.

Bibliography

Ito, H., Fukuda, Y., Murata, K., & Kimura, A. (1983). Transformation of intact yeast cells treated with alkali cations. Journal of Bacteriology, 153(1), 163â168.

Authors 4

H Ito (first)
Y Fukuda (additional)
K Murata (additional)
A Kimura (additional)

References 16 Referenced 5,531

Beach , D. , and P. Nurse . 1981 . High-frequency transformation of the fission yeast Schizosaccharomyces pombe . Nature (London) 290 : 140 - 142 . (10.1038/290140a0) / Nature (London) / High-frequency transformation of the fission yeast Schizosaccharomyces pombe by Beach D. (1981)
Cameron , J. R. , P. Phillppsen , and R. W. Davis . 1977 . Analysis of chromosomal integration and deletions of yeast plasmids . Nucleic Acids Res. 4 : 1429 - 1448 . (10.1093/nar/4.5.1429) / Nucleic Acids Res. / Analysis of chromosomal integration and deletions of yeast plasmids by Cameron J. R. (1977)
Ferenczy , L. , and A. Maraz . 1977 . Transfer of mitochondria by protoplast fusion in Saccharomyces cerevisiae . Nature (London) 268 : 524 - 525 . (10.1038/268524a0) / Nature (London) / Transfer of mitochondria by protoplast fusion in Saccharomyces cerevisiae by Ferenczy L. (1977)
Grhdus , L. 1980 . Nucleic acid transport driven by ion gradient across cell membrane . FEBS Lett. 113 : 1 - 10 . (10.1016/0014-5793(80)80482-0) / FEBS Lett. / Nucleic acid transport driven by ion gradient across cell membrane by Grhdus L. (1980)
HelUng , R. B. , H. M. Goodman , and H. W. Boyer . 1974 . Analysis of endonuclease R * EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis . J. Virol. 14 : 1235 - 1244 . (10.1128/JVI.14.5.1235-1244.1974) / J. Virol. / Analysis of endonuclease R * EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis by HelUng R. B. (1974)
Hlnnen , A. , J. B. Hicks , and G. R. Flnk . 1978 . Transformation of yeast . Proc. Natl. Acad. Sci. U.S.A. 75 : 1929 - 1933 . (10.1073/pnas.75.4.1929) / Proc. Natl. Acad. Sci. U.S.A. / Transformation of yeast by Hlnnen A. (1978)
Kao , K. N. , and M. R. Mlchayluk . 1974 . A method for high frequency intergeneric fusion of plant protoplasts . Planta 115 : 355 - 367 . (10.1007/BF00388618) / Planta / A method for high frequency intergeneric fusion of plant protoplasts by Kao K. N. (1974)
Kimura , A. , K. Arima , and K. Murata . 1981 . Biofunctional change in yeast cell surface on treatment with Triton X-100 . Agric. Biol. Chem. 45 : 2627 - 2629 . / Agric. Biol. Chem. / Biofunctional change in yeast cell surface on treatment with Triton X-100 by Kimura A. (1981)
Kimura , A. , and M. Morita . 1975 . Fermentative formation of CDP-choline by intact cells of yeast: Saccharomyces carlsbergen.sis (IFO 0641) treated with a detergent, Triton X-100 . Agric. Biol. Chem. 39 : 1469 - 1474 . / Agric. Biol. Chem. / Fermentative formation of CDP-choline by intact cells of yeast: Saccharomyces carlsbergen.sis (IFO 0641) treated with a detergent, Triton X-100 by Kimura A. (1975)
Mandel , M. , and A. Hig . 1970 . Calcium-dependent bacteriophage DNA infection . J. Mol. Biol. 53 : 159 - 162 . (10.1016/0022-2836(70)90051-3) / J. Mol. Biol. / Calcium-dependent bacteriophage DNA infection by Mandel M. (1970)
Meyers , J. A. , D. Sanchez , L. P. Elwell , ad S. Falkow . 1976 . Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid . J. Bacteriol. 127 : 1529 - 1537 . (10.1128/JB.127.3.1529-1537.1976) / J. Bacteriol. / Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid by Meyers J. A. (1976)
Naharl K. 1979. DNA kumikae gijutsu p. 162-163. In K. Sakaguchi and M. Okanishi (ed.) Bunshi ikushu to oyo biseibtsu. Kodansha Tokyo. (In Japanese.)
Struhl ,d, D.T. Dhdecmb, S. Scherer , and R. W. Davis . 1979 . High-frequwecy transformation of yeast: autonomous replication 'f hybrid DNA molecules . Proc. Natl. Acad. Sci. U.S.A. 75 : 1929 - 1933 . / Proc. Natl. Acad. Sci. U.S.A. / High-frequwecy transformation of yeast: autonomous replication 'f hybrid DNA molecules by Struhl D.T. (1979)
Sinkl M. ad I. Tanabe. 1976. Uptake of single-strand bacteriophage DNA by isolated tobacco protoplasts. Z. PManzenphysiol. 7&21-433.
va Sollngen ,- P. , aig L. B. van der Plaat . 1977 . Fusion of yeast spheroplasts . J. Bacteriol. 130 : 946 - 947 . (10.1128/JB.130.2.946-947.1977) / J. Bacteriol. / Fusion of yeast spheroplasts by Sollngen P. (1977)
Yanofsky , C. , V. Horn , M. Bonner , and S. Ttasiowskl . 1971 . Polrity and enzyme functions in mutants of the first three genes of the tryptophan operon of Escherichia coli . Genetics 69 : 409 - 433 . (10.1093/genetics/69.4.409) / Genetics / Polrity and enzyme functions in mutants of the first three genes of the tryptophan operon of Escherichia coli by Yanofsky C. (1971)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 12:40 p.m.)
Deposited	4 years ago (July 29, 2021, 1:29 p.m.)
Indexed	1 day, 16 hours ago (Aug. 21, 2025, 12:42 p.m.)
Issued	42 years, 7 months ago (Jan. 1, 1983)
Published	42 years, 7 months ago (Jan. 1, 1983)
Published Print	42 years, 7 months ago (Jan. 1, 1983)

Funders 0

None

BibTeX

@article{Ito_1983, title={Transformation of intact yeast cells treated with alkali cations}, volume={153}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.153.1.163-168.1983}, DOI={10.1128/jb.153.1.163-168.1983}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Ito, H and Fukuda, Y and Murata, K and Kimura, A}, year={1983}, month=jan, pages={163–168} }

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