Bioenergetic properties and viability of alkalophilic Bacillus firmus RAB as a function of pH and Na+ contents of the incubation medium
10.1128/jb.152.3.1096-1104.1982
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The bioenergetic properties and viability of obligately alkalophilic Bacillus firmus RAB have been examined upon incubation in alkaline and neutral buffers in the presence or absence of added Na+. At pH 10.5, cells incubated in the absence of Na+ exhibited an immediate rise in cytoplasmic pH from less than 9.5 to 10.5, and they lost viability very rapidly. Viability experiments in the presence or absence of an energy source further suggested that the Na+-dependent mechanism for pH homeostasis is an energy-requiring function. The Na+/H+ antiporter, which catalyzes the vital proton accumulation at alkaline pH, was only slightly operational at pH 7.0; both whole cells and vesicles exhibited net proton extrusion even in the presence of Na+. Moreover, cells incubated in buffer at pH 7.0 were actually more viable in the presence of Na+ than in its absence. Thus, the inability of B. firmus RAB to grow at neutral pH is not due to excessive acidification of the cytoplasm. Rather, the transmembrane electrical potential, delta psi, generated at pH 7.0 was found to be much lower than at alkaline pH. The very low delta psi compromised several cell functions, e.g., Na+/solute symport and motility, which in this and other alkalophiles specifically depend upon delta psi and Na+.

Bibliography

Kitada, M., Guffanti, A. A., & Krulwich, T. A. (1982). Bioenergetic properties and viability of alkalophilic Bacillus firmus RAB as a function of pH and Na+ contents of the incubation medium. Journal of Bacteriology, 152(3), 1096–1104.

Authors 3
  1. M Kitada (first)
  2. A A Guffanti (additional)
  3. T A Krulwich (additional)
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Dates
Type When
Created 4 years, 2 months ago (June 2, 2021, 6:33 p.m.)
Deposited 4 years, 2 months ago (June 2, 2021, 6:34 p.m.)
Indexed 1 year, 9 months ago (Nov. 23, 2023, 3:21 a.m.)
Issued 42 years, 9 months ago (Dec. 1, 1982)
Published 42 years, 9 months ago (Dec. 1, 1982)
Published Print 42 years, 9 months ago (Dec. 1, 1982)
Funders 0

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@article{Kitada_1982, title={Bioenergetic properties and viability of alkalophilic Bacillus firmus RAB as a function of pH and Na+ contents of the incubation medium}, volume={152}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.152.3.1096-1104.1982}, DOI={10.1128/jb.152.3.1096-1104.1982}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Kitada, M and Guffanti, A A and Krulwich, T A}, year={1982}, month=dec, pages={1096–1104} }