Genetic and biochemical characterization of periplasmic-leaky mutants of Escherichia coli K-12
10.1128/jb.145.3.1351-1358.1981
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Periplasmic-leaky mutants of Escherichia coli K-12 were isolated after nitrosoguanidine-induced mutagenesis. They released periplasmic enzymes into the extracellular medium. Excretion of alkaline phosphatase, which started immediately in the early exponential phase of growth, could reach up to 90% of the total enzyme production in the stationary phase. Leaky mutants were sensitive to ethylenediaminetetraacetic acid, cholic acid, and the antibiotics rifampin, chloramphenicol, mitomycin C, and ampicillin. Furthermore, they were resistant to colicin E1 and partially resistant to phage TuLa. Their genetic characterization showed that the lky mutations mapped between the suc and gal markers, near or in the tolPAB locus. A biochemical analysis of cell envelope components showed that periplasmic-leaky mutants contained reduced amounts of major outer membrane protein OmpF and increased amounts of a 16,000-dalton outer membrane protein.

Bibliography

Lazzaroni, J. C., & Portalier, R. C. (1981). Genetic and biochemical characterization of periplasmic-leaky mutants of Escherichia coli K-12. Journal of Bacteriology, 145(3), 1351–1358.

Authors 2
  1. J C Lazzaroni (first)
  2. R C Portalier (additional)
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Dates
Type When
Created 5 years, 7 months ago (Jan. 3, 2020, 12:18 p.m.)
Deposited 4 years ago (July 29, 2021, 1:23 p.m.)
Indexed 2 months, 2 weeks ago (June 12, 2025, 3:31 a.m.)
Issued 44 years, 5 months ago (March 1, 1981)
Published 44 years, 5 months ago (March 1, 1981)
Published Print 44 years, 5 months ago (March 1, 1981)
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@article{Lazzaroni_1981, title={Genetic and biochemical characterization of periplasmic-leaky mutants of Escherichia coli K-12}, volume={145}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.145.3.1351-1358.1981}, DOI={10.1128/jb.145.3.1351-1358.1981}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Lazzaroni, J C and Portalier, R C}, year={1981}, month=mar, pages={1351–1358} }