DOI: 10.1128/jb.145.1.74-81.1981. Plasmid transfer and genetic recombination by protoplast fusion in staphylococci

Plasmid transfer and genetic recombination by protoplast fusion in staphylococci

10.1128/jb.145.1.74-81.1981

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The experimental conditions for plasmid transfer and genetic recombination in Staphylococcus aureus and some coagulase-negative staphylococci by protoplast fusion are described. Protoplasts were prepared by treatment with lysostaphin and lysozyme in a buffered medium with 0.7 to 0.8 M sucrose. Regeneration of cell walls was accomplished on a hypertonic agar medium containing succinate and bovine serum albumin. Transfer of plasmids occurred after treatment of the protoplast mixtures with polyethylene glycol (molecular weight, 6,000) not only between strains of the same species but also between parents of different species, although at approximately 100 times lower frequency in the latter case. Recombination of the chromosomal genes in fused protoplasts required simultaneous treatment of the mixed protoplasts with polyethylene glycol and CaCl2. A method was developed for isolation of recombinants after fusion between mutants of S. areus carrying unselectable markers. Antibiotic resistance plasmids were introduced into the parental strains and used as primary markers to detect protoplast fusion. Chromosomal recombinants were found among the clones with both parental plasmids at a high frequency. The method appears to have simple applications in the construction of strains with multiple mutant characters.

Bibliography

GÃ¶tz, F., AhrnÃ©, S., & Lindberg, M. (1981). Plasmid transfer and genetic recombination by protoplast fusion in staphylococci. Journal of Bacteriology, 145(1), 74â81.

Authors 3

F Götz (first)
S Ahrné (additional)
M Lindberg (additional)

References 32 Referenced 76

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Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 12:14 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:22 p.m.)
Indexed	1 year, 9 months ago (Nov. 20, 2023, 4:24 p.m.)
Issued	44 years, 8 months ago (Jan. 1, 1981)
Published	44 years, 8 months ago (Jan. 1, 1981)
Published Print	44 years, 8 months ago (Jan. 1, 1981)

Funders 0

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BibTeX

@article{G_tz_1981, title={Plasmid transfer and genetic recombination by protoplast fusion in staphylococci}, volume={145}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.145.1.74-81.1981}, DOI={10.1128/jb.145.1.74-81.1981}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Götz, F and Ahrné, S and Lindberg, M}, year={1981}, month=jan, pages={74–81} }

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