DOI: 10.1128/jb.142.1.1-7.1980. Characterization of different plasmid-borne dihydropteroate synthases mediating bacterial resistance to sulfonamides

Characterization of different plasmid-borne dihydropteroate synthases mediating bacterial resistance to sulfonamides

10.1128/jb.142.1.1-7.1980

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Plasmid-borne resistance to sulfonamides was studied in both newly isolated and earlier characterized R plasmids. Two different classes of drug-resistant dihydropteroate synthases were found to be responsible for most cases of plasmid-mediated sulfonamide resistance. The plasmid-coded enzymes could be completely separated from their chromosomal counterpart and also showed differences in heat stability and molecular size. The resistant and chromosomal enzymes could bind the normal substrate, p-aminobenzoic acid, with equal efficiency. In contrast, sulfonamide binding was about 10,000 times lower with the plasmid-coded enzymes than with the chromosomal enzyme. Another substrate analog, p-aminosalicylic acid, on the other hand, inhibited chromosomal and plasmid-mediated enzymes to a similar extent. Evidence was also found for the existence of a plasmid-borne resistance mechanism independent of drug-insensitive enzymes.

Bibliography

Swedberg, G., & SkÃ¶ld, O. (1980). Characterization of different plasmid-borne dihydropteroate synthases mediating bacterial resistance to sulfonamides. Journal of Bacteriology, 142(1), 1â7.

Authors 2

G Swedberg (first)
O Sköld (additional)

References 18 Referenced 43

PLASMID-BORNE DIHYDROPTEROATE SYNTHASES
Datta , N. , and R. W. Hedges . 1972 . Trimethoprim resistance conferred by W plasmids in Enterobacteriaceae . J. Gen. Microbiol. 72 : 349 - 355 . (10.1099/00221287-72-2-349) / J. Gen. Microbiol. / Trimethoprim resistance conferred by W plasmids in Enterobacteriaceae by Datta N. (1972)
Edlin , G. 1965 . Gene regulation during bacteriophage T4 development. I. Phenotype reversion of T4 amber mutants by 5-fluorouracil . J. Mol. Biol. 12 : 363 - 374 . (10.1016/S0022-2836(65)80260-1) / J. Mol. Biol. / Gene regulation during bacteriophage T4 development. I. Phenotype reversion of T4 amber mutants by 5-fluorouracil by Edlin G. (1965)
Edlin , G. , and 0. Maaloe . 1966 . Synthesis and breakdown of messenger RNA without protein synthesis . J. Mol. Biol. 15 : 428 - 434 . (10.1016/S0022-2836(66)80118-3) / J. Mol. Biol. / Synthesis and breakdown of messenger RNA without protein synthesis by Edlin G. (1966)
Fraser , D. , and E. A. Jerrel . 1953 . Amino acid composition of T3 bacteriophage . J. Biol. Chem. 205 : 291 - 295 . (10.1016/S0021-9258(19)77254-8) / J. Biol. Chem. / Amino acid composition of T3 bacteriophage by Fraser D. (1953)
Grinsted , J. , J. R. Saunders , L C . Ingram , R. B. Sykes , and M. IL Richmond . 1972 . Properties of an R-factor which originated in Pseudomonas aeruginosa 1822 . J. Bacteriol. 110 : 529 - 537 . (10.1128/jb.110.2.529-537.1972) / J. Bacteriol. / Properties of an R-factor which originated in Pseudomonas aeruginosa 1822 by Grinsted J. (1972)
Kopecko , D. J. , J. Brevet , and S. N. Cohen . 1976 . Involvement of multiple translocating DNA segments and recombinational hotepots in the structural evolution of bacterial plasmids . J. Mol. Biol. 108 : 333 - 360 . (10.1016/S0022-2836(76)80124-6) / J. Mol. Biol. / Involvement of multiple translocating DNA segments and recombinational hotepots in the structural evolution of bacterial plasmids by Kopecko D. J. (1976)
Lowry , 0. H. , N. J. Rosebrough , A. L Farr , and R. J. Randall . 1951 . Protein measurement with the Folin phenol reagent . J. Biol. Chem. 193 : 266 - 275 . (10.1016/S0021-9258(19)52451-6) / J. Biol. Chem. / Protein measurement with the Folin phenol reagent by Lowry 0. (1951)
Meynell , E. , and N. Datta . 1967 . Mutant drug resistant factor of high tranmibility . Nature (London) 214 : 885 - 887 . (10.1038/214885a0) / Nature (London) / Mutant drug resistant factor of high tranmibility by Meynell E. (1967)
Nagate , T. , M. Inoue , K. Inoue , and S. Mitauhashi . 1978 . Plasmid-mediated sulfanilamide resistance . Microbiol. Immunol. 22 : 367 - 375 . (10.1111/j.1348-0421.1978.tb00383.x) / Microbiol. Immunol. / Plasmid-mediated sulfanilamide resistance by Nagate T. (1978)
Richey D. P. and G. M. Brown. 1969. The biosynthesis of folic acid. IX. Purification and properties of the (10.1016/S0021-9258(18)91799-0)
Shiota , T. , C. M. Baugh , R. Jackson , and R. Dullard . 1969 . The enzymatic synthesis of hydrozymethyldihydropteridine pyrophosphate and dihydrofolate . Biochemistry 8 : 5022 - 5028 . (10.1021/bi00840a052) / Biochemistry / The enzymatic synthesis of hydrozymethyldihydropteridine pyrophosphate and dihydrofolate by Shiota T. (1969)
Shiota , T. , M. N. Disraely , and M. P. McCann . 1964 . The enzymatic synthesis of folate-like compounds form hydrozymethyldihydropteridine pyrophosphate . J. Biol. Chem. 239 : 2259 - 2266 . (10.1016/S0021-9258(20)82229-7) / J. Biol. Chem. / The enzymatic synthesis of folate-like compounds form hydrozymethyldihydropteridine pyrophosphate by Shiota T. (1964)
Skold , 0. 1976 . R-factor-mediated resistance to sulfonamides by a plasmid-borne, drug-resistant dihydropteroate synthase . Antimicrob. Agents Chemother. 9 : 49 - 54 . (10.1128/AAC.9.1.49) / Antimicrob. Agents Chemother. / R-factor-mediated resistance to sulfonamides by a plasmid-borne, drug-resistant dihydropteroate synthase by Skold 0. (1976)
Swedberg , G. , S. Casteneson , and 0. Skold . 1979 . Characterization of mutationally altered dihydropteroate synthase and its ability to form a sulfonamidecontaining dihydrofolate analog . J. Bacteriol. 137 : 129 - 136 . (10.1128/JB.137.1.129-136.1979) / J. Bacteriol. / Characterization of mutationally altered dihydropteroate synthase and its ability to form a sulfonamidecontaining dihydrofolate analog by Swedberg G. (1979)
Tennhamsar -l.kman, B. , and 0. Skold . 1979 . Trimethoprim resistance plasmids of different origin encode different drug-resistant dihydrofolate reductases . Plasmid 2 : 334 - 346 . (10.1016/0147-619X(79)90017-9) / Plasmid / Trimethoprim resistance plasmids of different origin encode different drug-resistant dihydrofolate reductases by Tennhamsar B. (1979)
Timmis , K. , F. Cabello , and S. N. Cohen . 1978 . Cloning and characterization of EcoRI and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6 . Mol. Gen. Genet. 162 : 121 - 137 . (10.1007/BF00267869) / Mol. Gen. Genet. / Cloning and characterization of EcoRI and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6 by Timmis K. (1978)
Wise , E. M. , Jr. , and M. M. Abou-Donia . 1975 . Sulfonamide resistance mechanism in Esche)-ichia coli: Rplasmids can determine sulfonamide-resistant dihydropteroate synthases . Proc. Natl. Acad. Sci. U.S.A. 72 : 2621 - 2625 . (10.1073/pnas.72.7.2621) / Proc. Natl. Acad. Sci. U.S.A. / Sulfonamide resistance mechanism in Esche)-ichia coli: Rplasmids can determine sulfonamide-resistant dihydropteroate synthases by Wise E. M. (1975)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 12:01 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:37 p.m.)
Indexed	1 week, 6 days ago (Aug. 21, 2025, 1:41 p.m.)
Issued	45 years, 5 months ago (April 1, 1980)
Published	45 years, 5 months ago (April 1, 1980)
Published Print	45 years, 5 months ago (April 1, 1980)

Funders 0

None

BibTeX

@article{Swedberg_1980, title={Characterization of different plasmid-borne dihydropteroate synthases mediating bacterial resistance to sulfonamides}, volume={142}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.142.1.1-7.1980}, DOI={10.1128/jb.142.1.1-7.1980}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Swedberg, G and Sköld, O}, year={1980}, month=apr, pages={1–7} }

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