DOI: 10.1128/jb.137.1.1-5.1979. Regulation of cell size in the yeast Saccharomyces cerevisiae

Regulation of cell size in the yeast Saccharomyces cerevisiae

10.1128/jb.137.1.1-5.1979

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

For cells of the yeast Saccharomyces cerevisiae, the size at initiation of budding is proportional to growth rate for rates from 0.33 to 0.23 h-1. At growth rates lower than 0.23 h-1, cells displayed a minimum cell size at bud initiation independent of growth rate. Regardless of growth rate, cells displayed an increase in volume each time budding was initiated. When abnormally small cells, produced by starvation for nitrogen, were placed in fresh medium containing nitrogen but with different carbon sources, they did not initiate budding until they had grown to the critical size characteristic of that medium. Moreover, when cells were shifted from a medium supporting a low growth rate and small size at bud initiation to a medium supporting a higher growth rate and larger size at bud initiation, there was a transient accumulation of cells within G1. These results suggest that yeast cells are able to initiate cell division at different cell sizes and that regulation of cell size occurs within G1.

Bibliography

Johnston, G. C., Ehrhardt, C. W., Lorincz, A., & Carter, B. L. (1979). Regulation of cell size in the yeast Saccharomyces cerevisiae. Journal of Bacteriology, 137(1), 1â5.

Authors 4

G C Johnston (first)
C W Ehrhardt (additional)
A Lorincz (additional)
B L Carter (additional)

References 16 Referenced 198

Adams , J. 1977 . The interrelationship of cell growth and division in haploid and diploid cells of Saccharomyces cerevisiae . Exp. Cell Res. 106 : 267 - 275 . (10.1016/0014-4827(77)90172-0) / Exp. Cell Res. / The interrelationship of cell growth and division in haploid and diploid cells of Saccharomyces cerevisiae by Adams J. (1977)
la. Carter , B. L A. , A. Lorincz , and G. C. Johnston . 1978 . Protein synthesis, cell division and the cell cycle in Saccharomyces cerevisiae following a shift to a richer medium . J. Gen. Microbiol. 106 : 222 - 225 . / J. Gen. Microbiol. / Protein synthesis, cell division and the cell cycle in Saccharomyces cerevisiae following a shift to a richer medium by Carter B. L A. (1978)
Fantes , P. , and P. Nurse . 1977 . Control of cell size at division in fission yeast by a growth-modulated size control over nuclear division . Exp. Cell Res. 107 : 377 - 386 . (10.1016/0014-4827(77)90359-7) / Exp. Cell Res. / Control of cell size at division in fission yeast by a growth-modulated size control over nuclear division by Fantes P. (1977)
Fantes , P. A. , W. D. Grant , R. H. Pritchard , P. E. Sudbury , and A. E. Wheals . 1975 . The regulation of cell size and control of mitosis . J. Theor. Biol. 50 : 213 - 244 . (10.1016/0022-5193(75)90034-X) / J. Theor. Biol. / The regulation of cell size and control of mitosis by Fantes P. A. (1975)
Hartwell , L. H. 1970 . Periodic density fluctuation during the yeast cell cycle and the selection of synchronous cultures . J. Bacteriol. 104 : 1280 - 1285 . (10.1128/jb.104.3.1280-1285.1970) / J. Bacteriol. / Periodic density fluctuation during the yeast cell cycle and the selection of synchronous cultures by Hartwell L. H. (1970)
Hartwell , L. H. , and M. W. Unger . 1977 . Unequal division in Saccharomyces cerevisiae and its implications for the control of cell division . J. Cell Biol. 75 : 422 - 435 . (10.1083/jcb.75.2.422) / J. Cell Biol. / Unequal division in Saccharomyces cerevisiae and its implications for the control of cell division by Hartwell L. H. (1977)
Hayashibe , M. , and S. Katohda 1973 . Initiation of budding and chitin ring . J. Gen. Appl. Microbiol. 19 : 23 - 39 . (10.2323/jgam.19.23) / J. Gen. Appl. Microbiol. / Initiation of budding and chitin ring by Hayashibe M. (1973)
Jagadish , M. N. , and B. L. A. Carter . 1977 . Genetic control of cell division in yeast cultured at different growth rates . Nature (London) 269 : 145 - 147 . (10.1038/269145a0) / Nature (London) / Genetic control of cell division in yeast cultured at different growth rates by Jagadish M. N. (1977)
Jagadish , M. N. , A. Lorincz , and B. L. A. Carter . 1977 . Cell size and cell division in yeast cultured at different growth rates . FEMS Lett. 2 : 235 - 237 . (10.1111/j.1574-6968.1977.tb00949.x) / FEMS Lett. / Cell size and cell division in yeast cultured at different growth rates by Jagadish M. N. (1977)
Johnston , G. C. 1977 . Cell size and budding during starvation of the yeast Saccharomyces cerevisiae . J. Bacteriol. 132 : 738 - 739 . (10.1128/jb.132.2.738-739.1977) / J. Bacteriol. / Cell size and budding during starvation of the yeast Saccharomyces cerevisiae by Johnston G. C. (1977)
Johnston , G. C. , J. R. Pringle , and L. H. Hartwell . 1977 . Coordination of growth with cell division in the yeast Saccharomyces cerevisiae . Exp. Cell Res. 105 : 79 - 98 . (10.1016/0014-4827(77)90154-9) / Exp. Cell Res. / Coordination of growth with cell division in the yeast Saccharomyces cerevisiae by Johnston G. C. (1977)
Johnston , G. C. , R. A. Singer , and E. S. McFarlane . 1977 . Growth and cell division during nitrogen starvation of the yeast Saccharomyces cerevisiae . J. Bacteriol. 132 : 723 - 730 . (10.1128/jb.132.2.723-730.1977) / J. Bacteriol. / Growth and cell division during nitrogen starvation of the yeast Saccharomyces cerevisiae by Johnston G. C. (1977)
Lieblova , J. , K. Beran , and E. Streiblova . 1964 . Fraction of a population of Saccharomyces cerevisiae yeast by centrifugation in a dextran gradient . Folia Microbiol. (Prague) 9 : 205 - 213 . (10.1007/BF02875838) / Folia Microbiol. (Prague) / Fraction of a population of Saccharomyces cerevisiae yeast by centrifugation in a dextran gradient by Lieblova J. (1964)
McMurrough , T. , and A. H. Rose . 1967 . Effect of growth rate and substrate limitation on the composition and structure of the cell wall of Saccharomyces cerevisiae . Biochem. J. 105 : 189 - 203 . (10.1042/bj1050189) / Biochem. J. / Effect of growth rate and substrate limitation on the composition and structure of the cell wall of Saccharomyces cerevisiae by McMurrough T. (1967)
Mortimer , R. K. , and J. R. Johnson . 1959 . Life span of individual yeast cells . Nature (London) 183 : 1751 - 1752 . (10.1038/1831751a0) / Nature (London) / Life span of individual yeast cells by Mortimer R. K. (1959)
Slater , M. L , S. 0. Sharrow , and J. J. Gart . 1977 . Cell cycle of Saccharomyces cerevisiae in populations growing at different rates . Proc. Natl. Acad. Sci. U.S.A. 74 : 3850 - 3854 . (10.1073/pnas.74.9.3850) / Proc. Natl. Acad. Sci. U.S.A. / Cell cycle of Saccharomyces cerevisiae in populations growing at different rates by Slater M. L (1977)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 6:01 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 9:33 a.m.)
Indexed	6 days, 1 hour ago (Aug. 26, 2025, 3:11 a.m.)
Issued	46 years, 8 months ago (Jan. 1, 1979)
Published	46 years, 8 months ago (Jan. 1, 1979)
Published Print	46 years, 8 months ago (Jan. 1, 1979)

Funders 0

None

BibTeX

@article{Johnston_1979, title={Regulation of cell size in the yeast Saccharomyces cerevisiae}, volume={137}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.137.1.1-5.1979}, DOI={10.1128/jb.137.1.1-5.1979}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Johnston, G C and Ehrhardt, C W and Lorincz, A and Carter, B L}, year={1979}, month=jan, pages={1–5} }

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