DOI: 10.1128/jb.133.3.1419-1426.1978. Physiological characterization of an Escherichia coli mutant altered in the structure of murein lipoprotein

Physiological characterization of an Escherichia coli mutant altered in the structure of murein lipoprotein

10.1128/jb.133.3.1419-1426.1978

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Studies using isogenic transductant strains mlpA+ and mlpA as well as reversion analysis suggested that the physiological consequences of a structural gene mutation in murein lipoprotein include (i) increased sensitivity toward chelating agents ethylenediaminetetraacetic acid and ethyleneglycol-bis (beta-aminoethyl ether)-N,N-tetraacetic acid, (ii) leakage of periplasmic enzyme ribonuclease, (iii) weakened association between the outer membrane and the rigid layer accentuated by Mg2+ starvation, resulting in the formation of outer membrane blebs, and (iv) decreased growth rate in media of low ionic strength or low osmolarity. It is suggested that the bound form of lipoprotein plays an important role in the maintenance of the structural integrity of the outer membrane of the Escherichia coli cell envelope. Other outer membrane components may also contribute to the anchorage of outer membrane to the rigid layer, probably through ionic interactions with divalent cations. Using the phenotype of ribonuclease leakage as an unselected marker in a three-factor cross with P1 transduction, we were able to establish the gene order of man mlpA aroD pps on the E. coli chromosome.

Bibliography

Yem, D. W., & Wu, H. C. (1978). Physiological characterization of an Escherichia coli mutant altered in the structure of murein lipoprotein. Journal of Bacteriology, 133(3), 1419â1426.

Authors 2

D W Yem (first)
H C Wu (additional)

References 17 Referenced 137

Bachmann , B. J. , K. B. Low , and A. L. Taylor . 1976 . Recalibrated Linkage map of Escherichia coli K-12 . Bacteriol. Rev. 40 : 116 - 167 . (10.1128/br.40.1.116-167.1976) / Bacteriol. Rev. / Recalibrated Linkage map of Escherichia coli K-12 by Bachmann B. J. (1976)
Braun , V. 1973 . Molecular organization of the rigid layer and the cell wall of Escherichia coli . J. Infect. Dis. 128(Suppl. ): S9 - S16 . (10.1093/infdis/128.Supplement_1.S9) / J. Infect. Dis. / Molecular organization of the rigid layer and the cell wall of Escherichia coli by Braun V. (1973)
Braun , V. , and K. Rehn . 1969 . Chemical characterization, spatial distribution and function of a lipoprotein (murein-lipoprotein) of the E. coli cell wall. The specific effect of trypsin on the membrane structure . Eur. J. Biochem. 10 : 426 - 438 . (10.1111/j.1432-1033.1969.tb00707.x) / Eur. J. Biochem. / Chemical characterization, spatial distribution and function of a lipoprotein (murein-lipoprotein) of the E. coli cell wall. The specific effect of trypsin on the membrane structure by Braun V. (1969)
Burdett , I. D. J. , and R. G. E. Murray . 1974 . Electron microscope study of septum formation in Escherichia coli strains B and B/r during synchronous growth . J. Bacteriol. 119 : 1039 - 1056 . (10.1128/jb.119.3.1039-1056.1974) / J. Bacteriol. / Electron microscope study of septum formation in Escherichia coli strains B and B/r during synchronous growth by Burdett I. D. J. (1974)
Hirota , Y. , H. Suzuki , Y. Nishimura , and S. Yasuda . 1977 . On the process of cellular division in Escherichia coli: a mutant of E. coli lacking a murein lipoprotein . Proc. Natl. Acad. Sci. U.S.A. 74 : 1417 - 1420 . (10.1073/pnas.74.4.1417) / Proc. Natl. Acad. Sci. U.S.A. / On the process of cellular division in Escherichia coli: a mutant of E. coli lacking a murein lipoprotein by Hirota Y. (1977)
Inouye , M. 1974 . A three-dimensional molecular assembly model of a lipoprotein from the Escherichia coli outer membrane . Proc. Natl. Acad. Sci. U.S.A. 71 : 2396 - 2400 . (10.1073/pnas.71.6.2396) / Proc. Natl. Acad. Sci. U.S.A. / A three-dimensional molecular assembly model of a lipoprotein from the Escherichia coli outer membrane by Inouye M. (1974)
Inouye , S. , N. Lee , M. Inouye , H. C. Wu , Suzuki, Y. Nishimura , H. Iketani , and Y. Hirota . 1977 . Amino acid replacement in a mutant lipoprotein of the Escherichia coli outer membrane . J. Bacteriol. 132 : 308 - 313 . (10.1128/JB.132.1.308-313.1977) / J. Bacteriol. / Amino acid replacement in a mutant lipoprotein of the Escherichia coli outer membrane by Inouye S. (1977)
Lopes , J. , S. Gottfried , and L. I. Rothfield . 1972 . Leakage of periplasmic enzyme by mutants of Escherichia coli and Salmonella typhimurium: isolation of "periplasmic leaky " J. Bacteriol. 109 : 520 - 525 . (10.1128/JB.109.2.520-525.1972) / J. Bacteriol. / Leakage of periplasmic enzyme by mutants of Escherichia coli and Salmonella typhimurium: isolation of "periplasmic leaky by Lopes J. (1972)
Swank , R. T. , and K. D. Munkres . 1971 . Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate . Anal. Biochem. 39 : 462 - 477 . (10.1016/0003-2697(71)90436-2) / Anal. Biochem. / Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate by Swank R. T. (1971)
Torti , S. V. , and J. T. Park . 1976 . Lipoprotein of gramnegative bacteria is essential for growth and division . Nature (London) 263 : 323 - 326 . (10.1038/263323a0) / Nature (London) / Lipoprotein of gramnegative bacteria is essential for growth and division by Torti S. V. (1976)
Venkateswaran , P. S. , and H. C. Wu. 1972 . Isolation and characterization of a phosphonomycin-resistant mutant of Escherichia coli K-12 . J. Bacteriol. 110 : 935 - 944 . (10.1128/jb.110.3.935-944.1972) / J. Bacteriol. / Isolation and characterization of a phosphonomycin-resistant mutant of Escherichia coli K-12 by Venkateswaran P. S. (1972)
Weigand , R. A. , J. M. Shively , and J. W. Greenawalt . 1970 . Formation and structure of extra membranes in Escherichia coli . J. Bacteriol. 102 : 240 - 249 . (10.1128/jb.102.1.240-249.1970) / J. Bacteriol. / Formation and structure of extra membranes in Escherichia coli by Weigand R. A. (1970)
Weigand , R. A. , K. D. Vinci , and L. I. Rothfield . 1976 . Morphogenesis of the bacterial division septum: a new class of septation-defective mutants . Proc. Natl. Acad. Sci. U.S.A. 73 : 1882 - 1886 . (10.1073/pnas.73.6.1882) / Proc. Natl. Acad. Sci. U.S.A. / Morphogenesis of the bacterial division septum: a new class of septation-defective mutants by Weigand R. A. (1976)
Wu , H. C. , C. Hou , J. J. C. Lin , and D. W. Yem . 1977 . Biochemical characterization of a mutant lipoprotein of Escherichia coli . Proc. Natl. Acad. Sci. U.S.A. 74 : 1388 - 1392 . (10.1073/pnas.74.4.1388) / Proc. Natl. Acad. Sci. U.S.A. / Biochemical characterization of a mutant lipoprotein of Escherichia coli by Wu H. C. (1977)
Wu , H. C. , and J. J. C. Lin . 1976 . Escherichia coli mutants altered in murein lipoprotein . J. Bacteriol. 126 : 147 - 156 . (10.1128/JB.126.1.147-156.1976) / J. Bacteriol. / Escherichia coli mutants altered in murein lipoprotein by Wu H. C. (1976)
Wu , H. C. , and T. C. Wu. 1971 . Isolation and characterization of a glucosamine-requiring mutant of Escherichia coli K-12 defective in glucosamine-6-phosphate synthetase . J. Bacteriol. 105 : 455 - 466 . (10.1128/JB.105.2.455-466.1971) / J. Bacteriol. / Isolation and characterization of a glucosamine-requiring mutant of Escherichia coli K-12 defective in glucosamine-6-phosphate synthetase by Wu H. C. (1971)
Yem , D. W. , and H. C. Wu. 1977 . Genetic characterization of an Escherichia coli mutant altered in the structure of murein lipoprotein . J. Bacteriol. 131 : 759 - 764 . (10.1128/JB.131.3.759-764.1977) / J. Bacteriol. / Genetic characterization of an Escherichia coli mutant altered in the structure of murein lipoprotein by Yem D. W. (1977)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 11 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:28 p.m.)
Indexed	5 months ago (March 30, 2025, 3:05 p.m.)
Issued	47 years, 6 months ago (March 1, 1978)
Published	47 years, 6 months ago (March 1, 1978)
Published Print	47 years, 6 months ago (March 1, 1978)

Funders 0

None

BibTeX

@article{Yem_1978, title={Physiological characterization of an Escherichia coli mutant altered in the structure of murein lipoprotein}, volume={133}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.133.3.1419-1426.1978}, DOI={10.1128/jb.133.3.1419-1426.1978}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Yem, D W and Wu, H C}, year={1978}, month=mar, pages={1419–1426} }

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