Proteinase activities of Saccharomyces cerevisiae during sporulation
10.1128/jb.124.2.863-869.1975
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Sporulation in Saccharomyces cerevisiae occurs in the absence of a exogenous nitrogen source. Thus, the internal amino acid pool and the supply of nitrogen compounds from protein and nucleic acid turnover must be sufficient for new protein synthesis. Since sporulation involves an increased rate of protein turnover, an investigation was conducted of the changes in the specific activity of various proteinases. A minimum of 30% of the vegetative proteins was turned over during the course of sporulation. There was a 10- to 25-fold increase in specific activity of various proteinases, with a maximum activity around 20 h after transfer into the sporulation medium. The increase in activities was due to de novo synthesis since inhibition of protein synthesis by cycloheximide blocks both an increase in proteinase activities and sporulation. There was no increase observed in proteinase activities of nonsporogenic cultures (a and alpha/alpha strains) inoculated into the sporulation medium, suggesting that the increase in proteinase activities is "sporulation specific" and not a consequence of step-down conditions. The elution patterns through diethylaminoethyl-Sephadex chromatography of various proteinases extracted from T0 and T18 cells were similar, and no new species was observed.

Bibliography

Klar, A. J., & Halvorson, H. O. (1975). Proteinase activities of Saccharomyces cerevisiae during sporulation. Journal of Bacteriology, 124(2), 863–869.

Authors 2
  1. A J Klar (first)
  2. H O Halvorson (additional)
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Dates
Type When
Created 5 years, 7 months ago (Jan. 3, 2020, 10:47 a.m.)
Deposited 4 years ago (July 29, 2021, 1:19 p.m.)
Indexed 1 year, 8 months ago (Dec. 12, 2023, 7:56 p.m.)
Issued 49 years, 9 months ago (Nov. 1, 1975)
Published 49 years, 9 months ago (Nov. 1, 1975)
Published Print 49 years, 9 months ago (Nov. 1, 1975)
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@article{Klar_1975, title={Proteinase activities of Saccharomyces cerevisiae during sporulation}, volume={124}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.124.2.863-869.1975}, DOI={10.1128/jb.124.2.863-869.1975}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Klar, A J and Halvorson, H O}, year={1975}, month=nov, pages={863–869} }