DOI: 10.1128/jb.123.1.69-74.1975. Regulation of the Escherichia coli methylgalactoside transport system by gene mglD

Regulation of the Escherichia coli methylgalactoside transport system by gene mglD

10.1128/jb.123.1.69-74.1975

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Constitutive activity of the methylgalactoside transport system of Escherichia coli K-12 is shown to result from mutation of a genetic locus distinct from the two previously described regulatory loci for this permease. Employing an autoradiographic procedure whereby constitutive and inducible cells can be differentiated, it is demonstrated that this locus, termed mglD, is 20% cotransducible with ptsF by bacteriophage P1. Selection for constitutive mutants among an inducible population yielded cells who mutations mapped in mglD. Cotransduction of mglD with mglB, minus C, and minus A, three genes required for activity of the methylgalactoside transport system, is 95, 88, and 81%, respectively. The results of recombination studies employing three and four factors indicate that the order of genes in this region is ptsF, mglD, B, C, A.

Bibliography

Robbins, A. R. (1975). Regulation of the Escherichia coli methylgalactoside transport system by gene mglD. Journal of Bacteriology, 123(1), 69â74.

Authors 1

A R Robbins (first)

References 13 Referenced 23

Adelberg , E. A. , M. Mandel , and G. C. C. Chen . 1965 . Optimal conditions for mutagenesis by N'-methyl-N'-nitro-N-nitrosoguanidine in E. coli K12 . Biochem. Biophys. Res. Commun. 18 : 788 - 795 . (10.1016/0006-291X(65)90855-7) / Biochem. Biophys. Res. Commun. / Optimal conditions for mutagenesis by N'-methyl-N'-nitro-N-nitrosoguanidine in E. coli K12 by Adelberg E. A. (1965)
Ferenci , T. , and H. L. Kornberg . 1971 . Role of fructose-1,6-diphosphatase in fructose utilization by Escherichia coli . FEBS Lett. 14 : 360 - 363 . (10.1016/0014-5793(71)80301-0) / FEBS Lett. / Role of fructose-1,6-diphosphatase in fructose utilization by Escherichia coli by Ferenci T. (1971)
Ganesan , A. K. , and B. Rotman . 1965 . Transport systems for galactose and galactosides in Escherichia coli. I. Genetic determination and regulation of the methylgalactoside permease . J. Mol. Biol. 16 : 42 - 50 . (10.1016/S0022-2836(66)80261-9) / J. Mol. Biol. / Transport systems for galactose and galactosides in Escherichia coli. I. Genetic determination and regulation of the methylgalactoside permease by Ganesan A. K. (1965)
Lengeler , J. , K. 0. Hermann , H. J. Unsold , and W. Boos . 1971 . The regulation of the ,8-methylgalactoside transport system and of the galactose binding protein of Escherichia coli K12 . Eur. J. Biochem. 19 : 457 - 470 . (10.1111/j.1432-1033.1971.tb01336.x) / Eur. J. Biochem. / The regulation of the ,8-methylgalactoside transport system and of the galactose binding protein of Escherichia coli K12 by Lengeler J. (1971)
Lennox , E. S. 1955 . Transduction of linked genetic characters of the host by bacteriophage P1 . Virology 1 : 190 - 206 . (10.1016/0042-6822(55)90016-7) / Virology / Transduction of linked genetic characters of the host by bacteriophage P1 by Lennox E. S. (1955)
Malamy , M. H. 1966 . Frameshift mutations in the lactose operon of E. coli . Cold Spring Harbor Symp. Quant. Biol. 31 : 189 - 201 . (10.1101/SQB.1966.031.01.027) / Cold Spring Harbor Symp. Quant. Biol. / Frameshift mutations in the lactose operon of E. coli by Malamy M. H. (1966)
Ordal , G. W. , and J. Adler . 1974 . Isolation and complementation of mutants in galactose taxis and transport . J. Bacteriol. 117 : 509 - 516 . (10.1128/jb.117.2.509-516.1974) / J. Bacteriol. / Isolation and complementation of mutants in galactose taxis and transport by Ordal G. W. (1974)
Ordal , G. W. , and J. Adler . 1974 . Properties of mutants in galactose taxis and transport . J. Bacteriol. 117 : 517 - 526 . (10.1128/jb.117.2.517-526.1974) / J. Bacteriol. / Properties of mutants in galactose taxis and transport by Ordal G. W. (1974)
Robbins , A. R. , and B. Rotman . 1975 . Evidence for binding protein independent substrate translocation by the methylgalactoside transport system of Escherichia coli K12 . Proc. Natl. Acad. Sci. U.S.A. 72 : 423 - 427 . (10.1073/pnas.72.2.423) / Proc. Natl. Acad. Sci. U.S.A. / Evidence for binding protein independent substrate translocation by the methylgalactoside transport system of Escherichia coli K12 by Robbins A. R. (1975)
Rotman , B. , A. K. Ganesan , and R. Guzman . 1968 . Transport systems for galactose and galactosides in Escherichia coli. II. Substrate and inducer specificities . J. Mol. Biol. 36 : 247 - 260 . (10.1016/0022-2836(68)90379-3) / J. Mol. Biol. / Transport systems for galactose and galactosides in Escherichia coli. II. Substrate and inducer specificities by Rotman B. (1968)
Swanstrom , M. , and M. H. Adams . 1951 . Agar layer method for production of high titer phage stocks . Proc. Soc. Exp. Biol. Med. 78 : 372 - 375 . (10.3181/00379727-78-19076) / Proc. Soc. Exp. Biol. Med. / Agar layer method for production of high titer phage stocks by Swanstrom M. (1951)
Taylor , A. L. , and C. D. Trotter . 1972 . Linkage map of Escherichia coli strain K-12 . Bacteriol. Rev. 36 : 502 - 524 . (10.1128/br.36.4.504-524.1972) / Bacteriol. Rev. / Linkage map of Escherichia coli strain K-12 by Taylor A. L. (1972)
Wilson , T. H. , and E. R. Kashket . 1969 . Isolation and properties of thiogalactoside transacetylase-negative mutants of Escherichia coli . Biochim. Biophys. Acta 173 : 501 - 508 . (10.1016/0005-2736(69)90014-5) / Biochim. Biophys. Acta / Isolation and properties of thiogalactoside transacetylase-negative mutants of Escherichia coli by Wilson T. H. (1969)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 10:46 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:17 p.m.)
Indexed	1 year, 9 months ago (Nov. 12, 2023, 8:30 a.m.)
Issued	50 years, 2 months ago (July 1, 1975)
Published	50 years, 2 months ago (July 1, 1975)
Published Print	50 years, 2 months ago (July 1, 1975)

Funders 0

None

BibTeX

@article{Robbins_1975, title={Regulation of the Escherichia coli methylgalactoside transport system by gene mglD}, volume={123}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.123.1.69-74.1975}, DOI={10.1128/jb.123.1.69-74.1975}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Robbins, A R}, year={1975}, month=jul, pages={69–74} }

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