DOI: 10.1128/jb.119.3.952-960.1974. Protein Synthesis in Relation to Sporulation and Meiosis in Yeast

Protein Synthesis in Relation to Sporulation and Meiosis in Yeast

10.1128/jb.119.3.952-960.1974

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The dependence upon protein synthesis of physiological and biochemical events occurring during yeast sporulation was investigated. Protein synthesis was inhibited by cycloheximide. There was an early, irreversible sensitivity to inhibition with respect to cell viability and ascus formation; inhibition was reversible only if the cells were inhibited after, but not prior to, 2 to 3 h in sporulation medium. Interruption of protein synthesis of any time during sporulation inhibited all measurable metabolic and sporulation-specific processes except protein breakdown and, to some extent, ribonucleic acid synthesis. The time interval between the occurrence of an event and the protein synthesis necessary for that event was determined to be 2 to 3 h for ascus formation, ≤30 min for deoxyribonucleic acid synthesis, 1 h for tetranucleate cell formation, ≤30 min for ribonucleic acid breakdown, 1 to 2 h for glycogen synthesis, and 2 to 3 h for glycogen breakdown.

Bibliography

Magee, P. T., & Hopper, A. K. (1974). Protein Synthesis in Relation to Sporulation and Meiosis in Yeast. Journal of Bacteriology, 119(3), 952â960.

Authors 2

P. T. Magee (first)
Anita K. Hopper (additional)

References 15 Referenced 33

Croes , A. F. 1967 . Induction of meiosis in yeast. I. Timing of cytological and biochemical events . Planta 76 : 209 - 226 . / Planta / Induction of meiosis in yeast. I. Timing of cytological and biochemical events by Croes A. F. (1967)
Dickinson , H. G. , and J. Heslop-Harrison . 1970 . The ribosome cycle, nuclei, and cytoplasmic nucleoids in the meiocytes of Lillium . Protoplasma 69 : 187 - 200 . (10.1007/BF01280721) / Protoplasma / The ribosome cycle, nuclei, and cytoplasmic nucleoids in the meiocytes of Lillium by Dickinson H. G. (1970)
Esposito , M. S. , and R. E. Esposito . 1969 . The genetic control of sporulation in Saccharomyces. I. The isolation of temperature-sensitive sporulation-deficient mutants . Genetics 61 : 79 - 89 . (10.1093/genetics/61.1.79) / Genetics / The genetic control of sporulation in Saccharomyces. I. The isolation of temperature-sensitive sporulation-deficient mutants by Esposito M. S. (1969)
Esposito , M. S. , R. E. Esposito , M. Arnaud , and H. 0. Halvorson . 1969 . Acetate utilization and macromolecular synthesis during sporulation of yeast . J. Bacteriol. 100 : 180 - 186 . (10.1128/JB.100.1.180-186.1969) / J. Bacteriol. / Acetate utilization and macromolecular synthesis during sporulation of yeast by Esposito M. S. (1969)
Hartwell , L. H. , and C. S. McLaughlin . 1968 . A mutant of yeast apparently defective in the initiation of protein synthesis . Proc. Nat. Acad. Sci. U.S.A. 42 : 468 - 474 . / Proc. Nat. Acad. Sci. U.S.A. / A mutant of yeast apparently defective in the initiation of protein synthesis by Hartwell L. H. (1968)
Hereford , L. M. , and L. H. Hartwell . 1973 . Role of protein synthesis in the replication of yeast DNA . Nature N. Biol. 244 : 129 - 131 . (10.1038/newbio244129a0) / Nature N. Biol. / Role of protein synthesis in the replication of yeast DNA by Hereford L. M. (1973)
Hopper , A. K. , P. T. Magee , S. K. Welch , M. Friedman , and B. D. Hall . 1974 . Macromolecule synthesis and breakdown in relation to sporulation and meiosis in yeast . J. Bacteriol. 119 : 619 - 628 . (10.1128/jb.119.2.619-628.1974) / J. Bacteriol. / Macromolecule synthesis and breakdown in relation to sporulation and meiosis in yeast by Hopper A. K. (1974)
Hotta , Y. , M. Ito , and H. Stern . 1966 . Synthesis of DNA during meiosis . Proc. Nat. Acad. Sci. U.S.A. 56 : 1184 - 1191 . (10.1073/pnas.56.4.1184) / Proc. Nat. Acad. Sci. U.S.A. / Synthesis of DNA during meiosis by Hotta Y. (1966)
Mills , D. 1972 . Effect of pH on adenine and amino acid uptake during sporulation in Saccharomyces cerevisiae . J. Bacteriol. 112 : 519 - 526 . (10.1128/JB.112.1.519-526.1972) / J. Bacteriol. / Effect of pH on adenine and amino acid uptake during sporulation in Saccharomyces cerevisiae by Mills D. (1972)
Parchman , L. G. , and H. Stern . 1969 . The inhibition of protein synthesis in meiotic cells and its effect on chromosome behavior . Chromosoma 26 : 298 - 311 . (10.1007/BF00326524) / Chromosoma / The inhibition of protein synthesis in meiotic cells and its effect on chromosome behavior by Parchman L. G. (1969)
Puglisi , P. P. , and E. Zennaro . 1971 . Erythromycin inhibition of sporulation in Saccharomyces cerevisiae . Experientia 27 : 963 - 964 . (10.1007/BF02135775) / Experientia / Erythromycin inhibition of sporulation in Saccharomyces cerevisiae by Puglisi P. P. (1971)
Roth , R. , and H. 0. Halvorson . 1969 . Sporulation of yeast harvested during logarithmic growth . J. Bacteriol. 98 : 831 - 832 . (10.1128/JB.98.2.831-832.1969) / J. Bacteriol. / Sporulation of yeast harvested during logarithmic growth by Roth R. (1969)
Roth , R. M. , and C. Dampier . 1972 . Dependence of ribonucleic acid synthesis on continuous protein synthesis in yeast . J. Bacteriol. 109 : 773 - 779 . (10.1128/JB.109.2.773-779.1972) / J. Bacteriol. / Dependence of ribonucleic acid synthesis on continuous protein synthesis in yeast by Roth R. M. (1972)
Stern , H. , and Y. Hotta . 1969 . DNA synthesis in relation to chromosome pairing and chiasma formation . Genetics 61(Suppl. ): 29 - 39 . / Genetics / DNA synthesis in relation to chromosome pairing and chiasma formation by Stern H. (1969)
Williamson , D. H. 1973 . Replication of the nuclear genome in the yeast does not require concomitant protein synthesis . Biochem. Biophys. Res. Commun. 52 : 731 - 740 . (10.1016/0006-291X(73)90998-4) / Biochem. Biophys. Res. Commun. / Replication of the nuclear genome in the yeast does not require concomitant protein synthesis by Williamson D. H. (1973)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 9:57 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:13 p.m.)
Indexed	1 day, 19 hours ago (Sept. 4, 2025, 9:33 a.m.)
Issued	51 years ago (Sept. 1, 1974)
Published	51 years ago (Sept. 1, 1974)
Published Print	51 years ago (Sept. 1, 1974)

Funders 0

None

BibTeX

@article{Magee_1974, title={Protein Synthesis in Relation to Sporulation and Meiosis in Yeast}, volume={119}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.119.3.952-960.1974}, DOI={10.1128/jb.119.3.952-960.1974}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Magee, P. T. and Hopper, Anita K.}, year={1974}, month=sep, pages={952–960} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        9,
        4
      ]
    ],
    "date-time": "2025-09-04T13:33:14Z",
    "timestamp": 1756992794219
  },
  "reference-count": 15,
  "publisher": "American Society for Microbiology",
  "issue": "3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1974,
            9,
            1
          ]
        ],
        "date-time": "1974-09-01T00:00:00Z",
        "timestamp": 147225600000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1974,
        9
      ]
    ]
  },
  "abstract": "<jats:p>The dependence upon protein synthesis of physiological and biochemical events occurring during yeast sporulation was investigated. Protein synthesis was inhibited by cycloheximide. There was an early, irreversible sensitivity to inhibition with respect to cell viability and ascus formation; inhibition was reversible only if the cells were inhibited after, but not prior to, 2 to 3 h in sporulation medium. Interruption of protein synthesis of any time during sporulation inhibited all measurable metabolic and sporulation-specific processes except protein breakdown and, to some extent, ribonucleic acid synthesis. The time interval between the occurrence of an event and the protein synthesis necessary for that event was determined to be 2 to 3 h for ascus formation, \u226430 min for deoxyribonucleic acid synthesis, 1 h for tetranucleate cell formation, \u226430 min for ribonucleic acid breakdown, 1 to 2 h for glycogen synthesis, and 2 to 3 h for glycogen breakdown.</jats:p>",
  "DOI": "10.1128/jb.119.3.952-960.1974",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2020,
        1,
        3
      ]
    ],
    "date-time": "2020-01-03T14:57:41Z",
    "timestamp": 1578063461000
  },
  "page": "952-960",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 33,
  "title": "Protein Synthesis in Relation to Sporulation and Meiosis in Yeast",
  "prefix": "10.1128",
  "volume": "119",
  "author": [
    {
      "given": "P. T.",
      "family": "Magee",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Genetics University of Washington, Seattle, Washington 98195"
        }
      ]
    },
    {
      "given": "Anita K.",
      "family": "Hopper",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemistry, University of Washington, Seattle, Washington 98195"
        }
      ]
    }
  ],
  "member": "235",
  "reference": [
    {
      "key": "p_1",
      "first-page": "209",
      "article-title": "Induction of meiosis in yeast. I. Timing of cytological and biochemical events",
      "volume": "76",
      "author": "Croes A. F.",
      "year": "1967",
      "unstructured": "Croes , A. F. 1967 . Induction of meiosis in yeast. I. Timing of cytological and biochemical events . Planta 76 : 209 - 226 .",
      "journal-title": "Planta"
    },
    {
      "key": "p_2",
      "doi-asserted-by": "crossref",
      "first-page": "187",
      "DOI": "10.1007/BF01280721",
      "article-title": "The ribosome cycle, nuclei, and cytoplasmic nucleoids in the meiocytes of Lillium",
      "volume": "69",
      "author": "Dickinson H. G.",
      "year": "1970",
      "unstructured": "Dickinson , H. G. , and J. Heslop-Harrison . 1970 . The ribosome cycle, nuclei, and cytoplasmic nucleoids in the meiocytes of Lillium . Protoplasma 69 : 187 - 200 .",
      "journal-title": "Protoplasma"
    },
    {
      "key": "p_3",
      "doi-asserted-by": "crossref",
      "first-page": "79",
      "DOI": "10.1093/genetics/61.1.79",
      "article-title": "The genetic control of sporulation in Saccharomyces. I. The isolation of temperature-sensitive sporulation-deficient mutants",
      "volume": "61",
      "author": "Esposito M. S.",
      "year": "1969",
      "unstructured": "Esposito , M. S. , and R. E. Esposito . 1969 . The genetic control of sporulation in Saccharomyces. I. The isolation of temperature-sensitive sporulation-deficient mutants . Genetics 61 : 79 - 89 .",
      "journal-title": "Genetics"
    },
    {
      "key": "p_4",
      "doi-asserted-by": "crossref",
      "first-page": "180",
      "DOI": "10.1128/JB.100.1.180-186.1969",
      "article-title": "Acetate utilization and macromolecular synthesis during sporulation of yeast",
      "volume": "100",
      "author": "Esposito M. S.",
      "year": "1969",
      "unstructured": "Esposito , M. S. , R. E. Esposito , M. Arnaud , and H. 0. Halvorson . 1969 . Acetate utilization and macromolecular synthesis during sporulation of yeast . J. Bacteriol. 100 : 180 - 186 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_5",
      "first-page": "468",
      "article-title": "A mutant of yeast apparently defective in the initiation of protein synthesis",
      "volume": "42",
      "author": "Hartwell L. H.",
      "year": "1968",
      "unstructured": "Hartwell , L. H. , and C. S. McLaughlin . 1968 . A mutant of yeast apparently defective in the initiation of protein synthesis . Proc. Nat. Acad. Sci. U.S.A. 42 : 468 - 474 .",
      "journal-title": "Proc. Nat. Acad. Sci. U.S.A."
    },
    {
      "key": "p_6",
      "doi-asserted-by": "crossref",
      "first-page": "129",
      "DOI": "10.1038/newbio244129a0",
      "article-title": "Role of protein synthesis in the replication of yeast DNA",
      "volume": "244",
      "author": "Hereford L. M.",
      "year": "1973",
      "unstructured": "Hereford , L. M. , and L. H. Hartwell . 1973 . Role of protein synthesis in the replication of yeast DNA . Nature N. Biol. 244 : 129 - 131 .",
      "journal-title": "Nature N. Biol."
    },
    {
      "key": "p_7",
      "doi-asserted-by": "crossref",
      "first-page": "619",
      "DOI": "10.1128/jb.119.2.619-628.1974",
      "article-title": "Macromolecule synthesis and breakdown in relation to sporulation and meiosis in yeast",
      "volume": "119",
      "author": "Hopper A. K.",
      "year": "1974",
      "unstructured": "Hopper , A. K. , P. T. Magee , S. K. Welch , M. Friedman , and B. D. Hall . 1974 . Macromolecule synthesis and breakdown in relation to sporulation and meiosis in yeast . J. Bacteriol. 119 : 619 - 628 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_8",
      "doi-asserted-by": "crossref",
      "first-page": "1184",
      "DOI": "10.1073/pnas.56.4.1184",
      "article-title": "Synthesis of DNA during meiosis",
      "volume": "56",
      "author": "Hotta Y.",
      "year": "1966",
      "unstructured": "Hotta , Y. , M. Ito , and H. Stern . 1966 . Synthesis of DNA during meiosis . Proc. Nat. Acad. Sci. U.S.A. 56 : 1184 - 1191 .",
      "journal-title": "Proc. Nat. Acad. Sci. U.S.A."
    },
    {
      "key": "p_9",
      "doi-asserted-by": "crossref",
      "first-page": "519",
      "DOI": "10.1128/JB.112.1.519-526.1972",
      "article-title": "Effect of pH on adenine and amino acid uptake during sporulation in Saccharomyces cerevisiae",
      "volume": "112",
      "author": "Mills D.",
      "year": "1972",
      "unstructured": "Mills , D. 1972 . Effect of pH on adenine and amino acid uptake during sporulation in Saccharomyces cerevisiae . J. Bacteriol. 112 : 519 - 526 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_10",
      "doi-asserted-by": "crossref",
      "first-page": "298",
      "DOI": "10.1007/BF00326524",
      "article-title": "The inhibition of protein synthesis in meiotic cells and its effect on chromosome behavior",
      "volume": "26",
      "author": "Parchman L. G.",
      "year": "1969",
      "unstructured": "Parchman , L. G. , and H. Stern . 1969 . The inhibition of protein synthesis in meiotic cells and its effect on chromosome behavior . Chromosoma 26 : 298 - 311 .",
      "journal-title": "Chromosoma"
    },
    {
      "key": "p_11",
      "doi-asserted-by": "crossref",
      "first-page": "963",
      "DOI": "10.1007/BF02135775",
      "article-title": "Erythromycin inhibition of sporulation in Saccharomyces cerevisiae",
      "volume": "27",
      "author": "Puglisi P. P.",
      "year": "1971",
      "unstructured": "Puglisi , P. P. , and E. Zennaro . 1971 . Erythromycin inhibition of sporulation in Saccharomyces cerevisiae . Experientia 27 : 963 - 964 .",
      "journal-title": "Experientia"
    },
    {
      "key": "p_12",
      "doi-asserted-by": "crossref",
      "first-page": "831",
      "DOI": "10.1128/JB.98.2.831-832.1969",
      "article-title": "Sporulation of yeast harvested during logarithmic growth",
      "volume": "98",
      "author": "Roth R.",
      "year": "1969",
      "unstructured": "Roth , R. , and H. 0. Halvorson . 1969 . Sporulation of yeast harvested during logarithmic growth . J. Bacteriol. 98 : 831 - 832 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_13",
      "doi-asserted-by": "crossref",
      "first-page": "773",
      "DOI": "10.1128/JB.109.2.773-779.1972",
      "article-title": "Dependence of ribonucleic acid synthesis on continuous protein synthesis in yeast",
      "volume": "109",
      "author": "Roth R. M.",
      "year": "1972",
      "unstructured": "Roth , R. M. , and C. Dampier . 1972 . Dependence of ribonucleic acid synthesis on continuous protein synthesis in yeast . J. Bacteriol. 109 : 773 - 779 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_14",
      "first-page": "29",
      "article-title": "DNA synthesis in relation to chromosome pairing and chiasma formation",
      "volume": "61",
      "author": "Stern H.",
      "year": "1969",
      "unstructured": "Stern , H. , and Y. Hotta . 1969 . DNA synthesis in relation to chromosome pairing and chiasma formation . Genetics 61(Suppl. ): 29 - 39 .",
      "journal-title": "Genetics"
    },
    {
      "key": "p_15",
      "doi-asserted-by": "crossref",
      "first-page": "731",
      "DOI": "10.1016/0006-291X(73)90998-4",
      "article-title": "Replication of the nuclear genome in the yeast does not require concomitant protein synthesis",
      "volume": "52",
      "author": "Williamson D. H.",
      "year": "1973",
      "unstructured": "Williamson , D. H. 1973 . Replication of the nuclear genome in the yeast does not require concomitant protein synthesis . Biochem. Biophys. Res. Commun. 52 : 731 - 740 .",
      "journal-title": "Biochem. Biophys. Res. Commun."
    }
  ],
  "container-title": "Journal of Bacteriology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.119.3.952-960.1974",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.119.3.952-960.1974",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        7,
        29
      ]
    ],
    "date-time": "2021-07-29T17:13:21Z",
    "timestamp": 1627578801000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/jb.119.3.952-960.1974"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1974,
        9
      ]
    ]
  },
  "references-count": 15,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1974,
          9
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/jb.119.3.952-960.1974"
  ],
  "URL": "http://dx.doi.org/10.1128/jb.119.3.952-960.1974",
  "relation": {},
  "ISSN": [
    "0021-9193",
    "1098-5530"
  ],
  "subject": [],
  "container-title-short": "J Bacteriol",
  "published": {
    "date-parts": [
      [
        1974,
        9
      ]
    ]
  },
  "assertion": [
    {
      "value": "2021-02-02",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}