DOI: 10.1128/jb.119.3.736-747.1974. Culture Medium for Enterobacteria

10.1128/jb.119.3.736-747.1974

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

A new minimal medium for enterobacteria has been developed. It supports growth of Escherichia coli and Salmonella typhimurium at rates comparable to those of any of the traditional media that have high phosphate concentrations, but each of the macronutrients (phosphate, sulfate, and nitrogen) is present at a sufficiently low level to permit isotopic labeling. Buffering capacity is provided by an organic dipolar ion, morpholinopropane sulfonate, which has a desirable pK (7.2) and no apparent inhibitory effect on growth. The medium has been developed with the objectives of (i) providing reproducibility of chemical composition, (ii) meeting the experimentally determined nutritional needs of the cell, (iii) avoiding an unnecessary excess of the major ionic species, (iv) facilitating the adjustment of the levels of individual ionic species, both for isotopic labeling and for nutritional studies, (v) supplying a complete array of micronutrients, (vi) setting a particular ion as the crop-limiting factor when the carbon and energy source is in excess, and (vii) providing maximal convenience in the manufacture and storage of the medium.

Bibliography

Neidhardt, F. C., Bloch, P. L., & Smith, D. F. (1974). Culture Medium for Enterobacteria. Journal of Bacteriology, 119(3), 736â747.

Authors 3

Frederick C. Neidhardt (first)
Philip L. Bloch (additional)
David F. Smith (additional)

References 10 Referenced 2,550

Ajl , S. J. , and C. H. Werkman . 1948 . Replacement of CO2 in heterotrophic metabolism . Arch. Biochem. 19 : 483 - 492 . / Arch. Biochem. / Replacement of CO2 in heterotrophic metabolism by Ajl S. J. (1948)
Davis , B. D. , and E. S. Mingioli . 1950 . Mutants of Escherichia coli requiring methionine or vitamin B ,. J. Bacteriol. 60 : 17 - 28 . (10.1128/JB.60.1.17-28.1950) / J. Bacteriol. / Mutants of Escherichia coli requiring methionine or vitamin B by Davis B. D. (1950)
Eagle , H. 1971 . Buffer combinations for mammalian cell culture . Science 174 : 500 - 503 . (10.1126/science.174.4008.500) / Science / Buffer combinations for mammalian cell culture by Eagle H. (1971)
Fraenkel , D. G. , and F. C. Neidhardt . 1961 . Use of chloramphenicol to study control of RNA synthesis in bacteria . Biochim. Biophys. Acta 53 : 96 - 110 . (10.1016/0006-3002(61)90797-1) / Biochim. Biophys. Acta / Use of chloramphenicol to study control of RNA synthesis in bacteria by Fraenkel D. G. (1961)
Good N. E. and S. Izawa. 1972. Hydrogen ion buffers p. 53-68. In A. San Pietro (ed.) Methods in enzymology vol. 24 part B. Academic Press Inc. New York. (10.1016/0076-6879(72)24054-X)
Good , N. E. , G. D. Winget , W. Winter , T. N. Connolly , S. Izawa , and R. M. M. Singh . 1966 . Hydrogen ion buffers for biological research . Biochemistry 5 : 467 - 477 . (10.1021/bi00866a011) / Biochemistry / Hydrogen ion buffers for biological research by Good N. E. (1966)
Kaempfer , R. 0. R., and B. Magasanik . 1967 . Effect of infection with T-even phage on the inducible synthesis of beta-galactosidase in Escherichia coli . J. Mol. Biol. 27 : 453 - 468 . (10.1016/0022-2836(67)90051-4) / J. Mol. Biol. / Effect of infection with T-even phage on the inducible synthesis of beta-galactosidase in Escherichia coli by Kaempfer R. (1967)
Machlis , L. 1953 . Growth and nutrition of water molds in the subgenus Eulomyces. m. Carbon sources . Amer. J. Botany 40 : 460 - 464 . (10.1002/j.1537-2197.1953.tb06506.x) / Amer. J. Botany / Growth and nutrition of water molds in the subgenus Eulomyces. m. Carbon sources by Machlis L. (1953)
Miller J. H. 1972. Experiments in molecular genetics p. 431-432. Cold Spring Harbor Laboratory Cold Spring Harbor New York.
Vogel , H. J. , and D. M. Bonner . 1955 . Acetylornithinase of Escherichia coli: partial purification and some properties . J. Biol. Chem. 218 : 97 - 106 . (10.1016/S0021-9258(18)65874-0) / J. Biol. Chem. / Acetylornithinase of Escherichia coli: partial purification and some properties by Vogel H. J. (1955)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 10:02 a.m.)
Deposited	4 years ago (July 29, 2021, 1:13 p.m.)
Indexed	1 week ago (Aug. 20, 2025, 9:02 a.m.)
Issued	50 years, 11 months ago (Sept. 1, 1974)
Published	50 years, 11 months ago (Sept. 1, 1974)
Published Print	50 years, 11 months ago (Sept. 1, 1974)

Funders 0

None

BibTeX

@article{Neidhardt_1974, title={Culture Medium for Enterobacteria}, volume={119}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.119.3.736-747.1974}, DOI={10.1128/jb.119.3.736-747.1974}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Neidhardt, Frederick C. and Bloch, Philip L. and Smith, David F.}, year={1974}, month=sep, pages={736–747} }

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