Alterations in the Outer Membrane of the Cell Envelope of Heptose-Deficient Mutants of Escherichia coli
10.1128/jb.117.2.527-543.1974
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The composition of the cell envelope of a heptose-deficient lipopolysaccharide mutant of Escherichia coli , GR467, was studied after fractionation into its outer and cytoplasmic membrane components by means of sucrose density gradient centrifugation. The outer membrane of GR467 had a lower density than that of its parent strain, CR34. Analysis of the fractionated membranes of GR467 indicated that the phospholipid-to-protein ratio had increased 2.4-fold in the outer membrane. The ratio in the mutant cytoplasmic membrane was also increased, although to a lesser extent. By employing a third parameter, the lipid A content of the outer membrane, it was found that the observed phospholipid-to-protein change in the outer membrane was due predominantly to a decrease in the relative amount of protein. This decrease in protein was particularly significant, since it was concomitant with a 68% decrease in the lipid A recovered in the outer membrane of GR467 relative to the lipid A recovered in the outer membrane of CR34. Similar findings were observed in a second heptose-deficient mutant of E. coli , RC-59. The apparent protein deficiency in GR467 was further studied by subjecting solubilized envelope proteins to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It was found that major envelope proteins which were localized in the outer membrane were greatly diminished in GR467. Two revertants of GR467 with the wild-type amounts of heptose had wild-type relative levels of protein in their outer membranes. A partial heptose revertant had a relative level of protein in its outer membrane between those of the mutant and wild type.

Bibliography

Koplow, J., & Goldfine, H. (1974). Alterations in the Outer Membrane of the Cell Envelope of Heptose-Deficient Mutants of Escherichia coli. Journal of Bacteriology, 117(2), 527–543.

Authors 2 University of Pennsylvania
  1. Jane Koplow (first) University of Pennsylvania
  2. Howard Goldfine (additional) University of Pennsylvania
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Dates
Type When
Created 5 years, 8 months ago (Jan. 3, 2020, 9:59 a.m.)
Deposited 4 years, 3 months ago (May 27, 2021, 9:52 p.m.)
Indexed 4 months, 4 weeks ago (April 8, 2025, 10:21 p.m.)
Issued 51 years, 7 months ago (Feb. 1, 1974)
Published 51 years, 7 months ago (Feb. 1, 1974)
Published Print 51 years, 7 months ago (Feb. 1, 1974)
Funders 0

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@article{Koplow_1974, title={Alterations in the Outer Membrane of the Cell Envelope of Heptose-Deficient Mutants of Escherichia coli}, volume={117}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.117.2.527-543.1974}, DOI={10.1128/jb.117.2.527-543.1974}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Koplow, Jane and Goldfine, Howard}, year={1974}, month=feb, pages={527–543} }