Process of Cellular Division in Escherichia coli : Physiological Study on Thermosensitive Mutants Defective in Cell Division
10.1128/jb.116.1.314-322.1973
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Thermosensitive fts mutants of Escherichia coli belonging to seven previously identified genetic classes ( ftsA to ftsG ) were studied from a physiological standpoint. These mutants immediately stopped dividing and formed multinucleated filaments when the temperature was shifted to 41 C. Macromolecular syntheses (deoxyribonucleic acid), ribonucleic acid, cell mass, and murein) continued exponentially for at least 40 to 120 min. The number of surviving bacteria remained constant during the time of incubation, and this number began to decrease exponentially, as the rate of cell mass increase leveled off from the initial rate. The recovery of cell division at 30 C in these filamentous cells was studied after 60 min of incubation at 41 C. The existence of three types of mutants was shown. The ftsA and ftsE mutants resumed cell division without new protein synthesis; ftsD mutants resumed cell division only if new protein synthesis occured, while ftsB, C, F and G mutants did not resume cell division at all. No alteration in the cell envelope was detected by the method used here, although the ftsA, B, D, F and G mutations, in contrast with ftsC and E , caused an increased resistance to penicillin G. It was also shown that the recA mutation did not suppress the effect of the fts mutations and that none of the lysogenic fts mutants induced prophage multiplication while forming filaments. The effects of osmotic pressure and salts which rescue the mutant phenotype is described.

Bibliography

Ricard, M., & Hirota, Y. (1973). Process of Cellular Division in Escherichia coli  : Physiological Study on Thermosensitive Mutants Defective in Cell Division. Journal of Bacteriology, 116(1), 314–322.

Authors 2
  1. Matthieu Ricard (first)
  2. Yukinori Hirota (additional)
References 43 Referenced 109
  1. Allen , R. G. , J. A. Smith , R. C. Knudsen , and J. A. Walker . 1972 . Initial charactetization of temperaturesensitive cell division mutants of Escherichia coli . Biochem. Biophys. Res. Commun. 47 : 1074 - 1079 . (10.1016/0006-291X(72)90943-6) / Biochem. Biophys. Res. Commun. / Initial charactetization of temperaturesensitive cell division mutants of Escherichia coli by Allen R. G. (1972)
  2. Castellazzi , M. , J. George , and G. Buttin . 1972 . Prophage induction and cell division in E. coli. I. Further characterization of the thermosensitive mutation tif-1 whose expression mimics the effect of UV-irradiation . Mol. Gen. Genet. 119 : 139 - 152 . (10.1007/BF00269133) / Mol. Gen. Genet. / Prophage induction and cell division in E. coli. I. Further characterization of the thermosensitive mutation tif-1 whose expression mimics the effect of UV-irradiation by Castellazzi M. (1972)
  3. Castellazzi , M. , J. George , and G. Buttin . 1972 . Prophage induction and cell division in E. coli. II. Linked (recA, zab) and unlinked (lex) suppressors of tif-1 mediated induction and filamentation . Mol. Gen. Genet. 119 : 153 - 174 . (10.1007/BF00269134) / Mol. Gen. Genet. / Prophage induction and cell division in E. coli. II. Linked (recA, zab) and unlinked (lex) suppressors of tif-1 mediated induction and filamentation by Castellazzi M. (1972)
  4. Clark , J. 1968 . Regulation of deoxyribonucleic acid replication and cell division in Escherichia coli B/r . J. Bacteriol. 96 : 1214 - 1224 . (10.1128/jb.96.4.1214-1224.1968) / J. Bacteriol. / Regulation of deoxyribonucleic acid replication and cell division in Escherichia coli B/r by Clark J. (1968)
  5. Cooper , S. , and C. E. Helmstetter . 1968 . Chromosome replication and the division cycle of Escherichia coli B/r . J. Mol. Biol. 31 : 519 - 540 . (10.1016/0022-2836(68)90425-7) / J. Mol. Biol. / Chromosome replication and the division cycle of Escherichia coli B/r by Cooper S. (1968)
  6. Fisher , W. D. , H. I. Adler , F. W. Shull Jr ., and A. Cohen . 1969 . Properties of a cell fraction that repairs damage to the cell division mechanism of Escherichia coli . J. Bacteriol. 97 : 500 - 505 . (10.1128/jb.97.2.500-505.1969) / J. Bacteriol. / Properties of a cell fraction that repairs damage to the cell division mechanism of Escherichia coli by Fisher W. D. (1969)
  7. Green , M. H. L. , J. Greenberg , and J. Donch . 1970 . Effect of a recA gene on cell division and capsular polysaccharide production in a Ion strain of Escherichia coli . Genet. Res. Camb. 14 : 159 - 169 . (10.1017/S0016672300001993) / Genet. Res. Camb. / Effect of a recA gene on cell division and capsular polysaccharide production in a Ion strain of Escherichia coli by Green M. H. L. (1970)
  8. Hartmann , R. , J. V. Holtje , and U. Schwarz . 1972 . Targets of penicillin action in Escherichia coli . Nature (London) 235 : 426 - 429 . (10.1038/235426a0) / Nature (London) / Targets of penicillin action in Escherichia coli by Hartmann R. (1972)
  9. Hawthorne , D. C. , and J. Friis . 1964 . Osmotic remedial mutants. A new classification for nutritional mutants in yeast . Genetics 50 : 829 - 839 . (10.1093/genetics/50.5.829) / Genetics / Osmotic remedial mutants. A new classification for nutritional mutants in yeast by Hawthorne D. C. (1964)
  10. Helmstetter , C. E. , and S. Cooper . 1968 . DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r . J. Mol. Biol. 31 : 507 - 518 . (10.1016/0022-2836(68)90424-5) / J. Mol. Biol. / DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r by Helmstetter C. E. (1968)
  11. Helmstetter , C. E. , and 0. Pierucci . 1968 . Cell division during inhibition of deoxyribonucleic acid synthesis . J. Bacteriol. 95 : 1627 - 1639 . (10.1128/jb.95.5.1627-1633.1968) / J. Bacteriol. / Cell division during inhibition of deoxyribonucleic acid synthesis by Helmstetter C. E. (1968)
  12. Henning U. and U. Schwarz. 1973. Determinants of cell shape. In L. Leive (ed.) Bacterial surface. Dekker Publ. Co. New York.
  13. Hertman , I. , and S. E. Luria . 1967 . Transduction studies on the role of a rec + gene in the ultraviolet induction of prophage lambda . J. Mol. Biol. 23 : 117 - 133 . (10.1016/S0022-2836(67)80021-4) / J. Mol. Biol. / Transduction studies on the role of a rec + gene in the ultraviolet induction of prophage lambda by Hertman I. (1967)
  14. Hirota , Y. , F. Jacob , A. Ryter , G. Buttin , and T. Nakai . 1968 . On the process of cellular division in Escherichia coli. I. Asymmetrical cell division and production of deoxyribonucleic acid-less bacteria . J. Mol. Biol. 35 : 175 - 192 . / J. Mol. Biol. / On the process of cellular division in Escherichia coli. I. Asymmetrical cell division and production of deoxyribonucleic acid-less bacteria by Hirota Y. (1968)
  15. Hirota , Y. , J. Mordoh , and F. Jacob . 1970 . On the process of cellular division in Escherichia coli. III. Thermosensitive mutants of E. coli altered in the process of DNA initiation . J. Mol. Biol. 53 : 369 - 387 . / J. Mol. Biol. / On the process of cellular division in Escherichia coli. III. Thermosensitive mutants of E. coli altered in the process of DNA initiation by Hirota Y. (1970)
  16. Hirota Y. and M. Ricard. 1972. Production of DNA-less bacteria p. 29-50. In Biology and radiobiology of anucleate systems I. Academic Press Inc. New York. (10.1016/B978-0-12-115001-3.50008-6)
  17. Hirota Y. M. Ricard and B. Shapiro. 1971. The use of thermosensitive mutants of E. coli in the analysis of cell division p. 13-31. In L. A. Manson (ed.) Biomembranes vol. 2. Plenum Publ. Co. New York. (10.1007/978-1-4684-3330-2_5)
  18. Hirota , Y. , A. Ryter , and F. Jacob . 1968 . Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division . Cold Spring Harbor Symp. Quant. Biol. 33 : 677 - 693 . (10.1101/SQB.1968.033.01.077) / Cold Spring Harbor Symp. Quant. Biol. / Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division by Hirota Y. (1968)
  19. Hirota , Y. , S. Wakil , B. Shapiro , A. Ryter , J. Hurwitz , and F. Jacob . 1970 . Sur un mutant thermosensible d'Escherichia coli pr 6sentant des anomalies de la membrane . C. R. Acad. Sci. 269 : 1346 - 1348 . / C. R. Acad. Sci. / Sur un mutant thermosensible d'Escherichia coli pr 6sentant des anomalies de la membrane by Hirota Y. (1970)
  20. Howard-Flanders , P. , E. Simon , and L. Theriot . 1964 . A locus that controls filament formation and sensitivity to radiation in Escherichia coli K12 . Genetics 49 : 237 - 246 . (10.1093/genetics/49.2.237) / Genetics / A locus that controls filament formation and sensitivity to radiation in Escherichia coli K12 by Howard-Flanders P. (1964)
  21. Inouye , M. 1969 . Unlinking of cell division from deoxyribonucleic acid replication in a temperature-sensitive deoxyribonucleic acid synthesis mutant of Escherichia coli . J. Bacteriol. 99 : 842 - 850 . (10.1128/jb.99.3.842-850.1969) / J. Bacteriol. / Unlinking of cell division from deoxyribonucleic acid replication in a temperature-sensitive deoxyribonucleic acid synthesis mutant of Escherichia coli by Inouye M. (1969)
  22. Inouye , M. 1971 . Pleiotropic effect of the recA gene of Escherichia coli: uncoupling of cell division from DNA replication . J. Bacteriol. 106 : 531 - 542 . (10.1128/jb.106.2.539-542.1971) / J. Bacteriol. / Pleiotropic effect of the recA gene of Escherichia coli: uncoupling of cell division from DNA replication by Inouye M. (1971)
  23. Jacob , F. , S. Brenner , and F. Cuzin . 1963 . On the regulation of DNA replication in bacteria . Cold Spring Harbor Symp. Quant. Biol. 28 : 329 - 348 . (10.1101/SQB.1963.028.01.048) / Cold Spring Harbor Symp. Quant. Biol. / On the regulation of DNA replication in bacteria by Jacob F. (1963)
  24. Kaiser , A. D. , and F. Jacob . 1957 . Recombination between related temperate bacteriophages and the genetic control of immunity and prophage localization . Virology 4 : 509 - 521 . (10.1016/0042-6822(57)90083-1) / Virology / Recombination between related temperate bacteriophages and the genetic control of immunity and prophage localization by Kaiser A. D. (1957)
  25. Kirby , E. P. , F. Jacob , and D. A. Goldwaith . 1967 . Prophage induction and filament formation in a mutant strain of Escherichia coli . Proc. Nat. Acad. Sci. U.S.A. 58 : 1903 - 1910 . (10.1073/pnas.58.5.1903) / Proc. Nat. Acad. Sci. U.S.A. / Prophage induction and filament formation in a mutant strain of Escherichia coli by Kirby E. P. (1967)
  26. Nagai , K. , H. Kaneko , and G. Tamura . 1971 . Thermosensitive mutants of Escherichia coli requiring new protein synthesis to recover cellular division ability . Biochem. Biophys. Res. Commun. 42 : 669 - 675 . (10.1016/0006-291X(71)90540-7) / Biochem. Biophys. Res. Commun. / Thermosensitive mutants of Escherichia coli requiring new protein synthesis to recover cellular division ability by Nagai K. (1971)
  27. Nagel De Zwaig , R. , and S. Luria . 1967 . Genetics and physiology of colicin-tolerant mutants of Escherichia coli . J. Bacteriol. 94 : 1112 - 1123 . (10.1128/jb.94.4.1112-1123.1967) / J. Bacteriol. / Genetics and physiology of colicin-tolerant mutants of Escherichia coli by Nagel De Zwaig R. (1967)
  28. Piechaud , M. 1954 . La coloration sans hydrolyse du noyau des bacteries . Ann. Inst. Pasteur 86 : 787 - 793 . / Ann. Inst. Pasteur / La coloration sans hydrolyse du noyau des bacteries by Piechaud M. (1954)
  29. Reeves , J. , and D. J. Clark . 1972 . Cell division of Escherichia coli BUG6: effect of varying the length of growth at the non permissive growth condition . J. Bacteriol. 110 : 122 - 125 . (10.1128/jb.110.1.122-125.1972) / J. Bacteriol. / Cell division of Escherichia coli BUG6: effect of varying the length of growth at the non permissive growth condition by Reeves J. (1972)
  30. Reeves , J. D. , D. Groves , and D. J. Clark . 1970 . Regulation of cell division in Escherichia coli: characterization of temperature-sensitive division mutants . J. Bacteriol. 104 : 1052 - 1064 . (10.1128/jb.104.3.1052-1064.1970) / J. Bacteriol. / Regulation of cell division in Escherichia coli: characterization of temperature-sensitive division mutants by Reeves J. D. (1970)
  31. Ricard , M. , and Y. Hirota . 1969 . Effet des sels sur le processus de division cellulaire d'E. coli . C. R. Acad. Sci. 268 : 1335 - 1338 . / C. R. Acad. Sci. / Effet des sels sur le processus de division cellulaire d'E. coli by Ricard M. (1969)
  32. Ricard M. and Y. Hirota. 1973. Effet osmotique sur le phenotype de mutants thermosensibles d'E. coli. Ann. Inst. Pasteur 124 A:29-43.
  33. Ricard , M. , Y. Hirota , and F. Jacob . 1970 . Isolement de mutants de membrane chez Escherichia coli . C. R. Acad. Sci. 270 : 2591 - 2593 . / C. R. Acad. Sci. / Isolement de mutants de membrane chez Escherichia coli by Ricard M. (1970)
  34. Schwarz , U. , A. Asmus , and H. Frank . 1969 . Autolytic enzymes and cell division of Escherichia coli . J. Mol. Biol. 41 : 419 - 429 . (10.1016/0022-2836(69)90285-X) / J. Mol. Biol. / Autolytic enzymes and cell division of Escherichia coli by Schwarz U. (1969)
  35. Schwarz , U. , and W. Leutgeb . 1971 . Morphogenetic aspects of murein structure and biosynthesis . J. Bacteriol. 106 : 588 - 595 . (10.1128/jb.106.2.588-595.1971) / J. Bacteriol. / Morphogenetic aspects of murein structure and biosynthesis by Schwarz U. (1971)
  36. Shiba , S. , A. Terawaki , T. Taguchi , and J. Kawamata . 1959 . Selective inhibition of formation of deoxyribonucleic acid in Escherichia coli . Nature (London) 183 : 1056 - 1057 . (10.1038/1831056a0) / Nature (London) / Selective inhibition of formation of deoxyribonucleic acid in Escherichia coli by Shiba S. (1959)
  37. Starka , J. , and J. Moravova . 1967 . Phospholipids and cellular division of Escherichia coli . J. Folia Microbiol. 12 : 240 . / J. Folia Microbiol. / Phospholipids and cellular division of Escherichia coli by Starka J. (1967)
  38. Streiblova E. 1970. Scar formation in the course of the life cycle of saccharomyces cerevisiae p. 183-187. In 0. Necas and A. Svoboda (ed.) Proceedings of the international congress-symposium. Yeast protoplasts vol. 2. University J. E. Purkyne Press Prague.
  39. Strominger J. L. 1970. Penicillin-sensitive enzymatic reactions in bacteria cell wall synthesis p. 179-213. Harvey Lectures series 64. Academic Press Inc. New York.
  40. Van De Putte , P. , J. Van Dillewijn , and A. Rorsch . 1964 . The selection of mutants of Escherichia coli with impaired cell division at elevated temperature . Mutat. Res. 1 : 121 - 128 . (10.1016/0027-5107(64)90014-4) / Mutat. Res. / The selection of mutants of Escherichia coli with impaired cell division at elevated temperature by Van De Putte P. (1964)
  41. Weber , G. , and L. E. Young . 1964 . Fragmentation of bovine serum albumine by epsine. I. The origin of the acid expansion of the albumine molecules . J. Biol. Chem. 239 : 1415 - 1423 . (10.1016/S0021-9258(18)91331-1) / J. Biol. Chem. / Fragmentation of bovine serum albumine by epsine. I. The origin of the acid expansion of the albumine molecules by Weber G. (1964)
  42. Witkin , E. 1967 . The radiation sensitivity of Escherichia coli B: a hypothesis relating filament formation and prophage induction . Proc. Nat. Acad. Sci. U.S.A. 57 : 1275 - 1279 . (10.1073/pnas.57.5.1275) / Proc. Nat. Acad. Sci. U.S.A. / The radiation sensitivity of Escherichia coli B: a hypothesis relating filament formation and prophage induction by Witkin E. (1967)
  43. Zusman , D. R. , M. Inouye , and A. B. Pardee . 1972 . Cell division in E. coli: evidence for regulation of septation by effector molecules . J. Mol. Biol. 69 : 119 - 136 . (10.1016/0022-2836(72)90027-7) / J. Mol. Biol. / Cell division in E. coli: evidence for regulation of septation by effector molecules by Zusman D. R. (1972)
Dates
Type When
Created 5 years, 8 months ago (Jan. 3, 2020, 9:54 a.m.)
Deposited 4 years, 2 months ago (June 12, 2021, 4:11 p.m.)
Indexed 1 year, 1 month ago (Aug. 3, 2024, 10:17 p.m.)
Issued 51 years, 11 months ago (Oct. 1, 1973)
Published 51 years, 11 months ago (Oct. 1, 1973)
Published Print 51 years, 11 months ago (Oct. 1, 1973)
Funders 0

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@article{Ricard_1973, title={Process of Cellular Division in Escherichia coli  : Physiological Study on Thermosensitive Mutants Defective in Cell Division}, volume={116}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.116.1.314-322.1973}, DOI={10.1128/jb.116.1.314-322.1973}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Ricard, Matthieu and Hirota, Yukinori}, year={1973}, month=oct, pages={314–322} }