DOI: 10.1128/jb.114.2.603-611.1973. Cell Division of <i>Escherichia coli</i> : Control by Membrane Organization

Cell Division of Escherichia coli : Control by Membrane Organization

10.1128/jb.114.2.603-611.1973

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Cells of certain strains of Escherichia coli , after transfer from 37 to 45 C and incubation for 16 min, were observed to swell and subsequently divide synchronously. This swelling and the resulting stretching of the membrane are proposed to be the basis for the synchronous division. Four lines of evidence support this hypothesis. First, osmotic protection by the addition of either sodium chloride or sucrose at the time of heat shock prevents both swelling and synchrony. Second, a mutant neither swelled nor divided synchronously after heat shock. Third, cells grown for several generations with 10% sucrose in the medium swelled and divided synchronously upon transfer to medium without sucrose. Fourth, the mutant not synchronized by heat shock also swelled and underwent synchronous division after the osmotic shift. A tentative model is suggested for the normal control of division, based on membrane configuration at the septation site.

Bibliography

Wu, P. C., & Pardee, A. B. (1973). Cell Division of Escherichia coli â¯: Control by Membrane Organization. Journal of Bacteriology, 114(2), 603â611.

Authors 2

Po C. Wu (first)
Arthur B. Pardee (additional)

References 17 Referenced 16

Burton K. 1968. Determination of DNA concentration with diphenylamine p. 163-166. In L. Grossman and K. Holdane (ed.) Methods in enzymology vol. 12B. Academic Press Inc. New York. (10.1016/0076-6879(67)12127-7)
Clark , D. J. 1968 . The regulation of deoxyribonucleic acid replication and cell division in Escherichia coli B/r . J. Bacteriol. 96 : 1214 - 1224 . (10.1128/jb.96.4.1214-1224.1968) / J. Bacteriol. / The regulation of deoxyribonucleic acid replication and cell division in Escherichia coli B/r by Clark D. J. (1968)
Clark , D. J. 1968 . The regulation of DNA replication and cell division in E. coli B/r . Cold Spring Harbor Symp. Quant. Biol. 33 : 823 - 838 . (10.1101/SQB.1968.033.01.094) / Cold Spring Harbor Symp. Quant. Biol. / The regulation of DNA replication and cell division in E. coli B/r by Clark D. J. (1968)
Donachie , W. D. 1969 . Control of cell division in Escherichia coli, experiments with thymine starvation . J. Bacteriol. 100 : 260 - 268 . (10.1128/JB.100.1.260-268.1969) / J. Bacteriol. / Control of cell division in Escherichia coli, experiments with thymine starvation by Donachie W. D. (1969)
Donachie , W. D. , and K. J. Begg . 1970 . Growth of the bacterial cell . Nature (London) 227 : 1220 - 1224 . (10.1038/2271220a0) / Nature (London) / Growth of the bacterial cell by Donachie W. D. (1970)
Gross , J. D. 1971 . DNA replication in bacteria . Curr. Top. Microbiol. Immunol. 57 : 39 - 74 . / Curr. Top. Microbiol. Immunol. / DNA replication in bacteria by Gross J. D. (1971)
Helmstetter , C. E. , and 0. Pierucci . 1968 . Cell division during inhibition of deoxyribonucleic acid synthesis in Escherichia coli . J. Bacteriol. 95 : 1627 - 1633 . (10.1128/jb.95.5.1627-1633.1968) / J. Bacteriol. / Cell division during inhibition of deoxyribonucleic acid synthesis in Escherichia coli by Helmstetter C. E. (1968)
Higgins , M. L. , and G. D. Shockman . 1971 . Procaryotic cell division with respect to wall and membrane . CRC Crit. Rev. Microbiol. 1 : 29 - 72 . (10.3109/10408417109104477) / CRC Crit. Rev. Microbiol. / Procaryotic cell division with respect to wall and membrane by Higgins M. L. (1971)
Inouye , M. 1969 . Unlinking of cell division from deoxyribonucleic acid replication on a temperature-sensitive deoxyribonucleic acid synthesis mutant of Escherichia coli . J. Bacteriol. 99 : 842 - 850 . (10.1128/jb.99.3.842-850.1969) / J. Bacteriol. / Unlinking of cell division from deoxyribonucleic acid replication on a temperature-sensitive deoxyribonucleic acid synthesis mutant of Escherichia coli by Inouye M. (1969)
Inouye , M. , and A. B. Pardee . 1970 . A requirement of polyamines for bacterial division . J. Bacteriol. 101 : 770 - 776 . (10.1128/JB.101.3.770-776.1970) / J. Bacteriol. / A requirement of polyamines for bacterial division by Inouye M. (1970)
Lomnitzer , R. , and E. Z. Ron . 1972 . Synchronization of cell division in Escherichia coli by elevated temperatures: a reinterpretation . J. Bacteriol. 109 : 1316 - 1318 . (10.1128/JB.109.3.1316-1318.1972) / J. Bacteriol. / Synchronization of cell division in Escherichia coli by elevated temperatures: a reinterpretation by Lomnitzer R. (1972)
Lowry , 0. H. , N. J. Rosebrough , A. L. Farr , and R. J. Randall . 1951 . Protein measurement with the Folin phenol reagent . J. Biol. Chem. 193 : 265 - 275 . (10.1016/S0021-9258(19)52451-6) / J. Biol. Chem. / Protein measurement with the Folin phenol reagent by Lowry 0. (1951)
Pierucci , O. , and C. E. Helmatetter . 1969 . Chromosome replication, protein synthesis and cell division in E . Coli B/r. Fed. Proc. 28 : 1755 - 1770 . / Coli B/r. Fed. Proc. / Chromosome replication, protein synthesis and cell division in E by Pierucci O. (1969)
Ron , E. Z. , and M. Shani . 1971 . Growth rate of Escherichia coli at elevated temperature: reversible inhibition of homoserine trans-succinylase . J. Bacteriol. 107 : 397 - 400 . (10.1128/jb.107.2.397-400.1971) / J. Bacteriol. / Growth rate of Escherichia coli at elevated temperature: reversible inhibition of homoserine trans-succinylase by Ron E. Z. (1971)
Smith , H. S. , and A. B. Pardee . 1970 . Accumulation of a protein required for division during the cell cycle of Escherichia coli . J. Bacteriol. 101 : 901 - 909 . (10.1128/JB.101.3.901-909.1970) / J. Bacteriol. / Accumulation of a protein required for division during the cell cycle of Escherichia coli by Smith H. S. (1970)
Van de Putte , P. , J. van Dillewijn , and A. Rorsch . 1964 . The selection of mutants of Escherichia coli with impaired cell division at elevated temperature . Mutat. Res. 1 : 121 - 128 . (10.1016/0027-5107(64)90014-4) / Mutat. Res. / The selection of mutants of Escherichia coli with impaired cell division at elevated temperature by Van de Putte P. (1964)
Webb , M. 1953 . Effects of magnesium on cellular division in bacteria . Science 118 : 607 - 611 . (10.1126/science.118.3073.607) / Science / Effects of magnesium on cellular division in bacteria by Webb M. (1953)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 9:42 a.m.)
Deposited	4 years ago (July 29, 2021, 1:09 p.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 3:33 p.m.)
Issued	52 years, 3 months ago (May 1, 1973)
Published	52 years, 3 months ago (May 1, 1973)
Published Print	52 years, 3 months ago (May 1, 1973)

Funders 0

None

BibTeX

@article{Wu_1973, title={Cell Division of Escherichia coli  : Control by Membrane Organization}, volume={114}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.114.2.603-611.1973}, DOI={10.1128/jb.114.2.603-611.1973}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Wu, Po C. and Pardee, Arthur B.}, year={1973}, month=may, pages={603–611} }

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