Synthesis and Structure of Caulobacter crescentus Flagella
10.1128/jb.113.1.478-485.1973
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

During the normal cell cycle of Caulobacter crescentus , flagella are released into the culture fluid as swarmer cells differentiate into stalked cells. The released flagellum is composed of a filament, hook, and rod. The molecular weight of purified flagellin (subunit of flagella filament) is 25,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The formation of a flagellum opposite the stalk has been observed by microscope during the differentiation of a stalked cell in preparation for cell division. By pulsing synchronized cultures with 14 C-amino acids it has been demonstrated that the synthesis of flagellin occurs approximately 30 to 40 min before cell division. Flagellin, therefore, is synthesized at a discrete time in the cell cycle and is assembled into flagella at a specific site on the cell. A mutant of C. crescentus that fails to synthesize flagellin has been isolated.

Bibliography

Shapiro, L., & Maizel, J. V. (1973). Synthesis and Structure of Caulobacter crescentus Flagella. Journal of Bacteriology, 113(1), 478–485.

Authors 2
  1. Lucille Shapiro (first)
  2. J. V. Maizel (additional)
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Dates
Type When
Created 5 years, 7 months ago (Jan. 3, 2020, 9:40 a.m.)
Deposited 4 years, 3 months ago (May 26, 2021, 8:45 a.m.)
Indexed 2 years, 1 month ago (July 13, 2023, 1:12 p.m.)
Issued 52 years, 7 months ago (Jan. 1, 1973)
Published 52 years, 7 months ago (Jan. 1, 1973)
Published Print 52 years, 7 months ago (Jan. 1, 1973)
Funders 0

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@article{Shapiro_1973, title={Synthesis and Structure of Caulobacter crescentus Flagella}, volume={113}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.113.1.478-485.1973}, DOI={10.1128/jb.113.1.478-485.1973}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Shapiro, Lucille and Maizel, J. V.}, year={1973}, month=jan, pages={478–485} }