DOI: 10.1128/jb.112.1.519-526.1972. Effect of <i>p</i> H on Adenine and Amino Acid Uptake During Sporulation in <i>Saccharomyces cerevisiae</i>

Effect of p H on Adenine and Amino Acid Uptake During Sporulation in Saccharomyces cerevisiae

10.1128/jb.112.1.519-526.1972

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

During the early stages of sporulation in Saccharomyces cerevisiae , the p H of the acetate sporulation medium rises to values of 8.0 or higher. Associated with this rise in p H is a reduced cell permeability to certain precursors of ribonucleic acid (RNA), deoxyribonucleic acid or protein. Uptake of adenine, alanine, and leucine was optimal at p H 5.6 to 6.0, but sporulation was inhibited when the sporulation medium was buffered below p H 7.0. Cellular impermeability can be largely overcome by adjusting the acetate sporulation medium to p H 6.0 for optimal uptake of 14 C-adenine during short pulses without any apparent effect on sporulation. Sporulating cells pulse-labeled 20 min at p H 6.0 incorporated 40 times more 14 C-adenine into RNA than sporulating cells pulse-labeled at p H 8.0. This increased incorporation can be attributed to a 100-fold increase in labeled adenosine triphosphate in cells pulse-labeled at p H 6.0 where maximum uptake occurs.

Bibliography

Mills, D. (1972). Effect of p H on Adenine and Amino Acid Uptake During Sporulation in Saccharomyces cerevisiae. Journal of Bacteriology, 112(1), 519â526.

Authors 1

Dallice Mills (first)

References 17 Referenced 42

Ames , B. N. , and D. T. Dubin . 1960 . The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid . J. Biol. Chem. 235 : 769 - 775 . (10.1016/S0021-9258(19)67936-6) / J. Biol. Chem. / The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid by Ames B. N. (1960)
Cashel , M. , and J. Gallant . 1968 . Control of RNA synthesis in Escherichia coli . J. Mol. Biol. 34 : 317 - 330 . (10.1016/0022-2836(68)90256-8) / J. Mol. Biol. / Control of RNA synthesis in Escherichia coli by Cashel M. (1968)
Chapman , A. G. , L. Fall , and D. E. Atkinson . 1971 . Adenylate energy charge in Escherichia coli during growth and starvation . J. Bacteriol. 108 : 1072 - 1086 . (10.1128/jb.108.3.1072-1086.1971) / J. Bacteriol. / Adenylate energy charge in Escherichia coli during growth and starvation by Chapman A. G. (1971)
Cole , H. A. , J. W. T. Wimpenny , and D. E. Hughes . 1967 . The ATP pool in Escherichia coli. I. Measurement of the pool using a modified Iuciferase assay . Biochim. Biophys. Acta 143 : 445 - 453 . (10.1016/0005-2728(67)90050-3) / Biochim. Biophys. Acta / The ATP pool in Escherichia coli. I. Measurement of the pool using a modified Iuciferase assay by Cole H. A. (1967)
Croes , A. F. 1966 . Duplication of DNA during meiosis in baker's yeast . Exp. Cell Res. 41 : 452 - 454 . (10.1016/S0014-4827(66)80151-9) / Exp. Cell Res. / Duplication of DNA during meiosis in baker's yeast by Croes A. F. (1966)
Croes , A. F. 1967 . Induction of meiosis in yeast. I. Timing of cytological and biochemical events . Planta 76 : 209 - 226 . / Planta / Induction of meiosis in yeast. I. Timing of cytological and biochemical events by Croes A. F. (1967)
Duggan , P. F. 1964 . The uptake of potassium by baker's yeast from potassium acetate . Biochim. Biophys. Acta 88 : 223 - 224 . / Biochim. Biophys. Acta / The uptake of potassium by baker's yeast from potassium acetate by Duggan P. F. (1964)
Eaton , N. R. 1962 . New press for disruption of microorganisms . J. Bacteriol. 83 : 1359 - 1360 . (10.1128/jb.83.6.1359-1360.1962) / J. Bacteriol. / New press for disruption of microorganisms by Eaton N. R. (1962)
Esposito , M. S. , R. E. Esposito , M. Arnaud , and H. 0. Halvorson . 1969 . Acetate utilization and macromolecular synthesis during sporulation of yeast . J. Bacteriol. 100 : 180 - 186 . (10.1128/jb.100.1.180-186.1969) / J. Bacteriol. / Acetate utilization and macromolecular synthesis during sporulation of yeast by Esposito M. S. (1969)
Esposito , M. S. , R. E. Esposito , M. Arnaud , and H. 0. Halvorson . 1970 . Conditional mutants of meiosis in yeast . J. Bacteriol. 104 : 202 - 210 . (10.1128/jb.104.1.202-210.1970) / J. Bacteriol. / Conditional mutants of meiosis in yeast by Esposito M. S. (1970)
Kadowaki , K. , and H. 0. Halvorson . 1971 . Appearance of a new species of ribonucleic acid during sporulation in Saccharomyces cereuisiae . J. Bacteriol. 105 : 826 - 830 . (10.1128/jb.105.3.826-830.1971) / J. Bacteriol. / Appearance of a new species of ribonucleic acid during sporulation in Saccharomyces cereuisiae by Kadowaki K. (1971)
Kadowaki , K. , and H. 0. Halvorson . 1971 . Isolation and properties of a new species of ribonucleic acid synthesized in sporulating cells of Saccharomyces cerevisiae . J. Bacteriol. 105 : 831 - 836 . (10.1128/jb.105.3.831-836.1971) / J. Bacteriol. / Isolation and properties of a new species of ribonucleic acid synthesized in sporulating cells of Saccharomyces cerevisiae by Kadowaki K. (1971)
Randerath , K. , and E. Randerath . 1964 . Ion-exchange chromatography of nucleotides on poly-(ethylene-imine)-cellulose thin layers . J. Chromatogr. 16 : 111 - 126 . (10.1016/S0021-9673(01)82445-6) / J. Chromatogr. / Ion-exchange chromatography of nucleotides on poly-(ethylene-imine)-cellulose thin layers by Randerath K. (1964)
Randerath , E. , and K. Randerath . 1964 . Resolution of complex nucleotide mixtures by two dimensional anion-exchange thin layer chromatography . J. Chromatogr. 16 : 126 - 129 . (10.1016/S0021-9673(01)82446-8) / J. Chromatogr. / Resolution of complex nucleotide mixtures by two dimensional anion-exchange thin layer chromatography by Randerath E. (1964)
Rodenberg , S. , W. Steinberg , J. Piper , K. Nickerson , J. Vary , R. Epstein , and H. 0. Halvorson . 1968 . Relationship between protein and ribonucleic acid synthesis during outgrowth of spores of Bacillus cereus . J. Bacteriol. 96 : 492 - 500 . (10.1128/jb.96.2.492-500.1968) / J. Bacteriol. / Relationship between protein and ribonucleic acid synthesis during outgrowth of spores of Bacillus cereus by Rodenberg S. (1968)
Roth , R. , and K. Lusnak . 1970 . DNA synthesis during yeast sporulation: genetic control of an early developmental event . Science 168 : 493 - 494 . (10.1126/science.168.3930.493) / Science / DNA synthesis during yeast sporulation: genetic control of an early developmental event by Roth R. (1970)
Tisdale , J. H. , and A. G. DeBusk . 1970 . Developmental regulation of amino acid transport in Neurospora . J. Bacteriol. 104 : 689 - 697 . (10.1128/jb.104.2.689-697.1970) / J. Bacteriol. / Developmental regulation of amino acid transport in Neurospora by Tisdale J. H. (1970)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 9:38 a.m.)
Deposited	4 years, 2 months ago (June 13, 2021, 1:35 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 1:57 p.m.)
Issued	52 years, 11 months ago (Oct. 1, 1972)
Published	52 years, 11 months ago (Oct. 1, 1972)
Published Print	52 years, 11 months ago (Oct. 1, 1972)

Funders 0

None

BibTeX

@article{Mills_1972, title={Effect of p H on Adenine and Amino Acid Uptake During Sporulation in Saccharomyces cerevisiae}, volume={112}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.112.1.519-526.1972}, DOI={10.1128/jb.112.1.519-526.1972}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Mills, Dallice}, year={1972}, month=oct, pages={519–526} }

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