DOI: 10.1128/jb.109.2.520-525.1972. Leakage of Periplasmic Enzymes by Mutants of <i>Escherichia coli</i> and <i>Salmonella typhimurium:</i> Isolation of “Periplasmic Leaky” Mutants

Leakage of Periplasmic Enzymes by Mutants of Escherichia coli and Salmonella typhimurium: Isolation of “Periplasmic Leaky” Mutants

10.1128/jb.109.2.520-525.1972

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Mutants of Escherichia coli and Salmonella typhimurium were selected on the basis of their spontaneous leakage of ribonuclease I. The mutants also leaked several other periplasmic enzymes into the medium during active growth but did not leak the intracellular enzymes glucose-6-phosphate dehydrogenase or phosphoglucose isomerase.

Bibliography

Lopes, J., Gottfried, S., & Rothfield, L. (1972). Leakage of Periplasmic Enzymes by Mutants of Escherichia coli and Salmonella typhimurium: Isolation of âPeriplasmic Leakyâ Mutants. Journal of Bacteriology, 109(2), 520â525.

Authors 3

J. Lopes (first)
S. Gottfried (additional)
L. Rothfield (additional)

References 18 Referenced 80

Ames , G. , and J. Lever . 1970 . Components of histidine transport: histidine-binding proteins and his P proteins . Proc. Nat. Acad. Sci. U.S.A. 66 : 1096 - 1103 . (10.1073/pnas.66.4.1096) / Proc. Nat. Acad. Sci. U.S.A. / Components of histidine transport: histidine-binding proteins and his P proteins by Ames G. (1970)
Anraku , Y. , and L. A. Heppel . 1967 . On the nature of the changes induced in Escherichia coli by osmotic shock . J. Biol. Chem. 242 : 2561 - 2569 . (10.1016/S0021-9258(18)96000-X) / J. Biol. Chem. / On the nature of the changes induced in Escherichia coli by osmotic shock by Anraku Y. (1967)
Eidels , L. , and M. J. Osborn . 1971 . Lipopolysaccharide and aldoheptose biosynthesis in transketolase mutants of Salmonella typhimurium . Proc. Nat. Acad. Sci. U.S.A. 68 : 1673 - 1677 . (10.1073/pnas.68.8.1673) / Proc. Nat. Acad. Sci. U.S.A. / Lipopolysaccharide and aldoheptose biosynthesis in transketolase mutants of Salmonella typhimurium by Eidels L. (1971)
Garen , A. , and C. Levinthal . 1960 . A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase . Biochim. Biophys. Acta 38 : 470 - 483 . (10.1016/0006-3002(60)91282-8) / Biochim. Biophys. Acta / A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase by Garen A. (1960)
Gesteland , R. F. 1966 . Isolation and characterization of ribonuclease I mutants of Escherichia coli . J. Mol. Biol. 16 : 67 - 84 . (10.1016/S0022-2836(66)80263-2) / J. Mol. Biol. / Isolation and characterization of ribonuclease I mutants of Escherichia coli by Gesteland R. F. (1966)
Heppel L. A. 1971. The concept of periplasmic enzymes in bacteria p. 223-247. In L. I. Rothfield (ed.) The structure and function of biological membranes. Academic Press Inc. New York. (10.1016/B978-0-12-598650-2.50010-7)
Langdon R. G. 1966. Glucose-6-phosphate dehydrogenase from erythrocytes section 3 p. 126-131. In W. A. Ward (ed.) Methods of enzymology vol. 9 Academic Press Inc. New York. (10.1016/0076-6879(66)09030-X)
Lennox , E. S. 1955 . Transduction of linked genetic character of the host by bacteriophage P1 . Virology 1 : 190 - 206 . (10.1016/0042-6822(55)90016-7) / Virology / Transduction of linked genetic character of the host by bacteriophage P1 by Lennox E. S. (1955)
Nagel de Zwaig , R. , and S. E. Luria . 1967 . Genetics and physiology of colicin-tolerant mutants of Escherichia coli . J. Bacteriol. 94 : 1112 - 1123 . (10.1128/jb.94.4.1112-1123.1967) / J. Bacteriol. / Genetics and physiology of colicin-tolerant mutants of Escherichia coli by Nagel de Zwaig R. (1967)
Neu , H. C. , and L. A. Heppel . 1965 . The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts . J. Biol. Chem. 240 : 3685 - 3692 . (10.1016/S0021-9258(18)97200-5) / J. Biol. Chem. / The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts by Neu H. C. (1965)
Noltmann E. A. 1966. Phosphoglucose isomerase I. Rabbit muscle (crystalline) section 6 p. 557-565. In Willis A. Wood (ed.) Methods of enzymology vol. 9 Academic Press Inc. New York. (10.1016/0076-6879(66)09111-0)
Nossal , N. G. , and L. A. Heppel . 1966 . The release of enzymes by osmotic shock from Escherichia coli in exponential phase . J. Biol. Chem. 241 : 3055 - 3062 . (10.1016/S0021-9258(18)96497-5) / J. Biol. Chem. / The release of enzymes by osmotic shock from Escherichia coli in exponential phase by Nossal N. G. (1966)
Osborn , M. J. , S. M. Rosen . L. Rothfield , and B. L. Horecker . 1962 . Biosynthesis of bacterial lipopolysaccharide. I. Enzymatic incorporation of galactose in a mutant strain of Salmonella . Proc. Nat. Acad. Sci. U.S.A. 48 : 1831 - 1838 . (10.1073/pnas.48.10.1831) / Proc. Nat. Acad. Sci. U.S.A. / Biosynthesis of bacterial lipopolysaccharide. I. Enzymatic incorporation of galactose in a mutant strain of Salmonella by Osborn M. J. (1962)
Pestka , S. 1968 . Studies on the formation of tRNA-ribosome complexes III . J. Biol. Chem. 243 : 2810 - 2820 . (10.1016/S0021-9258(18)93445-9) / J. Biol. Chem. / Studies on the formation of tRNA-ribosome complexes III by Pestka S. (1968)
Schlecht , S. , and 0. Westphal . 1968 . Antiobiotica-Empfindlichkeit bei S- und R-Formen von Salmonella minnesota . Naturwissenschaften 10 : 494 - 495 . (10.1007/BF00599721) / Naturwissenschaften / Antiobiotica-Empfindlichkeit bei S- und R-Formen von Salmonella minnesota by Schlecht S. (1968)
Tamaki , S. , T. Sato , and M. Matsuhashi . 1971 . Role of lipopolysaccharides in antibiotic resistance and bacteriophage adsorption of Escherichia coli K-12 . J. Bacteriol. 105 : 968 - 975 . (10.1128/jb.105.3.968-975.1971) / J. Bacteriol. / Role of lipopolysaccharides in antibiotic resistance and bacteriophage adsorption of Escherichia coli K-12 by Tamaki S. (1971)
Vogel , H. J. , and D. M. Bonner . 1956 . Acetylornithinase of Escherichia coli: partial purification and some properties . J. Biol. Chem. 218 : 97 - 106 . (10.1016/S0021-9258(18)65874-0) / J. Biol. Chem. / Acetylornithinase of Escherichia coli: partial purification and some properties by Vogel H. J. (1956)
Wetzel , B. K. , S. S. Spicer , H. F. Dvorak , and L. A. Heppel . 1970 . Cytochemical localization of certain phosphatases in Escherichia coli . J. Bacteriol. 104 : 529 - 542 . (10.1128/jb.104.1.529-542.1970) / J. Bacteriol. / Cytochemical localization of certain phosphatases in Escherichia coli by Wetzel B. K. (1970)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 9:34 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1 p.m.)
Indexed	2 months, 3 weeks ago (June 13, 2025, 3:28 a.m.)
Issued	53 years, 7 months ago (Feb. 1, 1972)
Published	53 years, 7 months ago (Feb. 1, 1972)
Published Print	53 years, 7 months ago (Feb. 1, 1972)

Funders 0

None

BibTeX

@article{Lopes_1972, title={Leakage of Periplasmic Enzymes by Mutants of Escherichia coli and Salmonella typhimurium: Isolation of “Periplasmic Leaky” Mutants}, volume={109}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.109.2.520-525.1972}, DOI={10.1128/jb.109.2.520-525.1972}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Lopes, J. and Gottfried, S. and Rothfield, L.}, year={1972}, month=feb, pages={520–525} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        6,
        13
      ]
    ],
    "date-time": "2025-06-13T07:28:09Z",
    "timestamp": 1749799689494
  },
  "reference-count": 18,
  "publisher": "American Society for Microbiology",
  "issue": "2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1972,
            2,
            1
          ]
        ],
        "date-time": "1972-02-01T00:00:00Z",
        "timestamp": 65750400000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1972,
        2
      ]
    ]
  },
  "abstract": "<jats:p>\n            Mutants of\n            <jats:italic>Escherichia coli</jats:italic>\n            and\n            <jats:italic>Salmonella typhimurium</jats:italic>\n            were selected on the basis of their spontaneous leakage of ribonuclease I. The mutants also leaked several other periplasmic enzymes into the medium during active growth but did not leak the intracellular enzymes glucose-6-phosphate dehydrogenase or phosphoglucose isomerase.\n          </jats:p>",
  "DOI": "10.1128/jb.109.2.520-525.1972",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2020,
        1,
        3
      ]
    ],
    "date-time": "2020-01-03T14:34:45Z",
    "timestamp": 1578062085000
  },
  "page": "520-525",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 80,
  "title": "Leakage of Periplasmic Enzymes by Mutants of\n            <i>Escherichia coli</i>\n            and\n            <i>Salmonella typhimurium:</i>\n            Isolation of \u201cPeriplasmic Leaky\u201d Mutants",
  "prefix": "10.1128",
  "volume": "109",
  "author": [
    {
      "given": "J.",
      "family": "Lopes",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Microbiology, University of Connecticut Health Center, Farmington, Connecticut 06032"
        }
      ]
    },
    {
      "given": "S.",
      "family": "Gottfried",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Microbiology, University of Connecticut Health Center, Farmington, Connecticut 06032"
        }
      ]
    },
    {
      "given": "L.",
      "family": "Rothfield",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Microbiology, University of Connecticut Health Center, Farmington, Connecticut 06032"
        }
      ]
    }
  ],
  "member": "235",
  "reference": [
    {
      "key": "p_1",
      "doi-asserted-by": "crossref",
      "first-page": "1096",
      "DOI": "10.1073/pnas.66.4.1096",
      "article-title": "Components of histidine transport: histidine-binding proteins and his P proteins",
      "volume": "66",
      "author": "Ames G.",
      "year": "1970",
      "unstructured": "Ames , G. , and J. Lever . 1970 . Components of histidine transport: histidine-binding proteins and his P proteins . Proc. Nat. Acad. Sci. U.S.A. 66 : 1096 - 1103 .",
      "journal-title": "Proc. Nat. Acad. Sci. U.S.A."
    },
    {
      "key": "p_2",
      "doi-asserted-by": "crossref",
      "first-page": "2561",
      "DOI": "10.1016/S0021-9258(18)96000-X",
      "article-title": "On the nature of the changes induced in Escherichia coli by osmotic shock",
      "volume": "242",
      "author": "Anraku Y.",
      "year": "1967",
      "unstructured": "Anraku , Y. , and L. A. Heppel . 1967 . On the nature of the changes induced in Escherichia coli by osmotic shock . J. Biol. Chem. 242 : 2561 - 2569 .",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "p_3",
      "doi-asserted-by": "crossref",
      "first-page": "1673",
      "DOI": "10.1073/pnas.68.8.1673",
      "article-title": "Lipopolysaccharide and aldoheptose biosynthesis in transketolase mutants of Salmonella typhimurium",
      "volume": "68",
      "author": "Eidels L.",
      "year": "1971",
      "unstructured": "Eidels , L. , and M. J. Osborn . 1971 . Lipopolysaccharide and aldoheptose biosynthesis in transketolase mutants of Salmonella typhimurium . Proc. Nat. Acad. Sci. U.S.A. 68 : 1673 - 1677 .",
      "journal-title": "Proc. Nat. Acad. Sci. U.S.A."
    },
    {
      "key": "p_4",
      "doi-asserted-by": "crossref",
      "first-page": "470",
      "DOI": "10.1016/0006-3002(60)91282-8",
      "article-title": "A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase",
      "volume": "38",
      "author": "Garen A.",
      "year": "1960",
      "unstructured": "Garen , A. , and C. Levinthal . 1960 . A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase . Biochim. Biophys. Acta 38 : 470 - 483 .",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "p_5",
      "doi-asserted-by": "crossref",
      "first-page": "67",
      "DOI": "10.1016/S0022-2836(66)80263-2",
      "article-title": "Isolation and characterization of ribonuclease I mutants of Escherichia coli",
      "volume": "16",
      "author": "Gesteland R. F.",
      "year": "1966",
      "unstructured": "Gesteland , R. F. 1966 . Isolation and characterization of ribonuclease I mutants of Escherichia coli . J. Mol. Biol. 16 : 67 - 84 .",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "p_6",
      "doi-asserted-by": "crossref",
      "unstructured": "Heppel L. A. 1971. The concept of periplasmic enzymes in bacteria p. 223-247. In L. I. Rothfield (ed.) The structure and function of biological membranes. Academic Press Inc. New York.",
      "DOI": "10.1016/B978-0-12-598650-2.50010-7"
    },
    {
      "key": "p_7",
      "doi-asserted-by": "crossref",
      "unstructured": "Langdon R. G. 1966. Glucose-6-phosphate dehydrogenase from erythrocytes section 3 p. 126-131. In W. A. Ward (ed.) Methods of enzymology vol. 9 Academic Press Inc. New York.",
      "DOI": "10.1016/0076-6879(66)09030-X"
    },
    {
      "key": "p_8",
      "doi-asserted-by": "crossref",
      "first-page": "190",
      "DOI": "10.1016/0042-6822(55)90016-7",
      "article-title": "Transduction of linked genetic character of the host by bacteriophage P1",
      "volume": "1",
      "author": "Lennox E. S.",
      "year": "1955",
      "unstructured": "Lennox , E. S. 1955 . Transduction of linked genetic character of the host by bacteriophage P1 . Virology 1 : 190 - 206 .",
      "journal-title": "Virology"
    },
    {
      "key": "p_9",
      "doi-asserted-by": "crossref",
      "first-page": "1112",
      "DOI": "10.1128/jb.94.4.1112-1123.1967",
      "article-title": "Genetics and physiology of colicin-tolerant mutants of Escherichia coli",
      "volume": "94",
      "author": "Nagel de Zwaig R.",
      "year": "1967",
      "unstructured": "Nagel de Zwaig , R. , and S. E. Luria . 1967 . Genetics and physiology of colicin-tolerant mutants of Escherichia coli . J. Bacteriol. 94 : 1112 - 1123 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_10",
      "doi-asserted-by": "crossref",
      "first-page": "3685",
      "DOI": "10.1016/S0021-9258(18)97200-5",
      "article-title": "The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts",
      "volume": "240",
      "author": "Neu H. C.",
      "year": "1965",
      "unstructured": "Neu , H. C. , and L. A. Heppel . 1965 . The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts . J. Biol. Chem. 240 : 3685 - 3692 .",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "p_11",
      "doi-asserted-by": "crossref",
      "unstructured": "Noltmann E. A. 1966. Phosphoglucose isomerase I. Rabbit muscle (crystalline) section 6 p. 557-565. In Willis A. Wood (ed.) Methods of enzymology vol. 9 Academic Press Inc. New York.",
      "DOI": "10.1016/0076-6879(66)09111-0"
    },
    {
      "key": "p_12",
      "doi-asserted-by": "crossref",
      "first-page": "3055",
      "DOI": "10.1016/S0021-9258(18)96497-5",
      "article-title": "The release of enzymes by osmotic shock from Escherichia coli in exponential phase",
      "volume": "241",
      "author": "Nossal N. G.",
      "year": "1966",
      "unstructured": "Nossal , N. G. , and L. A. Heppel . 1966 . The release of enzymes by osmotic shock from Escherichia coli in exponential phase . J. Biol. Chem. 241 : 3055 - 3062 .",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "p_13",
      "doi-asserted-by": "crossref",
      "first-page": "1831",
      "DOI": "10.1073/pnas.48.10.1831",
      "article-title": "Biosynthesis of bacterial lipopolysaccharide. I. Enzymatic incorporation of galactose in a mutant strain of Salmonella",
      "volume": "48",
      "author": "Osborn M. J.",
      "year": "1962",
      "unstructured": "Osborn , M. J. , S. M. Rosen . L. Rothfield , and B. L. Horecker . 1962 . Biosynthesis of bacterial lipopolysaccharide. I. Enzymatic incorporation of galactose in a mutant strain of Salmonella . Proc. Nat. Acad. Sci. U.S.A. 48 : 1831 - 1838 .",
      "journal-title": "Proc. Nat. Acad. Sci. U.S.A."
    },
    {
      "key": "p_14",
      "doi-asserted-by": "crossref",
      "first-page": "2810",
      "DOI": "10.1016/S0021-9258(18)93445-9",
      "article-title": "Studies on the formation of tRNA-ribosome complexes III",
      "volume": "243",
      "author": "Pestka S.",
      "year": "1968",
      "unstructured": "Pestka , S. 1968 . Studies on the formation of tRNA-ribosome complexes III . J. Biol. Chem. 243 : 2810 - 2820 .",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "p_15",
      "doi-asserted-by": "crossref",
      "first-page": "494",
      "DOI": "10.1007/BF00599721",
      "article-title": "Antiobiotica-Empfindlichkeit bei S- und R-Formen von Salmonella minnesota",
      "volume": "10",
      "author": "Schlecht S.",
      "year": "1968",
      "unstructured": "Schlecht , S. , and 0. Westphal . 1968 . Antiobiotica-Empfindlichkeit bei S- und R-Formen von Salmonella minnesota . Naturwissenschaften 10 : 494 - 495 .",
      "journal-title": "Naturwissenschaften"
    },
    {
      "key": "p_16",
      "doi-asserted-by": "crossref",
      "first-page": "968",
      "DOI": "10.1128/jb.105.3.968-975.1971",
      "article-title": "Role of lipopolysaccharides in antibiotic resistance and bacteriophage adsorption of Escherichia coli K-12",
      "volume": "105",
      "author": "Tamaki S.",
      "year": "1971",
      "unstructured": "Tamaki , S. , T. Sato , and M. Matsuhashi . 1971 . Role of lipopolysaccharides in antibiotic resistance and bacteriophage adsorption of Escherichia coli K-12 . J. Bacteriol. 105 : 968 - 975 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_17",
      "doi-asserted-by": "crossref",
      "first-page": "97",
      "DOI": "10.1016/S0021-9258(18)65874-0",
      "article-title": "Acetylornithinase of Escherichia coli: partial purification and some properties",
      "volume": "218",
      "author": "Vogel H. J.",
      "year": "1956",
      "unstructured": "Vogel , H. J. , and D. M. Bonner . 1956 . Acetylornithinase of Escherichia coli: partial purification and some properties . J. Biol. Chem. 218 : 97 - 106 .",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "p_18",
      "doi-asserted-by": "crossref",
      "first-page": "529",
      "DOI": "10.1128/jb.104.1.529-542.1970",
      "article-title": "Cytochemical localization of certain phosphatases in Escherichia coli",
      "volume": "104",
      "author": "Wetzel B. K.",
      "year": "1970",
      "unstructured": "Wetzel , B. K. , S. S. Spicer , H. F. Dvorak , and L. A. Heppel . 1970 . Cytochemical localization of certain phosphatases in Escherichia coli . J. Bacteriol. 104 : 529 - 542 .",
      "journal-title": "J. Bacteriol."
    }
  ],
  "container-title": "Journal of Bacteriology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.109.2.520-525.1972",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.109.2.520-525.1972",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        7,
        29
      ]
    ],
    "date-time": "2021-07-29T17:00:59Z",
    "timestamp": 1627578059000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/jb.109.2.520-525.1972"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1972,
        2
      ]
    ]
  },
  "references-count": 18,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1972,
          2
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/jb.109.2.520-525.1972"
  ],
  "URL": "http://dx.doi.org/10.1128/jb.109.2.520-525.1972",
  "relation": {},
  "ISSN": [
    "0021-9193",
    "1098-5530"
  ],
  "subject": [],
  "container-title-short": "J Bacteriol",
  "published": {
    "date-parts": [
      [
        1972,
        2
      ]
    ]
  },
  "assertion": [
    {
      "value": "2021-02-02",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}