DOI: 10.1128/jb.108.3.1045-1050.1971. Use of Yeast Populations Fractionated by Zonal Centrifugation to Study the Cell Cycle

Use of Yeast Populations Fractionated by Zonal Centrifugation to Study the Cell Cycle

10.1128/jb.108.3.1045-1050.1971

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Zonal centrifugation in a sucrose density gradient was used to separate yeast cells primarily by size and thus by age in the cell cycle. This approach provides an alternative to synchronous growth for examining the properties of cells at different stages in the cell cycle.

Bibliography

Sebastian, J., Carter, B. L. A., & Halvorson, H. O. (1971). Use of Yeast Populations Fractionated by Zonal Centrifugation to Study the Cell Cycle. Journal of Bacteriology, 108(3), 1045â1050.

Authors 3

J. Sebastian (first)
B. L. A. Carter (additional)
H. O. Halvorson (additional)

References 17 Referenced 63

Anderson , N. G. , H. P. Barringer , N. Cho , C. E. Nunley , E. F. Babelay , R. E. Canning , and C. T. Rankin , Jr. 1966 . The development of low-speed "A " series zonal rotors. Nat. Cancer. Inst. Monogr. 21 : 113 - 136 . / Nat. Cancer. Inst. Monogr. / The development of low-speed "A by Anderson N. G. (1966)
Burton K. 1968. Determination of DNA concentration with diphenylamine p. 163-166. In L. Grossman and K. Moldave (ed.) Methods in enzymology vol. 12 part B. Academic Press Inc. New York. (10.1016/0076-6879(67)12127-7)
Campbell , A. 1957 . Synchronization of cell division . Bacteriol. Rev. 21 : 263 - 272 . (10.1128/br.21.4.263-272.1957) / Bacteriol. Rev. / Synchronization of cell division by Campbell A. (1957)
Halvorson , H. O. , and L. Ellias . 1958 . The purification and properties of an a-glucosidase of Saccharomyces italicus Y1225 . Biochim. Biophys. Acta 30 : 28 - 40 . (10.1016/0006-3002(58)90237-3) / Biochim. Biophys. Acta / The purification and properties of an a-glucosidase of Saccharomyces italicus Y1225 by Halvorson H. O. (1958)
Halvorson H. O. B. L. A. Carter and P. Tauro. 1971. Use of synchronous cultures of yeast to study gene position. In L. Grossman and K. Moldave (ed.) Methods in enzymology vol. 21. Academic Press Inc. New York. (10.1016/S0076-6879(71)21039-9)
Hartwell , L. H. 1970 . Periodic density fluctuation during the yeast cell cycle and the selection of synchronous cultures . J. Bacteriol. 104 : 1280 - 1285 . (10.1128/jb.104.3.1280-1285.1970) / J. Bacteriol. / Periodic density fluctuation during the yeast cell cycle and the selection of synchronous cultures by Hartwell L. H. (1970)
Kubitschek , H. E. 1970 . Evidence for the generality of linear cell growth . J. Theor. Biol. 28 : 15 - 29 . (10.1016/0022-5193(70)90061-5) / J. Theor. Biol. / Evidence for the generality of linear cell growth by Kubitschek H. E. (1970)
Kubitschek , H. E. , H. E. Bendigkeit , and M. R. Loken . 1967 . Onset of DNA synthesis during the cell cycle in chemostat cultures . Proc. Nat. Acad. Sci. U.S.A. 57 : 1611 - 1617 . (10.1073/pnas.57.6.1611) / Proc. Nat. Acad. Sci. U.S.A. / Onset of DNA synthesis during the cell cycle in chemostat cultures by Kubitschek H. E. (1967)
Manor , H. , and R. Haselkorn . 1967 . Size fractionation of exponentially growing Escherichia coli . Nature (London) 214 : 983 - 986 . (10.1038/214983a0) / Nature (London) / Size fractionation of exponentially growing Escherichia coli by Manor H. (1967)
Middelhoven , W. J. 1964 . The pathway of arginine breakdown in Saccharomyces cerevisiae . Biochim. Biophys. Acta 93 : 650 - 652 . (10.1016/0304-4165(64)90349-6) / Biochim. Biophys. Acta / The pathway of arginine breakdown in Saccharomyces cerevisiae by Middelhoven W. J. (1964)
Mitchison , J. M. 1958 . The growth of single cells. II. Saccharomyces cerevisiae . Exp. Cell Res. 15 : 214 - 221 . (10.1016/0014-4827(58)90077-6) / Exp. Cell Res. / The growth of single cells. II. Saccharomyces cerevisiae by Mitchison J. M. (1958)
Mitchison , J. M. 1969 . Enzyme synthesis in synchronous cultures . Science 165 : 657 - 663 . (10.1126/science.165.3894.657) / Science / Enzyme synthesis in synchronous cultures by Mitchison J. M. (1969)
Ogur , M. , and G. Rosen . 1950 . The nucleic acids of plant tissues. I. The extraction and estimation of deoxypentose nucleic acid and pentose nucleic acid . Arch. Biochem. 25 : 262 - 276 . / Arch. Biochem. / The nucleic acids of plant tissues. I. The extraction and estimation of deoxypentose nucleic acid and pentose nucleic acid by Ogur M. (1950)
Tauro P. E. Schweitzer R. Epstein and H. 0. Halvorson. 1969. Synthesis of macromolecules during the cell cycle in yeast p. 101-118. In G. M. Padilla G. L. Whitson and I. L. Cameron (ed.) The cell cycle. Academic Press Inc. New York. (10.1016/B978-1-4832-2797-9.50011-X)
Wallenfels K. 1962. B-Galactosidase (crystalline) p. 212-219. In S. P. Colowick and N. 0. Kaplan (ed.) Methods in enzymology vol. 5. Academic Press Inc. New York. (10.1016/S0076-6879(62)05207-6)
Warmsley , A. M. H. , and C. A. Pastemak . 1970 . The use of conventional and zonal centrifugation to study the life cycle of mammalian cells . Biochem. J. 19 : 493 - 499 . (10.1042/bj1190493) / Biochem. J. / The use of conventional and zonal centrifugation to study the life cycle of mammalian cells by Warmsley A. M. H. (1970)
Williamson , D. H. , and A. W. Scopes . 1960 . The behavior of nucleic acids in synchronously dividing cultures of Saccharomyces cerevisiae . Exp. Cell Res. 20 : 338 - 349 . (10.1016/0014-4827(60)90162-2) / Exp. Cell Res. / The behavior of nucleic acids in synchronously dividing cultures of Saccharomyces cerevisiae by Williamson D. H. (1960)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 8:38 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:01 p.m.)
Indexed	1 day, 18 hours ago (Sept. 4, 2025, 9:51 a.m.)
Issued	53 years, 9 months ago (Dec. 1, 1971)
Published	53 years, 9 months ago (Dec. 1, 1971)
Published Print	53 years, 9 months ago (Dec. 1, 1971)

Funders 0

None

BibTeX

@article{Sebastian_1971, title={Use of Yeast Populations Fractionated by Zonal Centrifugation to Study the Cell Cycle}, volume={108}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.108.3.1045-1050.1971}, DOI={10.1128/jb.108.3.1045-1050.1971}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Sebastian, J. and Carter, B. L. A. and Halvorson, H. O.}, year={1971}, month=dec, pages={1045–1050} }

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